Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Applications of Essential Oils in Food Preservation
115
122
EN
Iraj
Rasooli
Department of Biology, Shahed University, Opposite Imam Khomeini’s Shrine, Tehran-Qom Express Way, Tehran, Iran
rasooli@shahed.ac.ir
Zohreh
Rasooli
School of Medicine, Shahed University, Tehran, Iran
10.22092/jmpb.2013.108583
Many food products are perishable by nature and require protection from spoilage during their preparation, storage and distribution to give them desired shelf-life. Because food products are now often sold in areas of the world far distant from their production sites, the need for extended safe shelf-life for these products has also expanded. Currently, there is a strong debate about the safety aspects of chemical preservatives since they are considered responsible for many carcinogenic and teratogenic attributes as well as residual toxicity. For these reasons, consumers tend to be suspicious of chemical additives and thus the demand for natural and socially more acceptable preservatives has been intensified. One such possibility is the use of essential oils (EOs) as antibacterial additives. In the production of food it is crucial that proper measures are taken to ensure the safety and stability of the product during its whole shelf-life. In particular, modern consumer trends and food legislation have made the successful attainment of this objective much more of a challenge to the food industry. EOs comprise a large number of components and it is likely that their mode of action involves several targets in the bacterial cell. It is most likely that their antibacterial activity is not attributable to one specific mechanism but that there are several targets in the cell. The potency of naturally occurring antimicrobial agents or extracts from plants, ranges of microbial susceptibility and factors influencing antimicrobial action and their antioxidative properties, aimed at food preservation, are reviewed in this article. Methods employed for estimation of inhibitory activity, mode of action and synergistic and antagonistic effects are evaluated. Hence, it is recommended that more safety studies be carried out before EOs are more widely used or at greater concentrations in foods that at present. There is therefore scope for new methods of making food safe which have a natural or ‘green’ image.
Antimicrobials,Essential oils,food,Safety,Toxicity
https://jmpb.areeo.ac.ir/article_108583.html
https://jmpb.areeo.ac.ir/article_108583_8d614005aca611c55f8af2359f76156a.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
The Essential Oil Composition and Antioxidant Activity of Achillea spp. Growing in the Southwest of Iran
123
130
EN
Roozbeh
Farhoudi
Department of Agronomy and Plant Breeding, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran
rfarhoudi@gmail.com
Mohammad Amin
Mehrnia
Department of Food Industry, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran
maminm@gmail.com
10.22092/jmpb.2013.108584
The composition of essential oil isolated from <em>Achillea eriophora, Achillea</em><em> millefolium, </em><em>Achillea</em><em> biebersteinii </em>and<em> Achillea tenuifolia</em> growing wild in the south west of Iran, was analyzed. <em>A. eriophora, A.</em><em> millefolium</em> and <em>A. tenuifolia</em> essential oils were characterized by sabinene, 1, 8-cineole, terpinene-4-ol, ά –bisabolol, p-Cymene, β-pinene and α-pinene. The<em> A.</em><em> biebersteinii</em> essential oil was characterized by sabinene, borneol, camphor, piperitone and α-pinene. Antioxidant activity was analyzed using the 1,1-diphenyl-2- picrylhydrazyl free radical scavenging and Fe <sup>3+</sup> reducing power methods. Results indicated essential oil obtained from <em>A. eriophora, A. </em><em>millefolium, A. tenuifolia</em> and<em> A.</em><em> biebersteinii</em> exhibited a dose-dependent increase with a radical scavenging effect of 85.0%, 82.0% , 82.0% and 64.0% at 350 μg/ml, which are close to the 1,1-diphenyl-2- picrylhydrazyl inhibition of the positive control Butylated Hydroxytoluene (88.0%) at the same concentration. It was shown that the <em>A.</em><em> biebersteinii</em> essential oil exhibited the weakest antioxidant effect than Butylated Hydroxytoluene or other <em>Achillea </em>spp. essential oils. In this study chamazulene, ά-bisabolol and ά –bisabolol oxide percentage were higher in <em>A. eriophora, A. </em><em>millefolium</em> and <em>A. tenuifolia</em> essential oil compared to <em>A.</em><em> biebersteinii</em> essential oil and these compounds improved antioxidant capacity of <em>Achillea</em> spp<em>.</em>
