Iranian Medicinal Plants Society Journal of Medicinal plants and By-products 2322-1399 2 1 2013 04 01 Influence of Methyl Jasmonate on Menthol Production and Gene Expression in Peppermint (Mentha x piperita L.) 75 82 108494 10.22092/jmpb.2013.108494 EN Soheila Afkar Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, P. O. Box 14115-336, Iran Ghasem Karimzadeh Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, P. O. Box 14115-336, Iran Mokhtar Jalali-Javaran Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, P. O. Box 14115-336, Iran Mozafar Sharifi Plant Biology Department, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran Mehrdad Behmanesh Plant Biology Department, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran Journal Article 2013 02 05 Peppermint has considerable commercial value and widely cultivated for essential oil production, especially menthol. The aim of this study was to determine the quantitative expression of pulegone reductase (<em>pr</em>), menthofuran synthase (<em>mfs</em>) and limonene synthase (<em>ls</em>) genes in menthol biosynthesis pathway in <em>Mentha </em>x <em>piperita </em>, using semiquantitative RT-PCR analysis and evaluating menthol production by GC/MS analysis in presence of different methyl jasmonate concentrations (MJ; 0, 0.1, 0.5 mM). RT-PCR analysis showed that <em>pr</em>, <em>mfs</em> and <em>ls</em> specifically induced by MJ treatment. The transcripts of these genes up-regulated within 4-12 h of MJ induction followed by down-regulation after 24-48 h of MJ exposure. https://jmpb.areeo.ac.ir/article_108494_5069d91ef48b8668e8d6bfd7c6fa0c94.pdf