Effect of Vitamin B Complex on some Biochemical Parameters of Aloe vera


Nickel and cadmium are heavy metals with some hazards for plant metabolism. The impacts of nickel chloride (0, 400 and 800 μM) in the presence of vitamin B complex (0, 30 and 60 ml from pharmacy commercial stock) and the impacts of cadmium chloride (0, 100, 200, 300, 400, 500 and 600 μl from 100 µM stock) in pot and cadmium nitrate (0, 100, 200, 400, 800, 1600 and 3200 μl from 100 µM stock) in pot on fresh weight, photosynthetic pigments including chlorophyll and carotenoid, total protein and free proline content of Aloe vera L. seedlings were investigated. The inhibitory effects of nickel and cadmium on seedling growth resulted in decrease of chlorophyll and total protein contents. Free proline increased, while the fresh weight of the seedling was not affected by the treatments. The results indicate that vitamin B complex alleviated the inhibitory effects of nickel on A. vera L. seedlings by increasing chlorophyll, fresh weight and total protein contents.


1. Cheesbrough M. Medical Laboratory Manual for Tropical Countries. Oxford, London; 2000.

 2. Gordon MC, David JN. Natural product drug discovery in the next millennium. Pharm Biol. 2001;39:8-17.

3.Pál1 M, Horváth E, Janda T, Páldi E, Szalai G. Physiological changes and defense mechanisms induced by cadmium stress in maize. J Plant Nutr Soil Sci.2006;169:239–246.

4. Salt DE, Kato N, Kramer U, Smith RD, Raskin I. The role of root exudates in nickel hyperaccumulation and tolerance in accumulator and nonaccumulator species of Thlaspi. In: Terry N, Banuelos G (eds.) Phytoremediation of contaminated soil and water. CRC Press, London; 2000. pp.189–200.

5. John R, Ahmad P, Gadgil K, Sharma S. Heavy metal toxicity: Effect on plant growth, biochemical parameters and metal accumulation by Brassica juncea L. Int J Plant Prod. 2009;3:65-75.

6. Agar G, Taspinar MS. Effect of calcium, selenium and zinc on cadmium induced chromosomal aberration   in   root   of   Secale   cereale.   Fresen Environ Bul. 2003;12:1471-1475.

7. Surjendu K, Jayashree D, Sanjukta P, Debasmite P. Changes in the  antioxidative enzyme activities and lipid peroxidation in wheat seedlings exposed  to cadmium and lead stress. Braz J Plant Physiol. 2007;19:219-229.

8. Shahrtash M,Mohsenzadeh S, Mohabatkar H. Cadmium-induced genotoxicity detected by the random amplification of polymorphism DNA in the maize seedling roots. J Cell Mol Res. 2010;2:42-48.

9. Havaux M, Ksas B, Szewczyk A, Rumeau D, Franck F, Caffarri S,   Triantaphylidès C. Vitamin B6 deficient plants display increased sensitivity to high light and photo-oxidative stress. BMC Plant Biol. 2009;9:130-152.

10. Mooney S, Leuendorf JE, Hendrickson C, Hellmann H. Vitamin B6: a long known compound of surprising complexity. Molecules. 2009;14:329-351.

11. Tambasco-Studart M, Titiz O, Raschle T, Forster G, Amrhein N, Fit-zpatrick TB. Vitamin B6 biosynthesis in higher plants. Proc Natl Acad Sci USA. 2005;102:13687-13692.

12. Denslow SA, Walls AA, Daub ME. Regulation of biosynthetic genes and antioxidant properties of vitamin B6 vitamers during plant defense responses.  Physiol Mol Plant Pathol. 2005;66:244-255.

13. Lichtenthaler HK. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol. 1987;148:350-382.

14. Bates LS. Rapid determination of free proline for water stress studies. Plant Soil. 1973;39:205-207.

15. Bradford M. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochem. 1976;72:248-254.

16. Mohsenzadeh S, Shahrtash M, Mohabatkar H. Interactive effects of salicylic acid and silicon on some physiological responses of cadmium-stressed maize seedlings. Iranian J Sci Technol. 201;1:57-60.