Achillea spp,essential oil,Radical scavenging,Fe 3+ reducing power
https://jmpb.areeo.ac.ir/article_108584.html
https://jmpb.areeo.ac.ir/article_108584_3dd958066142bf85f97a16ba634ff64a.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Secondary Metabolite Contents and Antioxidant Enzyme Activities of Cichorium intybus Hairy Roots in Response to Zinc
131
138
EN
Bentolhoda
Azarmehr
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
Farah
Karimi
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
fkarimi@shahed.ac.ir
Masoud
Taghizadeh
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
Seyed Latif Mousavi
Gargari
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
10.22092/jmpb.2013.108585
Hairy root systems are formed by transforming plant tissues with the “natural genetic engineer” <em>Agrobacterium rhizogenes. </em>In most plants such as <em>Cichorium intybus </em>L<em>.</em>, hairy root cultures have proven to be an efficient system for secondary metabolites production. The effect of Zinc (ZnSO<sub>4</sub>), a heavy metal, was investigated at different concentrations (0, 1, 5 and 10 mM) on some secondary metabolite contents at three time course levels (24, 48 and 72 h).The treated hairy roots of chicory were compared with control and with each other in growth rate, phenol flavonoid and chicoric acid production rate. In addition, antioxidant enzyme activities were determined. Results showed decreased hairy roots weights and increased phenol, flavonoid, chicoric acid and antioxidant enzyme activities in response to higher concentrations of Zinc at higher time courses. Also, an increase in chicoric acid release into the culture media was observed that is important for industrial uses.
hairy root,Cichorium intybus,Secondary metabolites,Antioxidant enzymes,ZnSO4
https://jmpb.areeo.ac.ir/article_108585.html
https://jmpb.areeo.ac.ir/article_108585_6aa2a7c4158ba841b02c77e9526084b0.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Study of the Chemical Composition of Essential Oils of Perovskia abrotanoides Karel at the Different Stage and Distillation by Gas Chromatography
139
142
EN
Sedigheh
Kolbady Nejad
M.Sc. Student of Payame Noor University, Tehran,Iran.
Kamkar
Jaimand
0000-0001-6519-7420
Phytochemistry Group, Department of Medicinal Plants & By-products, Research Institute of Forests and Rangelands, P.O. Box 13185, Tehran, Iran
jaimand@rifr-ac.ir
Azam
Monfared
Academic member of Payame Noor University, Tehran, Iran.
Mohammad
Akbarzadeh
Mazanderan Research Center for Agriculture and Natural Resources, Mazanderan, Iran.
10.22092/jmpb.2013.108586
Medicinal plante Borazambol with the scientific name of <em>Perovskia abrotanoides </em>Karel. belongs to the family Lamiaceae. It is growing wild in the margin of mountainous roads of arid and cold climate of north Iran. It is for a long time that indigenous people by different methods in traditional medicine use its products in preventing and curing diseases. In this research, essential oils of <em>Perovskia abrotanoides </em>Karel, extracted and measured at the time of flowering stage and vegetative stage by different methods of distillationand then were analyzed by GC and GC/MS. The essential oil yield at vegetative stage with hydro-distillation was 2.2%, water & steam distillation (Kyzer & Long) 5.7% and steam distillation 2.5%, respectively. Major component identificated by water distillation (Clavanger) were α- terpineol (32%), n-octanol (22.5%), myrcene (7.2%), and by water and water & steam distillation (Kyzer & Long) were α- terpineol (30.3%), n-octanol (20.1%), myrcene (7.2%), and by steam distillation were α- terpineol (26.2%), n-octanol (17.4%), n-pentadecane (8.2%). The essential oil yield at flowering stage with hydro-distillation was 1.9%, water & steam distillation (Kyzer & Long) 1.5% and steam distillation 1%, respectively. Major component identificated by water distillation (Clavanger) were n-octanol (23.3%); α- terpineol (21.9%) and (Z)-β- ocimene (15.5%), and by water and steam distillation (Kyzer & Long) were α- terpineol (19.9%), n-octanol (19.9%), (Z)-β- ocimene (13.6%), and by steam distillation were n-octanol (16.6%), α- terpineol (15.3%), and (Z)-β- ocimene (13.5%).
essential oil,Perovskia abrotanoides Karel,flowering stage,vegetative stage,GC,GC/MS
https://jmpb.areeo.ac.ir/article_108586.html
https://jmpb.areeo.ac.ir/article_108586_e3d4ad10558f865d213b29a7d4624269.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Effect of Nutrition and Harvest Time on Growth and Essential Oil Content of Thymus vulgaris L.
