Priming Effect of on the Enhancement of Germination Traits in Aged Seeds of Chamomile (Matricaria chamomilla L.) Seeds Preserved in Medium and Long-term Storage

Authors

1 Department of Agronomy, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Research Institute of Forests and Rangelands, Agricultural Research Education and extension Organization (AREEO), Tehran, Iran

Abstract

Chamomile (Matricaria chamomilla L.) is a widely used medicinal plant possessing several pharmacological effects due to presence of active compounds. In order to study of seed priming effects on seedling growth of chamomile, an experimental design, based on randomized complete design with three replications was conducted under greenhouse conditions in Research Institute of Forests and Rangelands in 2014-2015. Experimental factors were A) three chamomile accessions as code of gene bank 8959 (Brojen), 15123 (Arak) and 23879 (Isfahan), B) five conservation methods including: medium-term storage (active cold room 4 °C for 15 years), long- term storage (basic cold room-18 °C for 15 years), regenerated seeds in open storage 22 °C for 2 years (Control) and aged seed under accelerated ageing )40 °C,98% of relative humidity for 48 and 72h) and C) priming treatments including: without priming /(control), osmopriming (PEG-0.3Mpa), hormonal priming (Gibberllic acid 250 and 500ppm), hydropriming (imbibition with distilled water). Data collected for seed emergence percent, root and shoot length, seedling length, vigor index, seedling weight and three Proxidase, Catalase and Super Oxid Desmotaz (SOD) enzymatic activities. Variance analysis showed significant effects of all factors and their interactions except accession by conservation interaction effects for seedling length (P<0.01). Result showed that that accession two accessions 23789 (Esfahan) and15123 (Arak had higher mean values than one other accessions for most of the traits in both medium-term and long- term storage. For aged seeds, the higher root length, seedling length mean values were obtained in 48h accelerate aging test. In comparisons between priming treatments, the higher means of traits were obtained in osmopriming except germination percent. For enzymes activity the trends of accession were not similar but the effect of osmopriming on catalase activity were high and stable in three accessions. The result of priming by conservation interaction showed that the higher seed emergence was obtained in regenerated seeds (control). The higher root length was obtained in accelerated aging test by application of all of priming treatments except hydropriming. For vigor index, hormonal priming (GA250) had higher effect followed by osmopriming. Similarly, for enzymatic activities GA500 had significant impact on proxidas and catalase. It was proved that two priming technique Osmo-priming using (PEG) and hormonal priming (Gibberllic acid) were effective method for recovery of aged seed.

