Effect of Different Treatments on Breaking Dormancy of Teucrium chamaedrys L. Seed

Authors

1 Department of Crop Production and Plant Breeding, College of Agricultural Science, Shahed University, Tehran, Iran

2 Agriculture Biotechnology, College of Energy Engineering and New Technologies, University of Shahid Beheshti, Tehran, Iran

Abstract

In order to find the most effective method to overcome seed dormancy in Teucrium chamaedrys  L. medicinal plant species the effect of some chemical and physical breaking methods including: cold stratification (5 °C), scarification with sandpaper, stratification with needle, concentrated sulfuric acid, gibberellic acid and combination of these methods were investigated. Treated seeds along with control were sown in the germinator at 25±2 °C for 45 days according to a randomized complete block design with three replications. Results showed that all seed treatments increased seed dormancy, however, the highest germination percentage and lowest mean germination time obtained when seeds were treated with sulfuric acid followed by chilling and gibberellic acid, respectively. Sulfuric acid increased the permeability of the seed coat and the effect of gibberellic acid was enhanced by cold treatment. Results showed that T. chamaedrys seeds have both physical and physiological dormancy.

Keywords


1. Leadem, CL. Dormancy-Unlocking seed secrets. U S Forest Service General Technical Report Pnw. 1997;0:43-52.
2. Nasiri M, Babakhanloo P, Maddah-Arefi H. First report on braking dormancy and seed germination on Diplotaenia damavandica Mozaffarian. Iran. Rangeland. Forest. Plant Breed. Genetic Res. 2003;2:258-274. (In Persian)
3. Emery DE. Seed propagation of native California plants. 1987. Santa Barbara Botanic Garden, Santa Barbara, CA.
4. Ljubuncic P, Dakwar S, Portnaya I, Cogan U, Azaizeh H, Bomzon A.. Aqueous Extracts of Teucrium polium possess remarkable antioxidant activity in vitro. Evidence-based Complementary and Alternative Medicine. 2006;3:329-338.
5. Said O, Khalil K, Fulder S, Azaizeh H. Ethnopharmacological survey of medicinal herbs in Israel, the Golan Heights and the West Bank region. Ethnopharmacol. 2002;83:251-265.
6. Suleiman MS, Abdul-Ghani AS, Al-Khalil S, Amin R. Effect of Teucrium polium boiled leaf extract on intestinal motility and blood pressure. Ethnopharmacol. 1988;22:111-116.
7. Autore G, Capasso F, De Fusco R, Fasulo M P, Lembo M, Mascolo N, Menghini, A. Anti pyretic and antibacterial actions of Teucrium polium L. Pharmacol. Res Commun. 1984;16:21-29.
8. Panovska,T.K., Kulevanova, S., Stefova, M., . In vitro antioxidant activity of crystalization of berberine in the liquid medium of Thalictrum minus cell suspension cultures. Plant Cell Rep. 2005;3:254-257.
9. Capasso F, Cerri R, Morrica P, Senatore F. Chemical composition and anti-inflammatory activity of an alcoholic extract of Teucrium polium L. Bollettino Della Societa Italian di biologia sperimentale. 1983;59:1639-1643.
10. Shakeri-Almoshiri M, Mianabadi M, Yazdanparast R. Effect of different treatments on seed dormancy of Teucrium polium. Iran Rangeland Forest Plant Breed Genetic Res. 2009;17:100-111. (In Persian)
11. Razavi MS, Hajiboland R. Dormancy breaking and germination of Prangos ferulaceae seeds. Eur Asia J Bio Sci. 2009;3:78-83.
12. Najdafi F, Bannayan M, Tabrizi L, Rastgoo M. Seed germination and dormancy breaking techniques for Ferula gummos and Teucrium polium. Arid Environ. 2006;64:542-547.
13. Benvenuti S, Ceccarini L, Macchia M. Germination ecology of Teucrium marum L: an endemic species of the tuscany archipelago. ISHS Acta Horticulture 723: I International Symposium on the Labiatae: Advances in Production, Biotechnology and Utilisation. 2006.

14. International Seed Testing Association (ISTA). International Rules for Seed Testing. Seed. Sci.Tech. 1999;  27: Supplement, pp:333

15. Villiers TA. Dormancy and the survival of plants. Edward Arnold publishers limited. London. 1978.

16. Taylor J S, Wareing PF. The effect of puncturing on the endogenous levels of gibberellins and cytokinins in seeds of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and sugar pine (Pinus lambertiana Dougl.). Plant Cell Environ. 1979;2:165-171.

17. Dvazdah Emami S, Shah Mansouri A. The effect of cold on seed germination of some medicinal herb. Proceedings of the Second Conference on Medicinal Plants. Shahed University. 2003;8-7 Feb, p297. (In Persian).

18. Hidayati SN, Baskin J M, Baskin C. C. Morphophysiological dormancy in seeds of two north American and one Eurasian species of Sambucus (Caprifoliaceae) with underdeveloped spatulate embyos. American. Bot. 2000;87:1669-1678.

19. Widrlechner MP, Kovach DA. Dormancy- breaking protocols for Cuphea seed. Seed Sci Tech. 2000;28:11-27.

20. Iglesias RG, Babiano MJ. Endogenous abscisic acid during the germination of chickpea seed. Physiologia Plant. 1997;100:500-504.