143
151
EN
Bohloul
Abbaszadeh
0000-0002-1292-7415
Research Institute of Forests and Rangelands, Tehran, Iran
babaszadeh@rifr-ac.ir
Masoumeh
Layegh Haghighi
Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran
10.22092/jmpb.2013.108587
Thyme (<em>Thymus vulgaris</em> L.) is an important medicinal plant with highly valuable essential oils. In this study, effects of different fertilizer treatments and cuts on <em>Thymus vulgaris</em> L. was evaluated in 2008-2009. The research was conducted at Alborz Research Station, Research Institute of Forests and Rangelands, Karaj, Iran. The Experimental design was split plot in time in the form of a randomized complete block design with three replications. The main factor was nutritionin 16 levels consisting of different combinations of nitrogen (N), phosphorus (P), potassium (K) and manure (M): N<sub>0</sub>P<sub>0</sub>K<sub>0</sub>M<sub>0</sub>, N<sub>40</sub>P<sub>32</sub>K<sub>40</sub>M<sub>0</sub>, N<sub>80</sub>P<sub>64</sub>K<sub>80</sub>M<sub>0</sub>, N<sub>120</sub>P<sub>96</sub>K<sub>120</sub>M<sub>0</sub>, N<sub>160</sub>P<sub>128</sub>K<sub>160</sub>M<sub>0</sub>, N<sub>140</sub>P<sub>112</sub>K<sub>140</sub>M<sub>5</sub>, N<sub>120</sub>P<sub>96</sub>K<sub>120</sub>M<sub>10</sub>, N<sub>100</sub>P<sub>80</sub>K<sub>100</sub>M<sub>15</sub>, N<sub>80</sub>P<sub>64</sub>K<sub>80</sub>M<sub>20</sub>, N<sub>60</sub>P<sub>48</sub>K<sub>60</sub>M<sub>25</sub>, N<sub>40</sub>P<sub>32</sub>K<sub>40</sub>M<sub>30</sub>, N<sub>20</sub>P<sub>16</sub>K<sub>20</sub>M<sub>35</sub>, N<sub>0</sub>P<sub>0</sub>K<sub>0</sub>M<sub>40</sub>, N<sub>0</sub>P<sub>0</sub>K<sub>0</sub>M<sub>30</sub>, N<sub>0</sub>P<sub>0</sub>K<sub>0</sub>M<sub>20</sub> and N<sub>0</sub>P<sub>0</sub>K<sub>0</sub>M<sub>10</sub> (indices following the letters N, P and K are application rates in kg/ha, indices following the letter M are the application rates of manure in t/ha). The sub factor was harvest (cut) time (late May and early September). Results indicated that fertilizer, harvest date and their interactions significantly affected most of the traits. Mean comparison of the interactions indicated that treatments containing both chemical fertilizers and manure showed positive effects, although the most plant height was achieved with N<sub>160</sub>P<sub>128</sub>K<sub>160</sub>M<sub>0</sub> × 1<sup>st</sup> cut, in both years (32.5 cm in 2008 and 32.66 cm in 2009). Essential oil yield was the highest in 2008 in N<sub>120</sub>P<sub>96</sub>K<sub>120</sub>M<sub>10</sub> × 2<sup>nd</sup> cut (26.01 kg/ha), and in 2009 in N<sub>100</sub>P<sub>80</sub>K<sub>100</sub>M<sub>15</sub> × 1<sup>st</sup> cut (26.79 kg/ha). Generally, it can be concluded from the results that <em>T. vulgaris</em> responds well to fertilization, and selecting the best treatment depends on the objective of production.