17. Liang Y, Wong JWC, Wei l. Silicon mediated enhancement of cadmium tolerance in maize (Zea mays L.) grown in contaminated soil. Chemosphere. 2005;58:475-483.

18. Clemens S. Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie.2006;88:1707-1719.

19. Seregin IV, Kozhevnikova AD. Physiological role of nickel and its toxic effects on higher plants. Russian J Plant Physiol. 2006;53:257-277.

20. Pandey N, Sharma CP. Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage. Plant Sci. 2002;163:753-758.

21. Ahmad MSA, Hussain M, Saddiq R, Alvi AK. A nickel indicator, accumulator or excluder? Bull Environ Contam Toxicol. 2007;78:319-324.

22. Zengin FK, Munzuroglu O. Effects of some heavy metals on content of chlorophyll, proline and some antioxidant chemicals in bean (Phaseolus vulgaris L.) seedlings. Acta Biol Cracov Bot. 2005;47:157-164.

23. Michalak A. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol J Environ Stud.2006;15:523-530.

24. Boisvert S, Joly D, Leclerc S, Govindachary S, Harnois J, Carpentier R. Inhibition of the oxygen-evolving complex of photosystem II and depletion of extrinsic polypeptides by nickel. BioMetals. 2007;20:879-889.

25. Gajewska E. Sklodowska M, Slaba M, Mazur J. Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots. Biol Plant. 2006;50:653-659.

26. Howitt CA, Pogson BJ. Carotenoid accumulation and function in seeds and non-green tissues. Plant Cell Environ.2006;29:435-445.

27. Choudhary M, Jetley UK, Khan MA, Zutshi S, Fatma T. Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5. Ecotox Environ Safety. 2007;66:204-209.

28. Ramon O, Vazquez E, Fernandez M, Felipe M, Zornoza P. Cadmium-stress in white lupine: effects on nodule structure and functioning. Plant Physiol Bioch. 2003;161:911-919.

29. Ahmad P, John R. Effect of Salt stress on growth and biochemical parameters of Pisum sativum L. Arch Agro Soil Sci. 2005;51:665-672.

30. Helmy LH. The influence of nickel sulphate on some physiological aspects of two cultivars of Raphanus sativus L. Arch Biol Sci Belgrade. 2010;62:685-693.

31. Singh S, Sinha S. Accumulation of metals and its effects in Brassica juncea (L.) Czern. (cv. Rohini) grown on various amendments of tannery waste. Ecotoxicol Environ Safety. 2005;62:118-127.

32. Palma JM, Sandalio LM, Corpas FJ, Romero-Puertas MC, McCarthy I, Del Rio LA. Plant proteases, protein degradation and oxidative stress: Role of peroxisomes. Plant Physiol Bioch. 2002;40:521-530.

33. Mohsenzadeh S, Shahrtash M, Teixeira da Silva JA. Silicon improves growth and alleviates toxicity of cadmium in maize seedlings. Plant Stress. 2012;6:39-43.

34. Gajewska E, Sklodowska M, Slaba  M, Mazur J. Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots. Biol Plant. 2006;50:653-659.

35. Gajewska E, Sklodowska M. Effect of nickel on ROS content and antioxidative enzyme activities in wheat leaves. BioMetals 2007;20:27–36.

36. Gajewska E, Sklodowska M. Antioxidative responses and proline level in leaves and roots of pea plants subjected to nickel stress. Acta Physiol Plant. 2005;27:329-339.

37. Zhao J, Shi G, Yuan Q. Polyamines content and physiological and biochemical responses to ladder concentration of nickel stress in Hydrocharis dubia (Bl.) Backer leaves. BioMetals. 2008;21:665-674.