Study of Biochemical Compounds from Extract of Peel, Seed and Fruit Juice of some Pomegranate Cultivars (Punica granatum L.)

Authors

Department of Horticulture, Faculty of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Abstract

Pomegranate (Punica granatum L.) is a native plant, which has many different cultivars in Iran. This plant consists of rich biochemical compounds that plays an important role in human health. This research aims to study some of biochemical compounds of this valuable fruit. Extract of pell and seeds and juice of fruits were prepared. According to the outcomes of this study, there is a direct relationship between increasing amount of phenol compounds and the colour of fruit peel. This increase showed significant difference. The anthocyanin measurement in three cultivars showed that the colour of the peel and seeds of the fruit has a direct relationship with the amount of anthocyanin. Study of antioxidant activity with the method of DPPH revealed that the highest amount of antioxidant activity between the peel and the seeds of the three cultivars of pomegranate is dedicated to the peel of the BSY. The outcomes of the FRAP method showed that the highest amount of antioxidant activity among seeds and the skin belongs to the seed of the WSR. With the use of the ABTS method the highest amount of antioxidant activity belongs to the peel of the BSY cultivar. The results of the studying vitamin C revealed that the amount of Vitamin C per 100ml of the pomegranate juice is 54.6±2.2mg for RSQ, 42.8±2.3mg for WSR and 65.7±2.2mg for BSY. Analysing the biochemical compounds from different cultivars helps to sufficient selecting, defusing and commercializing the pomegranate.

Keywords


1. Awamleh H, Hassawi D, Migdadi H, Brake M. Molecular characterization of pomegranate (Punica granatum L.) landraces grown in Jordan using amplified fragment length polymorphism markers. Biotechnol. 2009;8:316-322.

2. Adhami VM, Khan N, Mukhtar H. Cancer chemoprevention by pomegranate: laboratory and clinical evidence. Nutr Cancer. 2009;61:811-815.

3. Dong L, Sui C, Liu Y, Yang Y, Wei J, Yang Y. Validation and application of reference genes for quantitative gene expression analyses in various tissues of Bupleurum chinense. Mol Biol Rep. 2011;38:5017-5023.

4. Bagri P, Ali M, Sultana S, Aeri V. New sterol esters from the flowers of Punica granatum L. J Asian Nat Prod Res. 2009;11:710-715.

5. Lansky E, Shubert S, Neeman I. Pharmacological and therapeutic properties of pomegranate. in Symposium on production, processing and marketing of pomegranate in the Mediterranean region: advances in research and technology. Séminaires Méditerranéens (CIHEAM). 2000;231-235

6. Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010;15:7313-7352.

7. Joye D, Luzio G. Process for selective extraction of pectins from plant material by differential pH. Carbohyd Polym. 2000;43:337-342.

8. Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent, in Methods Enzymol.1999;152-178.

9. Everette JD, Bryant QM, Abbey YA, Wangila GW, Walker RB. Thorough study of reactivity of various compound classes toward the Folin− Ciocalteu reagent. Journal of agricultural and food chemistry. 2010;58: 8139-8144.

10. Hara M, Oki K, Hoshino K, Kubio T. Enhancement of anthocyanin biosynthesis by sugar in radish (Raphanus sativus L.) hypocotyl. Plant Sci. 2003;164:259-265.

11. Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J. 2013;21:143-152.

12. Arts MJ, Sebastiaan J, Voss Guido HP, Aalt Bast H. A critical appraisal of the use of the antioxidant capacity (TEAC) assay in defining optimal antioxidant structures. Food Chem. 2003;80:409-414.

13. Wootton-Beard PC, Moran A, Ryan L. Stability of the total antioxidant capacity and total polyphenol content of 23 commercially available vegetable juices before and after in vitro digestion measured by FRAP, DPPH, ABTS and Folin–Ciocalteu methods. Food Res Int. 2011; 44:217-224.

14. Wang Q, Farrell G. All-fiber multimode-interference-based refractometer sensor: proposal and design. Opt Lett. 2006;31:317-319.

15. Arya S, Mahajan M, Jain P. Non-spectrophotometric methods for the determination of Vitamin C. Anal Chim Acta. 2000;417:1-14.

16. Brater M. The United States Pharmacopoeia (USP) 25 and National Formulary (NF) 20, Webcom Limited. 2002. Toronto.

17. Jimoh, FO, Adedapo AA, Aliero AA, Afolayan AJ. Polyphenolic Contents and Biological Activities of Rumex ecklonianus. Pharm Biol. 2008;46:333-340.

18. Naik GH, Priyadarsini KI, Satav JG, Banavaliker MM, Sohoni DP, Biyani MK, Mohan, H. Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochemistry. 2003;63:97-104.

19. Georgé S, Brat P, Alter P, Amiot MJ. Rapid determination of polyphenols and vitamin C in plant-derived products. J Agric Food Chem. 2005;53:1370-1373.

20. Kaur C, Kapoor HC. Anti‐oxidant activity and total phenolic content of some Asian vegetables. IJFST. 2002; 37:153-161.

21. Zarezadeh R, Emamjomeh Z, Shahedi M, Loni E, Akhavan HR, Biyabani J. Identification and quantification of anthocyanins in pomegranate peel extract. JFST. 2015;12:31-40

22. Alighourchi HR, Barzegar M, Sahari MA, Abbasi S. Effect of sonication on anthocyanins, total phenolic content, and antioxidant capacity of pomegranate juices. Int Food Res J. 2013;20:1703-1709.

23. Fawole OA, Opara UL. Effects of maturity status on biochemical content, polyphenol composition and antioxidant capacity of pomegranate fruit arils (cv.‘Bhagwa’). S Afr J Bot. 2013;85:23-31.

24. Tehranifar A, Selahvarzi Y, Kharrazi M, Jahanbakhsh V. High potential of agro-industrial by-products of pomegranate (Punica granatum L.) as the powerful antifungal and antioxidant substances. Ind Crop Prod. 2011;34:1523-1527.

25. Deng J, Cheng W, Yang G. A novel antioxidant activity index (AAU) for natural products using the DPPH assay. Food Chem. 2011;125:1430-1435.

26. Sharma OP, Bhat TK. DPPH antioxidant assay revisited. Food Chem. 2009;113:1202-1205.

27. Chen Z, Bertin R, Froldi G. EC50 estimation of antioxidant activity in DPPH assay using several statistical programs. Food Chem. 2013;138:414-420.

28. Mishra K, Ojha H, Chaudhury NK. Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food chem. 2012;130: 1036-1043.

29. Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem. 2005;53:4290-4302.

30. Roginsky V, Lissi EA. Review of methods to determine chain-breaking antioxidant activity in food. Food chem. 2005;92:235-254.

31. Pulido R, Bravo L, Saura-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem. 2000;48:3396-3402.

32. Ou B, Hampsch-Woodill M, Prior RL. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem. 2001;49:4619-4626.

33. Berker KI, Guclu K, Tor I, Apak, R. Comparative evaluation of Fe (III) reducing power-based antioxidant capacity assays in the presence of phenanthroline, batho-phenanthroline, tripyridyltriazine (FRAP), and ferricyanide reagents. Talanta. 2007;72:1157-1165.

34. Frankel EN, Meyer AS. The problems of using one‐dimensional methods to evaluate multifunctional food and biological antioxidants. J Sci Food Agr. 2000; 80:1925-1941.

35. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem. 2005; 53:1841-1856.