Ecological Requirements, Antioxidant Activity and New Chemotype Essential Oil from Achillea millefolium L. and Achillea micrantha Wild. in North of Iran (Golestan Province)

Authors

1 Department of Botany, Gorgan Branch, Islamic Azad University, Gorgan, Iran

2 Department of Agriculture, Astara Branch, Islamic Azad University, Astara, Iran

3 Pharmacist, Niak Pharmaceutical Lab, Gorgan, Iran

Abstract

In many field observation, ecological equipment, phenology and ethnopharmacological data of Achillea millefolium L. and A. micrantha Wild. were studied. The inflorescences of plants were collected in different locations of Golestan province: Chaharbagh (2000 m) and Dozan (2200 m) respectively. Essential oils were obtained by steam distillation (Clevenger) and analyzed by gas chromatography-mass spectrometry and (GC-MS). Total phenol (TP) and total flavonoid (TF) were determined with spectro photo meteri. Antioxidant properties were obtained by three radical scavenging activity methods: reducing power (RP), total antioxidant capacity (TAC) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH). Field observation showed that both species of Achillea are perennial aromatic plants which can grow 30 to 70 cm tall, flowers appeared in mid to late of May where the annual average rainfall were 399-345 mm, in sandy loam to silty clay loam soil. These plants has been used in traditional medicine as antibacterial, anti infection ,anti spasm, sedative, astringent, anti nociceptive and wound healing. Flowers essential oils of A. millefoilum and A. micrantha were characterized by higher amounts of similar constituents: binapacryle (63.82%ˏ 83.63%), 1-8 cineol (14.97%ˏ 3.76%) and α-selinene (4.81%ˏ 4.49%) respectively. TP content (12.34±0.264 to 18.44±0.085 mgGAEgr-1)and TF contents (61.003±2.38 to ‎80.30±5.793 mgQUE g-1) were measured in A. micrantha and A. millefolium, respectively. A. micrantha had more antioxidant activity with IC50 (0.184±0.0475 ‎µg/ml in dry weight in DPPH method) than A. millefolium (IC50 ‎0.178±0.178 µg/ml in dry weight in RP method). According to the results, there is a positive correlation between antioxidant activity and important secondary metabolites (TP, TF), this could help to study more about the application of these plants in traditional medicine as an antiseptic, anti spasm and antibacterial agent.

