Comparison between Two Groups of Pathogenic Bacteria under Different Essential Oil Extract of Ocimum basilicum L.

Authors

1 Department of Biology, Jolfa Branch, Islamic Azad University, Jolfa, Iran

2 Department of Veterinary, College of Veterinary, Shabestar Branch, Islamic Azad University, Shabestar, Iran

Abstract

This study was conducted to assessment the antibacterial activities of different part of basil essential oil on the standard gram-negative bacteria include Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and gram-positive ones including Bacillus cereus, Staphylococcus aureus, and Listeria monocytogen. The basil essential oil was provided from two part of the plant (leaf and herb) at the two different developmental stages. The antibacterial properties of basil essential oil was studied on the standard bacteria by agar disk diffusion, then minimal inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were detected. The results of agar disk diffusion tests showed the inhibition zones as follow: Listeria monocytogen17.11-17.42 mm, St. aureus 29.20-30.56 mm, B. cereus 14.73-16.06 mm, E. coli 21.60-23.58 mm, Salmonella typhi 21.63-24.80 mm and for P. aeruginosa the maximum inhibition zones were observed on leaf essential oil. From the herb part of basil almost similar results were obtained: Listeria monocytogen 17.02-17.67 mm, St. aureus 29.60-30.41 mm, B. cereus 10.66-16.11 mm, E. coli 17.48-23.54 mm, Salmonella typhi 21.58-21.64 mm and for P. aeruginosa the maximum inhibition zones were observed. The MICs for gram-positive bacteria were as: B. cereus ranging 36-18 μg/mL, S. aureus 18 μg/mL, Listeria monocytogen 18-36 μg/mL and for gram-negative bacteria of E. coli, Salmonella typhi and P. aeruginosa were 18-9 μg/mL. Some of basil oil component increased by water stress. Amount of the main constituents of the oil such as linalool, methyl chavicol, 1,8–cineole and trans α-bergamotene significantly affected by water stress.

Keywords

References

1. Daneshian MA, Gurbuz B, Cosge B, Ipek A. Chemical Components of Essential Oils from Basil (Ocimum basilicum L.) Grown at Different Nitrogen Levels. Int J Nat Eng Sci. 2009;3:08-12.
2. Daneshian Moghaddam AM, Shayegh J, Mikaili P, Dolghari Sharaf J. Antimicrobial activity of essential oil extract of Ocimum basilicum L. leaves on a variety of pathogenic bacteria. J Med Plants Res. 2011;5:3453-3456.
3. Da-Silva F, Santos RHS, Diniz ER, Barbosa LCA, Casali VWD. De-Lima RR. Content and composition of basil essential oil at two different hours in the day and two seasons. Revista Brasileira de Plantas Medicinais. 2003;6:33-38.
4. Hussain AI, Anwar F, Sherazi STH, Przybylski R. Chemical composition, an antioxidant and antimicrobial activity of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem. 2008;108:986-995.
5. Omidbaigi R, Hassani A, Sefidkon F. Essential oil content and composition of sweet basil (Ocimum basilicum) at different irrigation regimes. J Essen Oil Bearing- plants. 2003;6:104-108.
6. Pandu Sastry K, Ramesh Kumar R, Niranjan Kumari A, Sneha G, Elizabeth M. Morpho-Chemical description and antimicrobial activity of different Ocimum species. J Plant Develop. 2012;19:53-64.
7. Ravid U, Putievsky E, Katzir I, Lewinsohn E. Enantiomeric composition of linalool in the essential oils of Ocimum species and in commercial basil oils. Flav & Frag J. 1997;12:293-296.
8. Politeo O, Jukic M, Milos M. Chemical composition and antioxidant capacity of free volatile aglycones from basil (Ocimum basilicum L.) compared with its essential oil. Food Chem. 2007;101:379-385.
9. Mazzanti G, Battinelli L, Salvatore G. Antimicrobial properties of the linalool-rich essential oil of Hyssopus officinalis L. var decumbens (Lamiaceae). Flav & Frag J. 1998;13:289-294.
10. Saha S, Sengupta Ch, Ghosh P. Biological activities of essential oils and methanol extracts of five Ocimum species against pathogenic bacteria. Czech J Food Sci. 2013;31:194-202.
11. Sajjadi SE. Analysis of the essential oils of two cultivated basil (Ocimum basilicum L.) from Iran. Daru. 2006;14:128-130.
12. Telci I, Bayram E, Yilmaz G, Avci B. Variability in essential oil composition of Turkish basils (Ocimum basilicum L.). Biochem Systematic Ecol. 2006;34:489-497. 
13. Lee SJ, Umano K, Shibamoto T, Lee KG. Identification of volatile components in basil (Ocimum basilicum L.) and thyme leaves (Thymus vulgaris L.) and their antioxidant properties. Food Chem. 2005;91:131-137.
14. Opalchenovaa G, Obreshkova D. Comparative studies on the activity of basil an essential oil from Ocimum basilicum L. against multidrug resistant clinical isolates of the genera Staphylococcus, Enterococcus and Pseudomonas by using different test methods. J Microbiol Methods. 2003;54:105-110.
15. Prasad G, Kumar A, Singh A.K, Bhattacharya A.K, Singh K, Sharma V.D. Antimicrobial activity of essential oils of some Ocimum species and clove oil. Fitoterapia. 1986;57:429-432.
16. Sinha GK, Gulati BC. Antibacterial and antifungal study of some essential oils and some of their constituents. Ind Perfum. 1990;34: 126-129.
17. Wannissorn B, Jarikasem S, Siriwangchai T, Thubthimthed S. Antibacterial properties of essential oils from Thaimedicinal plants. Fitoterapia. 1990;76:233-236.
18. Bernath J. Ecophysiological approach in the optimalization ofmedicinal plant, agro-systems. Herba Hungarica. 1990;29:7-15.
19. Dastouri MR, Fakhimzadeh K, Shayeg J, Dolgari Sharaf J, Valilou MR, Maheri Sis N. Evaluation antibacterial activity of the Iranian honey through MIC method on some dermal and intestinal pathogenic bacteria. J Animal & Veterinary Advances. 2008;7:409-412.
20. Adiguzel A, Gulluce M, Sengul M, Ogutcu H. Antimicrobial Effects of Ocimum basilicum (Labiatae) Extract. Turk J Biol. 2005;29:155-160.
Journal of Medicinal plants and By-product
Volume 6, Issue 2
September 2017
Pages 241-245

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