essential oil,P-cymene,Thyme,Thymol,γ-terpinene
https://jmpb.areeo.ac.ir/article_108587.html
https://jmpb.areeo.ac.ir/article_108587_c1879532558deff977bf757aa1383488.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Evaluation of Essential Oil Yield, Morphological and Phenological Traits in Some Populations of Two Chamomile Species (Matricaria recutita and M. aurea)
153
158
EN
Negin
Adeli
Islamic Azad University, Karaj Branch, Karaj, Iran
Mohammad Ali
Alizadeh
Research Institutes of Forests and Rangelands, Tehran, Iran
alizadeh202003@gmail.com
Ali Ashraf
Jafari
Research Institutes of Forests and Rangelands, Tehran, Iran
10.22092/jmpb.2013.108588
Evaluation of essential oil yield, morphological and phenological traits of seven populations of two<strong> </strong>Chamomile<strong> </strong>species (<em>Matricaria aurea </em>and<em> M. recutita</em>)<em>,</em> were carried out using Randomized Completely Blocks Design (RCBD) in three replications in Alborz research station in Karaj, Iran during 2011-2012. Data were collected for 12 morphological and phenological traits. Analysis of variance showed significance difference (P ≤%1) between populations for all of traits. Also, the effect of year and year x population interaction effect were significant (P≤%1) for all of traits. Results showed <em>Matricaria</em><em> recutita</em> hah higher values for all of traits except shoot fresh and dry weight. Mean comparison showed that two populations of Esphahan and Hamadan from <em>Matricaria recutita</em> and one population Gachsaran of<em> Matricaria</em> <em>aurea</em> had high shoot yield and essential oil yield than other populations. Result of simple correlation analysis showed that essential oil percentage was positively correlated with plant height, flower number, shoot dry weight and essential oil yield and negatively correlated with time of flowering and maturity date and GDD (Growth Degree Days), suggesting that early maturity plants had higher essential oil content. Results of stepwise regression analysis for essential oil content as dependant variables and other traits as independent variables showed significant effects of maturity date, flower number, plant height, canopy diameter length and flowering date in essential oil content.
Chamomile,Matricaria recutita,M. aurea,essential oil
https://jmpb.areeo.ac.ir/article_108588.html
https://jmpb.areeo.ac.ir/article_108588_3a9a244adb05d5bf77f8ac01a10dc491.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Evaluation of Basil (Ocimum basilicum L.) Essential Oil Content and Yield under Different Plant Densities and Nitrogen Levels
159
162
EN
Amir Mohammad
Daneshian Moghaddam
Department of Field Crops, Faculty of Agriculture, Shabestar Branch-Islamic Azad University, Shabestar, Iran
daneshian.a.m@gmail.com
Bilal
Gürbüz
Department of Field Crops, Faculty of Agriculture, Ankara University, Ankara, Turkey
10.22092/jmpb.2013.108589
A study was conducted to investigate the effect of different planting densities and nitrogen doses on essential oil content and yield in herb and leaves of basil (<em>Ocimum basilicum</em> L.). The research was conducted during 2007 and 2008 using split-plot randomized complete block design with four replications. The experiment consisted of four plant densities (30×20, 40×20 and 50×20 cm) as the main plot and four nitrogen levels (0, 50, 100 and 150 kg/ha) as the sub plots. Results showed plant density did not have significant effect on essential oil content and essential oil yield in herb and leaves. Mean essential oil ratio in herb was 0.49, 0.44%; essential oil ratio in leaf 0.59, 0.54%; essential oil yield in herb 19.4, 23.7 l/ha and essential oil yield in leaf was 12.8, 16.1 l/ha during 2007 and 2008, respectively. The highest essential oil ratio in herb was obtained from 40×20 cm plant density and 50 kg ha<sup>-1</sup> N during 2007. Nitrogen fertilizer had statistically insignificant effect on oil ratio of herb and leaves during two years of experiment. The highest essential oil yield of herb was obtained from low density(30×20 cm) using 100 kg ha<sup>-1</sup> N fertilizer. In General, essential oil yield during 2008 with three cuts was better compared to 2007 with two cuts.
