Iranian Medicinal Plants Society Journal of Medicinal plants and By-products 2322-1399 15 6 2026 11 01 Anti-Catalyze Enzyme, Anti-biofilm, Antibacterial Effectiveness of Calcium Bio-Synthesized Nanoparticles from Pseudomonas aeruginosa 698 704 135394 10.22034/jmpb.2026.372377.2159 EN Ismail Jmia Abbass Department of Biology, College of Education, Quran, University of Basrah, Basrah, Iraq Journal Article 2026 02 23 Nanotechnology is a crucial modern science focused on the creation of small-sized particles with significant effectiveness, particularly in health applications. There are three primary methods for synthesizing nanoparticles: physical, chemical, and biological. The biological method is preferred due to its environmentally friendly nature, low cost, and minimal equipment requirements. This study utilized the biological method to synthesize calcium nanoparticles (CaNPs) using the supernatant solution from Pseudomonas aeruginosa bacteria isolated from soil. The nanoparticles were characterized microscopically, morphologically, and molecularly. Characterization techniques included colorimetric analysis, UV-visible spectrophotometry, FT-IR, and scanning electron microscopy (SEM), all of which confirmed the successful synthesis of CaNPs. Biofilm-forming bacteria were identified using Congo red and microtiter plate methods, revealing that the pathogenic isolates Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus exhibited a strong ability to form biofilms. Additionally, the effectiveness of the synthesized nanoparticles in inhibiting biofilms formed by these pathogenic bacteria was tested, demonstrating their antibacterial properties and ability to inhibit catalase enzyme activity.

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