Document Type : Research Paper
Authors
1
Graduated of Veterinary Medicine, Faculty of veterinary medicine, Science and research branch, Islamic azad university, Tehran, Iran
2
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3
Department of Biology and Anatomical Sciences, School of medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4
Department of clinical sciences, Faculty of veterinary medicine, Science and research branch, Islamic azad university, Tehran, Iran
5
Department of pathology, Faculty of veterinary medicine, Science and research branch, Islamic azad university, Tehran, Iran
Abstract
Objective: This study aimed to investigate the effects of Salvia Officinalis extract on infertility of male rats.
Material and methods: In this experimental study, 40 rats were used in 8 groups and cadmium chloride was also used to induce infertility. Salvia Officinalis extract was fed to mice daily for 28 days with the help of gavage needle. Animals were anesthetized with ether and dissected, then epididymal sperm were examined for morphology, viability and motility. we analyzed their right testis for H/E staining and counting cell lines and their left testicles were analyzed to evaluate superoxide dismutase (SOD) and malondialdehyde (MDA) enzymes.
Results: According to the results of three sperm quality analyses indicated a significant increasing of viability, motility and the number of sperm cells in infertile groups treated with 100, 200, and 400 mg/kg Salvia Officinalis extract (P<0.001). Our SOD analysis revealed Salvia Officinalis extract caused to produce more enzymes in the healthy experimental groups, even compared to the healthy control group (P<0.0001). our analysis indicated that increasing the dosage of the Salvia Officinalis extract resulted in increasing enzyme production in infertile groups. MDA analyses showed that the healthy experimental groups produced a lower amount of MDA enzyme, which is almost the same level as the healthy control group (P<0.0001). due to the usage of Salvia Officinalis extract, the level of lipid peroxidation and MDA significantly decreased.
Conclusion: In this study, Salvia officinalis extract can have significant positive impacts on the viability, motility, number of sperms in oxidative stress conditions.
Keywords
Main Subjects
)