TY - JOUR ID - 124367 TI - Comparison of Essential Oils Composition Between in-vitro Plantlets and Greenhouse Plants from Various Populations of Dracocephalum kotschyi Boiss JO - Journal of Medicinal plants and By-product JA - JMPB LA - en SN - 2322-1399 AU - Allahverdi-Mamaghani, Bahareh AU - Hesamzadeh Hejazi, Seyed Mohsen AU - Mirza, Mehdi AU - Movafeghi, Ali AD - Faculty of Natural Sciences, Tabriz University, Tabriz, Iran AD - Research Institutes of Forests and Rangelands, Department of Biotechnology, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran AD - Medicinal and Aromatic Plants Division, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran Y1 - 2022 PY - 2022 VL - 11 IS - 2 SP - 219 EP - 230 KW - Dracocephalum kotschyi Boiss KW - Gas chromatography KW - Tissue culture KW - Methyl geranate KW - Neral KW - Verbenone KW - geranyl acetate DO - 10.22092/jmpb.2021.353864.1343 N2 - Dracocephalum kotschyi Boiss. belongs to the family Lamiaceae, a perennial herbaceous medicinal plant that is native to Iran and is considered an endangered species. In-vitro plantlets (seven populations) were raised in MS medium supplemented with 0.1 mg/l BAP and 0.01 mg/l NAA and the rooted plantlets were acclimatized successfully under greenhouse conditions. In- vivo plants (eight populations) were propagated under greenhouse condition. The essential oils were isolated by hydro distillation and identification of chemical compounds was done by a combination of capillary GC and GC-MS instruments. Twenty-five and forty compounds were identified in the different populations of in-vitro plantlet and in-vivo plant constituting 85.8%-99.68% and 85.1%-95.06% of essential oils, respectively. The major components of in-vitro plantlet on different populations were Verbenone (2.5%-82.47%), Geranyl acetate (28.35%-62.07%), Methyl geranate (0.98%-79.06%), Neral (0.64%-3.13%), Geranial (4.93%-14.39%), Limonene (0.37%-10.36%) and E-Anethole (7.62%-20.71%). The composition of essential oil from greenhouse plant populations were dominated by Neral (11.24%-74.80%), Limonene (0.17%-25.26%), Geraniol (0.54%-40.81%), Geranial (0.04%-9.15%), Methyl geranate (0.16%-28.48%), E-Anethole (0 %-0.1%) and Verbenone (0.39%-23.96%). The highest values of Neral, Limonene and Geraniol percentage were obtained from greenhouse conditions. In contrast, the maximum values of Verbenone, Geranyl acetate, Methyl geranate, Geranial, and E-Anethole were observed in the essential oils of in-vitro plantlets. This study demonstrated difference of chemical composition between in-vitro plantlets and greenhouse plants of different populations on D. kotschyi species. Also, new chemotypes of D. kotschyi has been introduced for further research. UR - https://jmpb.areeo.ac.ir/article_124367.html L1 - https://jmpb.areeo.ac.ir/article_124367_49a59c420d8379f3bc94072875d21746.pdf ER -