Influence of Plant Growth Regulators and Media on In vitro Propagation of Sorbus aucuparia L.


Aucuparia is an important slow growth forest tree with medicinal, industrial and ornamental uses which is applied for reforestation in high altitude of mountain lands. This species has been endangered at northern forests of Iran; therefore micropropagation of adult trees by bud culture may help to reforestation. The best sterilizing treatment was the buds washing with HgCl2% 0.1 solution for 7 minutes in Autumn. The highest of shoot regeneration were taken in DKW medium with BA (0.5 mg/l), IBA (0.1 mg/l) and TDZ (0.05 mg/l). Rooting of shoots was done in modified MCM medium supplemented with 1.5 mg/lof IBA in dark condition. The plantlets were acclimated in green house.


1. Chalupa V. In vitro propagation of mature trees of Sorbus aucuparia L and field performance of micropropagated trees. J Forest sci. 2002;48:529-535.

2. Morabito D, Mills D, Prat D and Dizengremel P. Response of clones of E. microtheca to NaCl in vitro. Tree Physiol. 1994;14:201-210.

3. Chalupa V. Micropropagation of mountain ash (Sorbus aucuparia L) and wild service tree (Sorbus torminalis L.). In: Bajaj YPS (eds.) Biotechnology in Agriculture and Forestry, Vol 18, Springer-Verlag publication, 1992, pp. 211-225.

4. Chalupa V. Clonal propagation of broud- leaved forest trees in vitro. Communication Institute for Cech. 1981;12: 255-271.

5. Chalupa V. In vitro propagation of willows, European mountain ash (Sorbus aucuparia L) and black locust. Biol Plantarum. 1983;25:305-315.

6. Chalupa V. Effect of benzyl amino purine and thydiazuron on in vitro shoot proliferation of Tilia cordata Mill., Sorbus aucuparia L. and Robinia pseudo acaciae L. Biol Plantarum. 1987;29:425-429.

7. Chalupa V. In vitro propagation of small – leaved linden, black locust and mountain ash (Sorbus aucuparia L.) and growth of tree cultivated in vitro. Lesnictvi. 1988; 34:705-720.

8. Custodio L, Carneiro M F, Romano A. Microsporogenesis and anther culture in carob tree (Ceratonia siliqua L.). Scientica Hort. 2005;104:65-77.

9. Souayah N, Khouja ML, Khaldi A, Rejeb MN, Bouzid S. Breeding improvement of Laurus nobilis L. by conventional and in vitro propagation techniques. J Herbs, Spices & Med Plants. 2002;9:101-105.

10. Driver JA, Kuniyuki H. In vitro propagation of Paradox walnut root stocks (J. hindsii* J. regia ). Hortscience. 1984; 19:507-509.

11. McCown B H and Sellmer J C. Media and physical environment. In: Bonga J M, Durzan D J (eds.) Cell and Tissue Culture in Forestry, VoL 1, Martinus Nijhoff Publishers, 1982, pp. 1-17.

12. Bonga J M and Aderkas P V. In vitro Culture of Trees. Kluwer Academic Publishers. 1992, p. 236.

13.Glock P, Delaporte K, Collins G and Sedgley M. Micropropagation of juvenile tissue of E. erythonema X Eucalyptus stricklandii cv. Urrbrae Gem. In vitro Cell Develop Biol Plant. 2006;42:139-143.

14. Onay A. In vivo and in vitro micrografting of pistachio (Pistacia vera L.). Turkish J Biol. 2003;27:95-100.

15. Assareh M H and Henney M J. Effects of CO2 - enriched and non- enriched environments on the growth rate of Eucalyptus microtheca. Research reports 1996/1997,UCD. 1998; pp.140-141.