Basil (Ocimum basilicum),Essential oil ratio,essential oil yield,Nitrogen doses,plant density
https://jmpb.areeo.ac.ir/article_108589.html
https://jmpb.areeo.ac.ir/article_108589_0e2730902d6e1d7643c8b27be539c1c1.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Treatment with Essential Oils Extends the Vase Life of Cut Flowers of Lisianthus (Eustoma grandiflorum)
163
169
EN
Hassan
Bayat
Department of Horticultural Science, Faculty of Agriculture, Ferdowsi University of Mashhad, PO Box 91775-1163 Mashhad, Iran
Reza
Geimadil
Department of Horticultural Science, Faculty of Agriculture, Ferdowsi University of Mashhad, PO Box 91775-1163 Mashhad, Iran
r.geimadil@gmail.com
Ali
Abdollahi Saadabad
Department of Horticultural Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
10.22092/jmpb.2013.108590
Lisianthus (<em>Eustoma grandiflorum</em> cv. ‘Mriachii Blue’) is one of the most important and marketable cut flowers in the world. However, a relatively limited vase life reduces its marketability. The aim of the present study was to evaluate the effects of essential oils in extending vase life of cut flowers of Lisianthus. For this purpose a factorial experiment based on a completely randomized design with <em>Zataria multiflora </em>and <em>Echinophora platyloba</em> essential oils at (0, 100, 200 ppm) with 3 replications, was conducted. The results showed that addition of essential oils to vase solutions increased vase life, petal water content, leaf relative water content and SPAD value, significantly. The highest vase life (14.5 days) was observed in the vase solution containing 200 ppm <em>E. platyloba</em> plus 100 ppm <em>Z. multiflora</em> essential oils with 95% increase as compared to the control. All essential oil treatments resulted in higher relative fresh weight as compared to the control; however the highest relative fresh weight was observed in cut flowers treated with <em>E. platyloba</em> essential oil at 200 ppm with 54% increase over the control. It can be safely concluded that essential oils as natural, safe and biodegradable compounds are suitable alternatives to conventional chemical treatments in order to prolong vase life of cut flowers of Lisianthus.
Essential oils,Medicinal plants,postharvest,Relative fresh weight,Vase life,Lisianthus
https://jmpb.areeo.ac.ir/article_108590.html
https://jmpb.areeo.ac.ir/article_108590_827152f47bfb9595f5af8e650c5aaf41.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Allelopathic Effect of Artemisia herba-alba Asso. Essential Oil on Seed Germination of Agropyron desertorum and Agropyron cristatum
171
176
EN
Ghasem Ali
Dianati Tilaki
Department of Rangeland Management, Faculty of Natural Resources, Tarbiat Modares University, Tehran, Iran
dianatig@modares.ac.ir
Reza
Bahari Balkhkanloo
Department of Rangeland Management, Faculty of Natural Resources, Tarbiat Modares University, Tehran, Iran
Mohammad-Bagher
Rezaee
Phytochemistry Group, Department of Medicinal plants & By-products, Research Institute of Forest and Rangelands, P.O.Box 1318, Tehran, Iran
mb.rezaee@gmail.com
Masoumeh
Amirkhani
Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
10.22092/jmpb.2013.108591
This study was conducted to investigate allelopathic effect of the essential oil of <em>Artemisia herba-alba </em>Asso., on seed germination characteristics of<em> Agropyron desertorum </em>and<em> Agropyron cristatum</em> in a laboratory experiment. Essential oil was extracted from the aerial parts of <em>Artemisia herba-alba </em>using a Clevenger-Type apparatus. The volatile chemical compositions of <em>Artemesia herba-alba</em> were determined with gas chromatography–mass spectrometry (GC–MS). The crude essential oil was diluted with ethanol to a final concentration of 100 ppm, 500 ppm, 1000 ppm, 1500 ppm and 2000 ppm. Seed germination test was carried out on filter paper moistened with 5 ml of different dilutions of essential oil or distilled water as control. The results showed that increase in essential oil concentration reduced root lengths, seed germination percentage and vigor index of <em>Agropyron desertorum</em>and<em> Agropyron cristatum<strong>.</strong> </em>
Allelopathy,essential oil,Agropyron desertorum,Seed germination,chemical composition,Vigor index
https://jmpb.areeo.ac.ir/article_108591.html
https://jmpb.areeo.ac.ir/article_108591_047509d9050de73cf81f7d036f8d8ec7.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Improvement of Hypericin and Hyperforin Production Using Zinc and Iron Nano-oxides as Elicitors in Cell Suspension Culture of St John's wort (Hypericum perforatum L.)
