Callus Induction, Shoot and Root Regeneration in Hyssopus officinalis using Sodium Nitroprusside and Plant Growth Regulators

Sadat-Hosseini, Mohammad; Soleimani, Amanollah

doi:10.22034/jmpb.2023.363600.1611

Callus Induction, Shoot and Root Regeneration in Hyssopus officinalis using Sodium Nitroprusside and Plant Growth Regulators

Document Type : Research Paper

Authors

¹ Department of Horticultural Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran

² Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Jiroft, Jiroft, Iran

10.22034/jmpb.2023.363600.1611

Abstract

The current study aims to assess the callus induction and regeneration of shoots and roots in H. officinalis using sodium nitroprusside (SNP) and plant growth regulators (PGRs). A significant impact was found in the callogenesis of leaf and petiole explants with different concentrations of SNP and 2,4-dichlorophenoxyacetic acid (2,4-D). SNP concentrations of 20 µm induced callogenesis to 72%, but when combined with 2,4-D concentrations, callogenesis increased to 100%. In the absence of SNP, no calluses were developed. A significant effect of 2,4-D and SNP was observed on callus fresh weight. Callus fresh weight from leaf explants was 0.165 g, while from petiole explants was 0.13 g. Furthermore, SNP enhanced shoot regeneration of leaf explants compared to petiole explants. When 20 µm of SNP was combined with 3 mg/L 6-benzylaminopurine (BAP), the maximum mean shoot number was obtained (84% in leaves and 54% in petioles). A combination of SNP and IBA enhanced rooting rate to 83% and root length to 8.3 cm. After acclimation, over 80% of the produced plants survived in greenhouse conditions. Several implications can be drawn from the study's findings regarding medicinal hyssop cultivation, the preservation of genetic resources, and pharmaceutical research on medicinal plants.

Keywords