Investigation of Consecutive Separating Arrangements of Bio active Compounds from Black Tea (Camellia sinensis) Residue

Authors

1 Tea Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization, Lahijan, Guilan, Iran

2 Department of Medicinal Plants, Forests and Rangelands Research Institute; Agricultural Research, Education and Extension Organization, Tehran, Iran

Abstract

Every year lots of black tea (Camellia sinensis (L.) Kuntze) residue will produce in the factories. These residue are unusable whereas the bio active compounds can be extracted and used in the drag and food industries. Due to mentioned problems, this project was conducted years 2011 - 2012 with the aim to make a study on consecutive isolation of all bio active compounds from tea residue, that extraction of one compound won’t benefite to build a lateral products factory but isolation of all bio active compounds can increase productivity. In this survey, four compounds of caffeine, catechin, fiber and protein were be separated and measured from residue mixture in three steps from three sequential models. The isolation of caffeine and catechin were placed together in one step because the extraction condition was similar. Experiments was conducted with four replications and data was analyzed. The results indicated that effect of three sequence models was significant on extraction yield of caffeine, catechin, protein, and fiber (P<0/01). Comparison of yields indicated that the maximum amount of caffeine and protein was obtained from second sequence, also the maximum amount of catechin and fiber from third sequence. The economic comparison results among sequences indicated that the all sequences were economical however third sequence was introduced as the most economical model in terms ratio of benefit to cost due to high price of catechin and maximum rate.

Keywords


1. Parmer N, Rawat M, Kumar JV. Camellia Sinensis (Green Tea): A Review. Global J Pharmaco. 2012;6:52-59.

2. Tariq M, Naveed A, Barkat Ali K. The morphology, characteristics and medicinal properties of ‘Camellia sinensis’ tea. J Med Plants Research. 2010;4:2028-2033.

3. Sumpio BE, Cordova AC, Berke-Schlessel DW, Levites Y, Weinreb O, Maor G, Youdim MB, Qin F, Chen QH. Green tea, the Asian Paradox and cardiovascular disease. J Am Coll Surg. 2006;202:813-820.

4. Cabrera C, Gimenez R, Lopez MC. determination of tea components with antioxidant activity. J Agric Food Chemistry. 2003;51:4427-4435.

5. Cabrera C, Artacho R, Gimenez R. Beneficial effects of green tea-a review. J. Am Coll Nutrition. 2006;25:79-99.

6. Granja A, Pinheiro M, Reis S. Epigallocatechin gallate nanodelivery systems for cancer therapy. Nutrients. 2016;8:307.

7. Icen H, Guru M. Extraction of caffeine from tea stalk and fiber residues using super critical carbon dioxide. J Supercritical Fluids. 2009;50:225-228.

8. Marcel WL, Chi H. Pharmacological effects of green tea on the gastrointestinal system. European J Pharmaco. 2004;500:177-185.

9. Richard B, Denis G. Green tea: prevention and treatment of cancer by nutraceutical. Lancet. 2004;364:1021-1022.

10. Nie ShP, Xie MY. A review on the isolation and structure of tea polysaccharides and their bioactivities. Food Hydrocoll. 2011;25:144-149.

11. Wickremasinghe RL. Monographs on tea products in Sri Lanka. Tea research Institute of Sri Lanka. 1978;7:45-51.

12. Roofigari haghighat Sh, Shokrgozar S, Shirinfekr A, Azadi R, Serajie A, Cheraghi K, mohebbian S, Jalali M. Extraction of edible color from tea residue and its stability assessment. Tea Research Institute. Agricultural Research, Education and Extension Organization, Lahijan, Iran. 2017.

13. Guru M, Icen H. Obtaining of caffeine from tea fiber and stalk residues. Bioresource Technology. 2004;94:17-19.

14. Hara Y. Green Tea. Health benefits and applications. Japan, Tokyo: Food Techno Company. 2000.

15. Waterman PG, Mole S. Analysis of phenolic plant metabolites. Blackwell scientific publication, Oxford UK. 1994.

16. Liaquing S, Xiangyang W, Zhongyang W, Yuanfeng W. Studies on tea protein extraction using alkaline and enzyme methods. China: Gongshang University. 2007.

17. Emami, A. Protein Measurement Method. Journal of Plant Breeding Methods, Organization for Research, Education and Promotion of Agriculture, Water & Soil Res Inst. 1996;982:28.

18. Institute of Standards and Industrial Research of Iran. Cellulose measurement method. Standard number: 3394. 1992.

19. Sun Q, Tian Z. Studies of rice protein extraction using an alkaline method. Shiping Gongye Keji. 2003;24:38-40.

20. Gu L, Lu J, Ye B. Tea chemistry. Hefei: Chinese University of science and technology Publishing. 2002.

21. Soltani Gh, Najafi B, Turkmani c. Management of agricultural unit. Second Edition, Shiraz University Press, Shiraz. 1371.

22. Parsa F, Azadi R. Mohebbian S, Pedarpoor M. Sharifie R, Haghie A.Investigation and measurement of important component in residue of tea factories. Tea Research Institute. Agricultural Research, Education and Extension Organization, Lahijan, Iran. 2010.

23. McMurry J. Organic Chemistry 7th edition, Belmont: Thomson Learning. 2008.

24. Silberman, R. Isolating Caffeine from Tea. Ohio: Cengage Learning. Mason. 2008.

25. Wu J. Org. chem. Ohio: Cengage Learning. Mason. 2009.

26. Parsa F, Azadi R. Mogaddam Dorodkhani A. Investigation and measurement of important components in dust and three kinds of common residue in tea factory. Journal of Sciences and Technology of Agriculture and Natural Resources. 2008;12:243-252.

27. Heckman MA, Weil J, Gonzalez de Mejia E. Caffeine (1,3,7-trimethylxanthine) in foods: A comprehensive review on consumption, functionality, safety, and regulatory matters. J Food Sci. 2010;75:77-87.

28. Kyung HR, Yinzhe J. Recovery of catechin compounds from Korean tea by solvent extaction. Biores Technol. 2006;97:790-793.

29. Peterson J, Dwyer J, Bhagwat S, Haytowitz D, Holdon J, Eldridge AL, Beecher G, Aladesanmi J. Major flavonoids in dry tea. J food composition & Anal. 2005;18:487-501.

30. Sharma V, Gulati A, Ravindranath SD. Extracability of tea catechins as a function of manufacture procedure and temperature of infusion. Food chem. 2005;93:141-148.

31. Cheng A, Chen X, Wang W, Gong Z, Liu L. Contents of extractable and non-extractable polyphenols. Czech J. Food Sci. 2013;31:275-282.

32. Knight, I. and Monroe, J. Plasmid DNA. 1996.

33. Smiechowska M, Dmowski P. Crude fiber as a parameter in the quality evaluation of tea. Food Chemistry. 2006;94:366-368.