Keywords

References

1. Bimbiriati K, Ragažinskienė O, Maruška A, Kornyšova1 O. Comparison of the chemical composition of four yarrow (Achillea millefolium L.) morphotypes. Biologia 2008;54:208-212.
2. Kırca A, Arslan E. Antioxidant capacity and total phenolic content of selected plants from Turkey. Food Sci Technol Int J. 2008;43:2038‐2046.
3. Paduch R, Matysik G, Nowak-Kryska M, Niedziela P, Kandefer-Szerszen M. Essential oil composition and in vitro biological activity of Achillea millefolium L. extracts. Pre-clin and Clin Res J. 2008;2:049-058.
4. Akkol EK, Koca U, Pesin I, Yilmazer D. Evaluation of the wound healing potential of Achillea biebersteinii Afan. (Asteraceae) by in vivo excision and incision models. Evid-Based Complement Alt Med. 2011; Vol 2011;7 pages.
5. Hemmati AA, Arzi A, Adinehvand A, Mostofi NE, Mozaffari AR, Jalali A. Yarrow (Achillea millefolium L.) extract impairs the fibrogenic effect of bleomycin in rat lung. Med Plants Res J. 2011;5:1843-1849.
6. Saeidnia S,Gphari AR, Mokhber-Dezfuli N, Kiuchi F. A review on phytochemistry and medicinal properties of the genus Achillea. Daru 2011;19:173-186.
7. Nemeth E. Essential oil composition of species in the genus Achillea. Essent Oil Res J. 2005;17:501-512.
8. Gudaityte O, Venskutonis PR. Chemotypes of Achillea millefolium transferred from 14 different locations in Lithuaniato the controlled environment. Biochem Syst Ecol. 2007;35:582–592.
9. Aghjani Z, Masoudi SH, Rustaiyan A. Composition of essential oil from flowers of Achillea tenuifolia Lam. J Essent Oil. 2000;12:723-724.
10. Anon A. Dictionary of Natural Products on CD-ROM, Ver.14.1 1982-2006, Chapman & Hall/CRC. 2006.
11. Bader A, Flamini G, Cioni PL, Morelli I. Essential oil composition of Achillea santolina L. and Achillea biebersteinii Afan. collected in Jordan. Flavour Fragr J. 2003;18:36-38.
12. Baris Z, Gulluce M, Sahin F, Ozer H, Kilic H, Ozkan H, Sokmen M, Azbek T. Biological Activities of the Essential Oil and Methanol Achillea biebersteinii Afan. (Asteraceae). Turkish J Biol. 2006;30:65-73.
13. Filippi JJ, Lanfranchi DA, Prado S. Composition, enantiomeric distribution, and antibacterial activity of the essential oil of Achillea ligustica All. from Corsica. J Agric Food Chem. 2006;54:6308-6313.
14. Rahimmalek M, Sayed Tabatabaei BE, Etemadi N, Goli SAH, Arzani A, Zeinali H. Essential oil variation among and within six Achillea species transferred from different ecological regions in Iran to the field conditions. Ind Crops Prod. 2009;29:348-355.
15. Tuberos CI, Kowalczyk A, Coroneo V, et al. Chemical composition and antioxidant, antimicrobial, and antifungal activities of the essential oil of Achillea ligustica. J Agric Food Chem. 2005;53:10148-10153.
16. Kessler M, Ubeaud G, Jung L. Antiand pro‐oxidant activity of rutin and quercetin derivatives. Pharm Pharmacol J. 2003;55:131‐142.
17. Surveswaran S, Cai YZ, Corke H, Sun M. Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chem. 2007;102:938‐953.
18. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol. 2006;5:1142-1145.
19. Eghdami A, Fateh V, Eradatmand asli D, Houshmandfar A. Antioxidant activity, total phenolic and flavonoids contents in methanolic and aqueous extract of Achillea millefolium L. Adv  Environ Biol. 2011;5:929-932.
20. Adams R. Identification of essentials oil components by Gas Chromatography/ Mass Spectroscopy. Allured Publishing Corp. Carol Stream, Illinois. 1995 pp. 1-456.
21. Özgen U, Mavi A, Terzi Z, Coflkun M, Yildirim A. ‎Antioxidant activity and total phenolic compounds amount of some ‎Asteraceae species. Turkish J Pharm Sci. 2004;1:203-216.‎
22. Arabshahi-Deloee S, Urooj A. Antioxidant propperties of various solvent extracts of mulberry (Morus indica L.) ‎leaves. Food Chem. 2006;102:1233-1240.‎
23. Demirci F, Demirci B, Gurbuz I, Yesilada E, Can Baser KH. Characterization and biological activity of Achillea teretifolia Willd. and A. nobilis L. subsp. neilreichii (Kerner) formanek essential oils. Turkish J Biol. 2009;33:129-136.
24. El-Shazly AM, Hafez SS, Wink M. Comparative study of the essential oils and extracts of Achillea fragrantissima (Forssk.) Sch. Bip. and Achillea santolina L. (Asteraceae) from Egypt. Pharmazie 2004;59:226-230.
25. Si XT, Zhang ML, Shi QW, Kiyota H. Chemical constituents of the plants in the genus Achillea. Chem Biod. 2006;3;1163-1179.
26. Tuberosoa CIG, Montoro P, Piacente S, Corona G, Deiana M, Assunta Dessi M, Pizaa C, Cabras P. Flavonoid characterization and antioxidant activity of hydroalcoholic extracts from Achillea ligustica All. J Pharmaceut Biomed Anal. 2009;50:440-448.