177
184
EN
Ebrahim
Sharafi
Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran
Seyyed Mojtaba
Khayam Nekoei
Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran
Mohamad Hossein
Fotokian
Department of Agricultural Biotechnology, Shahed University, Tehran, Iran
Dariush
Davoodi
Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran
Hossein Hadavand
Mirzaei
Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran
Tahereh
Hasanloo
Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P. O. Box 31535-1897, Karaj, Iran
thasanloo@abrii.ac.ir
10.22092/jmpb.2013.108592
Zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in<em> Hypericum perforatum</em> cell suspension culture. High performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in <em>H. perfuratum</em> cell suspension cultures elicited with different concentrations of zinc and iron nano-oxide (0, 50, 100 and 150 ppb) after 72 h. In the cultures stimulated by zinc nano-oxide, the hypericin and hyperforin production reached to the maximum (7.87 and 217.45 µg/g DW, respectively), which were 3 and 13-fold higher than the control. The amount of hypericin and hyperforin was increased from 2.07 and 16.27 µg/g DW to 11.18 and 195.62 µg/g DW in iron nano-oxide treated cultures. The cell cultures treated with zinc and iron nano-oxides showed increased hyperforin production as compared to the hypericin production. These observations suggested that nano-particles can be appropriate candidates for elicitation studies of <em>in vitro</em> secondary metabolites production.
cell suspension,Hypericum perfuratum,Hypericin,hyperforin,Nano-particles
https://jmpb.areeo.ac.ir/article_108592.html
https://jmpb.areeo.ac.ir/article_108592_620a6985072128289a9cceb030b6e07c.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Assessment of Genetic Diversity among Wild Populations of Achillea bieberstenii Afan. Using Agro-morphological and Germination Traits
185
195
EN
Masoomeh
Izadpanah
Research Institute of Forests and Rangelands Gene Bank, Tehran, Iran
izad_2006@yahoo.com
Parvin
Salehi Shanjani
Research Institute of Forests and Rangelands Gene Bank, Tehran, Iran
psalehi@rifr-ac.ir
Ali
Ashraf Jafari
Research Institute of Forests and Rangelands Gene Bank, Tehran, Iran
10.22092/jmpb.2013.108593
There is substantial diversity in<em> Achillea biebersteinii</em> plants in Iran. It has considerable different biological activities including antibacterial<em>, </em>antifungal<em>, </em>antioxidant<em>, </em>insecticidal<em>, </em>herbicidal and wound healing. Research improvement for medicinal plants depends on the available genetic diversity. The assessment of genetic variability could increase the information for breeding programs. To evaluate possible diversity among different populations of <em>A.</em> <em>biebersteinii</em><em>, </em>agro-morphological and germination traits of the populations were investigated. To evaluate agro-morphological traits<em>, </em>seeds of 22 populations were sown in the field. The experiment was laid out using Randomized Complete Block Design (RCBD) with three replications. A total of six agro-morphological traits including plant height<em>, </em>plant diameter<em>, </em>lateral shoot number<em>, </em>flower diameter<em>, </em>flower number and shoot yield<em>, </em>were collected. For evaluation of seed germination traits<em>, </em>seeds of 10 populations were sown in standard condition ((20±2 °C) and pre-cold treatment (7 days at 4 °C followed by standard condition). The seed germination traits were studied as hypocotyl length<em>, </em>radicle length<em>, </em>radicle to hypocotyl length ratio<em>, </em>seedling length<em>, </em>germination percentage,germination rate and vigor index. Based on the results the population 20187 (Golestan) had the highest value for all of agro-morphological traits. For germination traits the populations 10140 (Minoodasht) and 17310 (Shahrood) were the best performing populations. The result showed that pre cold treatment reduced radicle length in most cases but population 17310 (Shahrood) produced significantly longer radicle under pre-cold treatment. This population was recommended for dry cold regions.
Achillea biebersteinii,Agro-morphology,germination,cold Treatment
https://jmpb.areeo.ac.ir/article_108593.html
https://jmpb.areeo.ac.ir/article_108593_9769eba69c616bd25f1bfc0e0016218d.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Effects of Arginine Pretreatments on Oxidative Stress Damages and Alkaloid Content in Roots of Hyoscyamus niger under Nickel Stress
197
204
EN
Fatemeh
Nasibi
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
nasibi2002@yahoo.com
Zahra
Asrar
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
Tayebeh
Heidari
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
10.22092/jmpb.2013.108594
Heavy metal pollution is a worldwide problem with serious environmental consequences. The objective of the present experiment was to investigate whether arginine as nitric oxide precursor and or polyamines substrate can decrease the destructive effects of oxidative stress induced by nickel contamination in <em>Hyoscyamus niger </em>plant. In this study the effects of arginine pretreatment on alkaloid content of <em>Hyoscyamus</em> plant under heavy metal stress were investigated. In this research, four weeks seedlings were pretreated with 10 or 20 µmol arginine and then subjected to 50 or 100 µmol Ni solutions. Results showed that hydrogen peroxide content, lipoxigenase, catalase and guaiacol peroxidase activity increased in those plants which were under Ni stress, while ascorbate peroxidase activity did not change. Alkaloids content decreased in Ni stressed plants. Arginine pretreatment decreased the amounts of hydrogen peroxide and activity of these enzymes in stressed plants when compared with non-pretreated plants. Proline content also increased in Ni-stressed plants while arginine pretreatment decreased the proline content. Pretreatment of plants with arginine increased the amounts of total alkaloids in plants which were under Ni stress. In this study, it seems that protective effects of arginine were related to either polyamines or indirect synthesis of NO from polyamines.
