β-cyclocitral Extracted from Gallium Mite (medicinal herb) and the Evaluation of its Effect in MCF-7 Cell Lines

Shabani, Alireza; Zaefizadeh, Mohammad; Farhadpour, Mohsen; Soheila Soheili, Zahra; Esfahani, Kasra

doi:10.22034/jmpb.2024.362994.1585

β-cyclocitral Extracted from Gallium Mite (medicinal herb) and the Evaluation of its Effect in MCF-7 Cell Lines

Document Type : Research Paper

Authors

¹ Department of Plant Molecular Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

² Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran

10.22034/jmpb.2024.362994.1585

Abstract

Carotenoids play important role as antioxidant and induction of gene expression changes lead to plants acclimation to environmental stresses. Βetacyclocitral (BCC) derived from Gallium mite in the process of β-carotene oxidation has recently proved that has a new function in breast cancer treatment. In this project we used drug loaded delivery released system (DDRS) strategy, coupled with super paramagnetite nanoparticles (SNPs) based on graphene quantum dots (GQDs) synthesis to deliver drugs to tumor sites. The validation of synthesized SNPs was implemented with several techniques such as Fourier-transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM). MCF-7 cell lines were treated with GQD, β-cyclocitral and GQD@β-cyclocitral (each with concentration of 3.93, 7.87, 15.75, 31.25, 62.5, 125, 250, 500, and 1000 µg/ml) in MTT assay in 24 and 48 hours respectively. IC50% was calculated in 24 h. Results indicated that β-cyclocitral had prominent effects in breast cancer cell lines controlling.

Keywords