27. Bakkali F, Averbeck S, Averbeck, D, Idaomar M. Biological effects of essential oils: a review. Food Chem Toxicol. 2008;46:446-475.
28. Bezic N, Skocibucic M, Dunkic V, Radonic A. Composition and antimicrobial activity of Achillea clavennae L. essential oil. Phytother Res. 2003;17:1037-1040.
29. Toncer O, Basbag S, Karaman S, Diraz E, Basbag M. Chemical composition of the essential oils of some Achillea species growing wild in Turkey. Int J Agric Biol. 2010;12:527-530.
30. Candan F, Unlu M, Tepe B, Daferera D, Polissiou M, Sokmen A, Akpulat HA. Antioxidant and antimicrobial  activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae). Ethnopharmacol J. 2003;87:215-220.
31. Ghani A, Azizi M, Hassanzadeh-Khayyat M, Pahlavanpour AA. Essential Oil Composition of Achillea eriophora, A. nobilis, A. biebersteinii and A. wilhelmsii from Iran. Essent oils Bear Plants J. 2009;5:460-467.
32. Haziri AI,  Aliaga N, Ismaili M, Govori-Odai S, Leci O, Faiku F, Arapi V, Haziri I.  Secondary metabolites in essential oil of Achillea millefolium (L.) growing wild in east part of Kosova. Am J Biochem Biotechnol. 2010;6:32-34.
33. Jaimand K, Rezaee MB. Comparative study of the essential oils of three Achillea species from Iran. J Essent Oil Res. 2001;13:354-356.
34. Niazmand S, Esparham M. Cardiovascular effects of ‎aqueous ethanolic extract of Achillea wilhelmsii in rabbit. ‎Pharmacol online 2011;1:818-825.‎
35. Orav A, Kailas T, Ivask K. Composition of the essential oil from Achillea millefolium L. from Estonia. Essent Oil Res J. 2001;13:290-294.
36. Shafaghat A. Composition and antibacterial activity of the volatile oils from different parts of Achillea tenuifolia Lam. from Iran. Medicinal Plants J. 2009;8:93-98.
37. Tabanca N, Demirci B, Gürbüz I, Demirci F, Becnel JJ, Wedge DE, Başer HKC. Essential oil composition of five collections of Achillea biebersteinii from central Turkey and their antifungal and insecticidal activity. Nat Product Commun. 2011;6:701-706.
38. Pohanish RP. Toxic and Hazardous chemicals and carcinogens. Elsivier Inc. sixth edition. 2012; p. 380.
39. Mahmoudian M, Jalilpour H, Salehian P. Toxicity of Peganum harmala,Review and a Case Report. Iranian J Pharmacol Therapeut. 2002;1:1-4
40. Mockute D, Judzentiene A. Variability of the essential oils composition of Achillea millefolium Spp. millefolium growing wild in Lithuania. Biochem Syst Ecol. 2003;31:1033–1045.
41. Nickavar B, Abolhasani FA. Screening of antioxidant properties of seven Umbelliferae fruits from Iran. Pak J Pharm Sci. 2009;22:30‐35.
42. Dokhani S, Cottrell T, Khajeddin J, Mazza G. Analysis of aroma and phenolic components of selected Achillea species. Plant Foods Hum Nutr. 2005;60:55-62.
43. Hadaruga DI, Hadaruga NG. Antioxidant activity of Hepatoprotective Silymarin and Silybum marianum L. extract Chem. Bull. Politehnica Univ. (Timisoara). 2009;54:2.
44. Mazandarani M, Zarghami Moghadam P, Zolfaghari MR, Ghaemi E. Effect of ‎solvent type on TP and TF content and antioxidant activity in Onosma ‎dichroanthum  Boiss. in Golestan province, North of Iran. Med Plant Res ‎J. 2012;6:4481-4488.
‎45. Mazandarani M, Majidi Z, Zarghami Moghadam P, Abrodi M, Hemmati H, Fathi azad F. Essential oil composition , TP, TF, Anthocyanin and antioxidant activity in different parts of Artemisia annua L. in two localities, North of Iran. J Med Plants  By-prod. 2012;1:13-21.
46. Mukarram Shah SM, Khan FA, Hassan Shah SM, Chishti KA, Saifur Shah Pirzada SM, Asif Khan M, Farid A. Evaluation of phytochemicals and antimicrobial activity of white and blue Capitulum and whole plant of Silybum Marianum. World Appl Sci J. 2011;12:1139-1144.
47. Mazandarani M, Makari S, Bajian GR, Zarghami Moghadam P, Abrodi M. Evaluation of phytochemical and antioxidant activity in different parts of Heracleum gorganicum Rech.F. in Golestan province, North of Iran. Iranian J Plant Physiol. 2011;2:381-388.
48. Zarghami Moghaddam P, Mazandarani M, Zolfaghari MR, Ghaemi E. Anti bacterial and antioxidant activity in root extract of Onosma dichloroanthum Boiss. in North of Iran. Afr J Microbiol Res. 2012;6:1776-1781.
49. Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004;74:2157-2184.
50. Conforti F, Sosa S, Marrelli M, Menichini F, Statti GA, Uzunov D, Tubaro A, Fazly Bazzaz BS, Haririzadeh G, Imami SA, Rashed MH. Survey of Iranian plants for alkaloids, saponins and tannins (Khorasan province). Int J Pharmacol. 2008;35:17-30.
Journal of Medicinal plants and By-Products
Volume 2, Issue 1
April 2013
Pages 33-42

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