@article { author = {Hassanloo, Thahereh and Jafarkhani Kermani, Maryam and Malmir Chegini, Mahsa and Sepehrifar, Roshanak and Mohajeri Naraghi, Sepehr and Miri, Seyyed Mehdy}, title = {Optimization of in vitro Propagation of Qare-Qat (Vaccinium arctostaphylus)}, journal = {Journal of Medicinal plants and By-product}, volume = {4}, number = {2}, pages = {225-231}, year = {2015}, publisher = {Iranian Medicinal Plants Society}, issn = {2322-1399}, eissn = {2588-3739}, doi = {10.22092/jmpb.2015.108913}, abstract = {In order to optimize the micropropagation of Qare-Qat (Vaccinium arctostaphylus J.J.Sm), samples were collected from two regions of Iran (Asalem and Kelardasht). Anderson (AN) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg L-1) and different levels of pH (4.5, 5 and 5.5) were compared. The in vitro shoots were transferred on AN media supplemented with zeatin (1 and 2 mg L-1) alone or in combination with Indole-3-butyric acid (IBA) (0.2 and 0.4 mg L-1). To optimize the rooting stage, elongated shoots (1.5- 2cm) were cultured on half strength semi- solid media containing activated charcoal (0 and 7g L-1), or half strength liquid AN medium supplemented with different concentrations of IBA (0, 0.5, 2 and 3 mg L-1) and NAA (0 and 0.5 mg L-1). The results indicated that at the establishment stage, AN media supplemented with 4 mg L-1 zeatin (pH= 5.5) and 1 mg L-1 zeatin (pH= 5) were the best treatment for Asalem and Kelardasht explants, respectively. For the Asalem explants, the highest number of proliferated shoots (4) was observed on AN media consisting of 2 mg L-1 zeatin and 0.4 mg L-1 IBA. Whereas for the Kelardash explants  the highest number of proliferated shoots (3) was observed on AN media consisting of 2 mg L-1 zeatin and 0.2 mg L-1 IBA. The highest percent of root formation (86 and 66%) were observed in half strength AN medium containing 7 g L-1 activated charcoal and 3 mg L-1 IBA and 0.5 mg L-1 NAA in Asalem and Kelardasht explants, respectively. The plantlets were successfully transferred to soil and the survival rate was 85%.}, keywords = {Medicinal plants,Micropropagation,in vitro,growth regulators,Vaccinium arctostaphylus}, url = {https://jmpb.areeo.ac.ir/article_108913.html}, eprint = {https://jmpb.areeo.ac.ir/article_108913_4a8413be0924b509a5e70c3a5cb118a3.pdf} }