Keywords

References

1. Ghassemi Dehkordi N, Aslani A, Gordanpour N. Optimization and development ofchamomil drop formulation. Pajouhesh &Sazandegi. 2007;75:146-51.
2. Burlando B, Uerottal L, Cornara L, Bottini-Massa L. Herbal Principles in Cosmetics: Properties and Mechanisms of Action, CRC Press. 2010;109-112.
3. Seidler-Lozykowska K. Determination of the ploidy level in chamomile (Chamomilla recutita (L.) Rausch.) strains rich in α-bisabolol. J Applied Genetics. 2003;44:151-5.
4. Haghi G,  Hatami  A, Safaei A,  Mehran M. Analysis of phenolic compounds in Matricaria chamomilla and its extracts by UPLC-UV. Res Pharm Sci. 2014;9:31-37.
5. Darvishpadok A, Azemi M, Namjoyan F, Khodayar, M, Ahmadpour F, Panahi M. Effect of Matricaria chamomilla L. on blood glucose and glycosylated hemoglobin in female fertile diabetic rats. Res Pharm Sci. 2012;7:S19.
6. Grgesina D, Mandic M L, Karuzal L. Composition of Different Part of Matricaria Chamomilla, Prehrambeno. Tehnol. 1995;111-114.
7. Fennell C W, Light M E, Sparg SG, Stafford GI, Van Staden J. Assessing African medicinal plants for efficacy and safety: agricultural and storage practices. J Ethnopharmacol. 2004;95:113-121.
8. Food Agriculture Ogaization. Draft genebank standards for plant genetic resources for food and agriculture. http://www.fao.org/agriculture/crops/corethemes/theme/seeds-pgr/conservation/gbs/en/. 2013.
9. Royal Botanic Gardens Kew. Seed Information Database (SID). Version 7.1. http://data.kew.org/sid/. 2008.
10. Probert RJ. The role of temperature in the regulation of seed dormancy and germination. In Fenner M, editor. , ed., Seeds: The Ecology of Regeneration in Plant Communities, Ed 2 CAB International, Wallingford, UK. 2000;261-292.
11. Butler LH, Hay FR, Ellis RH, Smith RD, Murray TB. Priming and re-drying improve the survival of mature seeds of Digitalis purpurea during storage. Ann Bot. 2009;103:1261-1270.
12. Nawaz J, Hussain M, Jabbar A, Nadeem GA, Sajid M, Subtain M, Shabbir I. Seed Priming A Technique. Int J Agric Crop Sci. 2013;6:1373-1381
13. Imani AF, Sardoei AS, Shahdadneghah M. Effect of H2SO4 on seed germination and viability of Canna india L. ornamental plant. Int J Advanced Biologic Biomed. 2014;22:223-229.
14. MacDonald MB. Seed priming. (eds. M. Black and J. D. Bewley) Sheffield Academic Press. 2000;287-325.
15. Taylor AG, Allen PS, Bennett MA, Bradford KJ, Burrisand JS, Misra MK. Seed enhancements. Seed Sci. Res. 1998;8:245-256.
16. Harris D, Rashid A, Arif M, Yunas M. Alleviating micronutrient deficiencies in alkaline soils of the North-West Frontier Province of Pakistan: on-farm seed priming with zinc in wheat and chickpea. In: Andersen, Tuladhar P,Karki JK, Maskey KB. S.L. (Eds)  Micronutrients in South and South East Asia. Kathmandu: ICIMOD. 2005;143-151
17. Chiu KY, Sung JM. Effect of priming temperature on storability of primed sh-2sweet corn seed. Crop Sci. 2002;42:1996-2003.
18. Ansari O, CHoghazardi HR, Sharifzadeh F, Nazarli H. Seed reserve utilization and seedling growth of treated seeds of mountain rye (Secale mountanum) as affected by drought stress. Cercetari Agronomice in Moldova. 2012;2:43-48.
19. Lee SK, Kader, AA. Pre harvest and postharvest factors influencing vitamin C content of horticultural crops. Post Harv Biol Technol. 2000;20:207-220.
20. Maguire JD. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Sci. 1962;2:176-177
21. Abdual-baki AA, Anderson JD. Relationship between decarboxylation of glutamic acid and vigor in soybean seed. Crop Sci. 1973;13:222-226.
22. Elstner EF, Youngman R, Obwald W. Superoxide dismutase. In Bergmeyer J, Grabl BM (eds) Methods of Enzymatic Analysis vol. III. Enzymes oxidoreductases, 3rd ed. Weinheim: Verlag-Chemie. 1995;293-302 .
23. Omidbeygi  R. Approaches to processing of medicinal plants. Publications about the day, vol 1, 286p. and second Publication Designers, 424p. 1995. (In Persian).
24. Rincker, CM. Germination of forage crop seeds after 20 years of subfreezing storage. Crop Sci. 1983;23:229-231.
25. Priestley DA. Seed aging. Cornell University Press. 1986.
26. Alizadeh MA. Evaluation of percentage of germination, total speed of germination and vigor index of 17 medicinal plants species to ageing test. Proceeding of National Congress in Sustainable Development of Medicinal Plants, (NCSDMP), 27-29 July 2005 Mashhad. 2005;171-172.
27. Gheraghi F, Mahmoodi S, Jami M Parsa S. Seed germination and growth improvement in heracleum persicum Dest. By osmopriming. J Herb Drugs. 2012;2:229-238.
28. Tekrony DM. Accelerated aging. In: Van de venter, H.A. (Ed.) Seed vigor testing seminar. Copenhagen: ISTA. 1995;53-72.
29. Mac Donald MB. Seed deterioration: physiology, repair and assessment. Seed Sci Technol. 1999;27:177-237.
30. Marshal AH, Lewis DN. Influence of seed storage conditions on seedling emergence, seedling growth and dry matter production of temperate forage grasses. Seed Sci Tech.2004;32:493-501.
31. Janmohammadi M, Fallahnezhad Y, Golshan M, Mohammadi H. Controlled ageing for storability assessment and predicting seedling early growth of canola cultivars (Brassica napus L.). J Agric Biol Sci. 2008;3:22-26.
32. Amooaghaie R. The effect of hydro and osmopriming on alfalfa seed germination and antioxidant defenses under salt stress. African J Biotech. 2011;10:6269-6275.
33. Farooq M, Basra SMA, Tabassum R, Afzal I. Enhancing the performance of direct seeded fine rice by seed priming. Plant Prod Sci. 2006;9:446-456
34. Alivand R, Tavakol Afshari R, Sharifzadeh F. Effects of gibberellins, salicylic acid, and Ascorbic acid on improvement of germination characteristics on deteriorated seeds of Brassica napus. Iranian J Agric Sci. 2013;43:561-571.
35. Eisvand HR, Tavakkol-Afshari R, Sharifzadeh F, Madah Arefi H, Hesamzadeh Hejazi SM. Improvement of physiological quality of deteriorated tall wheat grass (Agropyron elongatum Host) seeds by hormonal priming for control and drought stress conditions. Int J Field Crop Sci. 2008;1:39.53-65. (In Persian).
36. Sajjadi Jaghoroghi SS, Alizadeh MA, Kalagari M. Effect of Osmopriming, Hydropriming and Pre-chilling on Seed Emergence Enhancement and Seedling Vigor of four Medicinal Species of Anthemis under Greenhouse Conditions, Bullet UASVM Hortic. 2014;71:74-84.
37. Arteca NR. Plant Growth Substances: Principles and Applications. Springer, Publication. 1995;146.
38. El-Araby M, Hegazi AZ. Response of tomato seed to hydro and osmopriming: and possible relation of some antioxidant enzymes and endogenous polyamine fractions. Egyptian J Biol. 2004;6:81-93.
39. Yao Z, Liu L, Gao F, Rampitsch C, Reinecke DM, Ozga JA, Ayele BT. Developmental and seed aging mediated regulation of antioxidative genes and differential expression of proteins during pre and post germinative pHases in pea. J Plant Physiol. 2012;169:1477-1488.
40. Azooz MM. Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes differing in salt tolerance. Int J Agric Biol. 2009;11:343-350.
41. Burguieres, E, McCu P, Kwon Y, Shetty K. Effect of vitamin C and folic acid on seed vigor response and phenolic-linked antioxidation activity. Biores Technol. 2007;98:1393-1404.
42. Yang XH, Wang YG, YW X, Wang HF, Ma JH. Effect of seed priming on physiological characteristics of soybean seedling under water stress. Chinese J Eco-Agric. 2009;17:1191-1195
43. Jie L, Gong She L, Dong Mei O, Fang Fang L, En Hua W. Effect of PEG on germination and active oxygen metabolism in wildrye (Leymu.7 chinensis) seeds. Acta Prataculturae Sinica. 2002;11:59-64.
Journal of Medicinal plants and By-product
Volume 6, Issue 1
April 2017
Pages 1-9

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