Antimicrobial Screening of Fennel at the Seedling Stage

Authors

1 Department of Biology, Collage of Sciences, Shiraz University, Shiraz 71454, Iran

2 Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-Cho, Kagawa 761-0795, Japan

Abstract

A wide range of medicinal plant parts that possess varied medicinal properties are used for extracts as raw drugs. In this study, the ethanolic extract obtained from aerial parts of fennel (Foeniculum vulgare Mill.) seedlings was evaluated in vitro to examine their antimicrobial activity against four Gram-negative and three Gram-positive bacteria and two fungi. Aerial parts of one-month-old seedlings were air dried and powdered. Each powdered sample (20 g) was extracted with 200 ml ethanol (96%) using a shaking water bath for 24 h at room temperature. The solvent was removed under vacuum at 40°C using a rotary vacuum evaporator. The antimicrobial effect of the aerial part of fennel seedlings was tested by the disc diffusion method. The experiment was a randomized complete block with three replicates for each sample. Fennel seedling extract had no inhibitory effect against Gram-positive bacteria and fungi, but had a weak effect against Gram-negative bacteria.

Keywords

References

1. Cowan MM. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 1999;12:564-582.
2. Kaur GJ, Arora DS. Bioactive potential of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi belonging to the family Umbelliferae. J. Med. Plants Res. 2010;4:87-94.
3. Farooqi AA, Seeramu BS. Cultivation of medicinal and aromatic crops. New Delhi-India: University Press; 2001.
4. Garg C, Khan SA, Ansari SH, Garg M. Efficacy and safety studies of Foeniculum vulgare through evaluation of toxicological and stadardisation parameters. Int. J. Pharm. Sci. 2010;2:43-45.
5. Uniyal SK, Singh KN, Jamwal P, Lal B. Traditional use of medicinal plants among the tribal communities of Chhota Bhanga, Western Himalayan. J. Ethnobiol. Ethnomed. 2006;2:1-14.
6. Anwar F, Ali M, Hussain AI, Shahid M. Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare Mill.) seeds from Pakistan. Flavour Fragrance J. 2009; 24:170-176.
7. Boytop T. Therapy with medicinal plants in Turkey (past and present). 1st ed. Istanbulm-Turkey: Publication of Istanbul University; 1999.
8. Guilled MD, Manzanons MJ. A study of several parts the plant Foeniculum vulgare as a source of compounds with industrial interests. Food Res. Int. 1996;29:85-88.
9. Muck B, Foechterlen D, Reduron JP, Danton P, Hildenbrand M. Phytochemical and chemotaxonomic studies of Foeniculum vulgare. Biochem. Syst. Ecol. 1997;25:353-358.
10. Epstein E. Mineral nutrient of plants: principles and perspectives. New York: Wiley Publication; 1972.
11. Bauer AW, Kirby WMM, Sherries JC, Turck M. Antibiotic susceptibility testing by single disk method. Am J. Clin. Pathol. 1966;45:493-496.
12. Janssen AM, Scheffer JJC, Svendsen AB. Antimicrobial activity of essential oils a literature review aspects of the test methods. Planta Med. 1987;53:395-398.
13. Al-Ismail K, Hamdan M, Al-Delaimy K. Antioxidant and anti-Bacillus cereus activities of selected plant extracts. Jordan J. Agr. Sci. 2006;2(2):64-74.
14. Kwon YS, Choi WG, Kim WJ, Kim WK, Kim MJ, Kang WH, Kim CM. Antimicrobial constituents of Foeniculum vulgare. Arch. Pharm. Res. 2002;25:154-157.
Journal of Medicinal plants and By-product
Volume 1, Issue 1
April 2012
Pages 75-77

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