Antioxidant enzyme,Heavy metal,nitric oxide,Polyamines,Proline
https://jmpb.areeo.ac.ir/article_108594.html
https://jmpb.areeo.ac.ir/article_108594_23bec772d4a64f6b4499d8a42e8b138f.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Essential Oil Content and Composition of Lemon Verbena (Lippia citriodora Kunth.) during Different Phenological Stages
205
208
EN
Reza
Shahhoseini
Department of Horticulture, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
Arman
Beyraghdar
Department of Horticulture, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
Seyed-Razi
Karimi
Department of Agricultural Mechanics, Tarbiat Modares University, Tehran, Iran
Mohammad-Taghi
Ebadi
Department of Horticulture, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
m.t.ebadi@gmail.com
10.22092/jmpb.2013.108595
In this study, aerial parts of <em>Lippia citriodora</em> Kunth were harvested at three phenological stages (i.e. vegetative, full flowering and fruit set). The essential oil isolated by hydro distillation for 3 h using a Clevenger-type apparatus and the oil was analyzed by GC and GC-MS. The yields of oils (w/w%) in different stages was in the order of: full flowering (0.9%), vegetative (0.48%) and fruit set (0. 25%). In total, 13, 12 and 11 compounds of essential oil were identified in vegetative, full flowering and fruit set, respectively. The main compounds in three stages were geranial and neral. Geranial was highest at vegetative (33.7%) and lowest at full flowering (32.7%) stages. The highest and lowest neral content was observed at vegetative (26.1%) and fruit set (25.06%) stages, respectively.
Lemon verbena,Lippia citriodora Kunth,phenological stages,geranial,Neral,Verbenaceae
https://jmpb.areeo.ac.ir/article_108595.html
https://jmpb.areeo.ac.ir/article_108595_117e4c4a5a34f756e5c3fdb5fe7bfa6e.pdf
Iranian Medicinal Plants Society
Journal of Medicinal plants and By-product
2322-1399
2588-3739
2
2
2013
09
01
Induction of Callus and Somatic Embryogenesis from Cotyledon Explants of Fagonia indica Burm.
209
214
EN
Mohammad Ali
Ebrahimi
Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran.
ma_ebrahimi@pnu.ac.ir
Aida
Payan
Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran.
10.22092/jmpb.2013.108596
To explore the potential for <em>in vitro </em>rapid regeneration of <em>Fagonia indica </em>Burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on MS medium which supplemented with 1.0-5.0 µM 2,4-D. Callus was initiated from cotyledon explant on MS medium supplemented in all concentrations of 2,4-D investigated. 2,4-D was more effective than IAA and NAA for callus induction, but auxins and cytokines’ combination is essential for the green callus induction for somatic embryogenesis. Induced calluses were subcultured on media containing 2.5-5.0 µM BA or Kin combined with different concentrations IAA, NAA and 2,4-D. Elimination of 2,4-D and a slight increase of BA concentration induced of the relating somatic embryogenesis. The maximum globular structure embryos were further enlarged and produced the somatic embryos in MS basal medium supplemented with 5.0 BA µM and also combined with either 2.5 µM NAA. Continued formation of the globular embryo occurred in these mediums for 12 months. The maximum germinations of embryos were observed in MS medium + 1.5% sucrose without growth regulators.
Fagonia indica Burm. f,Somatic Embryogenesis,Callus
https://jmpb.areeo.ac.ir/article_108596.html
https://jmpb.areeo.ac.ir/article_108596_ac0b0e482635c490c38dc0dcbc2bb431.pdf