Antibacterial Properties of Ajuga chamaecistus Subsp. Scoparia and Chemical Composition of its Oils

Authors

1 Essential Oils Research Institute, University of Kashan, Kashan, Iran

2 Young Researchers and Elite Club, Kashan Branch, Islamic Azad University, Kashan, Iran

3 Isfahan Research Center of Natural Sources, Kashan Station, Kashan, Islamic Republic of Iran

Abstract

In the present study, we reported the essential oils chemical composition and antibacterial activities of the aerial parts of Ajuga chamaecistus Ging. ex Benth. Subsp. Scoparia (Boiss.) Rech.f. thatwere collected duringMay 2013 and April 2014 and extracted by SDE (simultaneous distillation–extraction) and Clevenger apparatus. GC/MS analysis of the plant essential oils led to the identification of chemical composition of its oils. The main constituents of the essential oils in two SDE (simultaneous distillation–extraction) and Clevenger apparatus were β-Pinene (23.5%), α-Pinene (6.9%), Limonene (10.8%), Linalool (8.3) and Eugenol (7.7%). Essential oil was tested for their antibacterial activities using Gram-negative bacteria, Gram-positive bacteria. The plant was screened for its antibacterial activity and showed antibacterial activity against P. aeruginosa, E. coli, K. pneumoniae, P. vulgaris, S. paratyphi, B. subtilis, S. aureus, S. epidermidis and S. dysenteriae.

Keywords


1. Camps F, Coll J. Insect allelochemicals from ajuga plants, Phytochem. 1993; 32:1361-1370. 
2. Chen H, Tan RX, Liu ZL, Zhang Y. Antibacterial Neoclerodane Diterpenoids fromAjuga lupulina, J. Nat. Prod.  1996; 59:668-670.
3. Konoshima T, Takasaki M, Tokuda H, Nishino H, Cancer chemopreventive activity of an iridoid glycoside, 8-acetylharpagide, fromAjuga decumbens. Cancer Lett. 2000;157:87-92.
4. Kutepova TA, Syrov VN, Khushbaktova ZA, Saatov Z. Hypoglycemic activity of the total ecdysteroid extract from Ajuga turkestanica. Pharm. Chem. J.2001;35:608-609.
5. Breschi MC, Martmotti E, Catalano S, Flamini G, Morelli I, Pagni AM. Vasoconstrictor activity of 8-0-acetylharpagide from ajuga reptans.  J. Nat. Prcd. 1992;55:1145-1148.
6. Kaithwas G, Gautam R, Jachak SM, Saklani A. Antiarthritic effects of Ajuga bracteosa Wall ex Benth. in acute and chronic models of arthritis in albino rats, Asian Pac J Trop Biomed. 2012;2:185-188.
7. Hsieh WT, Liu YT, Lin WC. Anti-inflammatory properties of Ajuga bracteosa in vivo and in vitro study and their effects on mouse model of liver fibrosis. J. Ethnopharmacol, 2011;135:116-125.
8. Akbay P, Gertsch J, Calis I, Heilmann J, Zerbe O, Sticher O. Novel Antileukemic Sterol Glycosides fromAjuga salicifolia, Helv. Chim. Acta.2002; 85:1930-1942.
9. Chiou CT, Kuo YH, Chan YY, Juang SH, Chan HH, Wu TS. Ajugalide-B (ATMA) is an anoikis-inducing agent fromAjuga taiwanensis with antiproliferative activity against tumor cellsin vitro.  Phytochem. 2012; 80: 64–69.
10. Guo P, Li Y, Xu J, Guo Y, Jin DQ, Gao J, Hou W, Zhang T. Neo-Clerodane diterpenes fromAjuga ciliataBunge and their neuroprotective activities. Fitoterapia 2011;82:1123-1127.
11. Cocquyt K, Cos P, Herdewijn P, Maes L. Van den Steen P E, Laekeman G, Ajuga remota Benth: From  ethnopharmacology  to  phytomedical  perspective  in the  treatment  of  malaria, Phytomed.2011;18:1229-1237.
12. Ben Jannet H, H-Skhiri F, Mighri Z, Simmonds MSJ, Blaney WM. Antifeedant activity of plant extracts and of new natural diglyceride compounds isolated fromAjuga pseudoialeaves againstSpodoptera littoralislarvae, Ind. Crop. Prod. 2001; 14:213-222.
13. Maggi F, Cecchini C, Cresci A, Coman MM, Tirillini B, Sagratini G, Papa F, Vittori S. Chemical Composition and Antimicrobial Activity of the Essential Oils from Several Hypericum Taxa (Guttiferae) Growing in Central Italy (Appennino Umbro-Marchigiano). Chem. Biodivers. 2010;7:447-466.
14. Li WJ, Nie SP, Liu XZ, Zhang H, Yang Y, Yu Q, Xie MY. Antimicrobial properties, antioxidant activity and cytotoxicity of ethanol-soluble acidic components from Ganoderma atrum. Food. Chem. Toxicol.  2012; 50: 689–694.
15. Ye CL, Dai DH, Hu WL. Antimicrobial and antioxidant activities of the essential oil from onion (Allium cepaL.) Food Control. 2013;30:48-53.
16. Ebrahimabadi AH, Djafari-Bidgoli Z, Mazoochi A, Jookar Kashi F, Batooli H. Essential oils composition, antioxidant and antimicrobial activity of the leaves and flowers of Chaerophyllum macropodum Boiss. Food Control.  2010;21:1173-1178.
17. Sökmen A, Vardar-Ünlü G, Polissiou M, Daferera D, Sökmen M, Dönmez E. Antimicrobial Activity of
Essential Oil and Methanol Extracts of Achillea sintenisii Hub. Mor. (Asteraceae). Phytother. Res. 2003;17:1005-1010.
18. Casetti F, Bartelke S, Biehler K, Augustin M, Schempp CM, Frank U. Antimicrobial Activity Against Bacteria with Dermatological Relevance and Skin Tolerance of the Essential Oil from Coriandrum sativum L. Fruits Phytother. Res. 2012; 26: 420–424.
19. Anonymous. European pharmacopoeia (3rd ed.). Strasburg, France: Council of Europe, 1996. pp.121-122.
20. Adams RP, Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. Allured Publishing Co., Carol Stream, IL, USA, 2001
21. Wayne, PA, National Committee for Clinical Laboratory Standard. In Performance standards for antimicrobial disk susceptibility test. Sixth approved standard. M2-A6,1997.
22. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke, R H. Manual of clinical microbiology (7th ed., pp. 1773). Washington, DC: ASM. 1995.
23. Gulluce M, Sokmen M., Sahin F, Sokmen A, Adiguzel A. Ozer H. Biological activities of the essential oil and methanolic extract ofMicromeria fruticosa (L) Druce ssp. Serpyllifolia (Bieb) PH Davis plants from the eastern Anatolia region of Turkey. J. Sci. Food Agric. 2004;84:735-741.
24. Hosseini MM. Pazoki A. Zamani HA.Akhlaghi H. Chemical Composition of the Essential Oil from Aerial Parts of Ajuga chamaecistus Ging. subsp. Scopria in Brackish Regions of Iran. Jeobp. 2011;14:101-105.