%0 Journal Article %T Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus) %J Journal of Medicinal plants and By-product %I Iranian Medicinal Plants Society %Z 2322-1399 %A Hassanloo, Thahereh %A Jafarkhani Kermani, Maryam %A Malmir Chegini, Mahsa %A Sepehrifar, Roshanak %A Mohajeri Naraghi, Sepehr %A Miri, Seied Mehdy %D 2014 %\ 09/01/2014 %V 3 %N 2 %P 199-205 %! Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus) %K Medicinal plants %K Micropropagation %K in vitro %K growth regulators %K Vaccinium arctostaphylus %R 10.22092/jmpb.2014.108734 %X In order to optimize the micropropagation of Qare-Qat (Vaccinium arctostaphylus J.J.Sm), samples were collected from two regions of Iran (Asalem and Kelardasht). Anderson (AN) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg L-1) and different levels of pH (4.5, 5 and 5.5) were compared. The in vitro shoots were transferred on AN media supplemented with zeatin (1 and 2 mg L-1) alone or in combination with Indole-3-butyric acid (IBA) (0.2 and 0.4 mg L-1). To optimize the rooting stage, elongated shoots (1.5- 2cm) were cultured on half strength semi- solid media containing activated charcoal (0 and 7 g L-1),or half strength liquid AN medium supplemented with different concentrations of IBA (0, 0.5, 2 and 3 mg L-1) and NAA (0 and 0.5 mg L-1). The results indicated that at the establishment stage AN media supplemented with 4 mg L-1 zeatin (pH= 5.5) and 1 mg L-1 zeatin (pH= 5) were the best treatment for Asalem and Kelardasht explants, respectively. For the Asalem explants, the highest number of proliferated shoots (4) was observed on AN media consisting of 2 mg L-1 zeatin and 0.4 mg L-1 IBA. Whereas for the Kelardash explants  the highest number of proliferated shoots (3) was observed on AN media consisting of 2 mg L-1 zeatin and 0.2 mg L-1 IBA. The highest percent of root formation (86 and 66%) were observed in half strength AN medium containing of 7 g L-1 activated charcoal and 3 mg L-1 IBA and 0.5 mg L-1 NAA in Asalem and Kelardasht explants, respectively. The plantlets were successfully transferred to soil and the survival rate was 85%. %U https://jmpb.areeo.ac.ir/article_108734_828fd2857a7b7b4788487da60b5d9723.pdf