Assessment of Genetic Diversity among Wild Populations of Achillea bieberstenii Afan. Using Agro-morphological and Germination Traits

Authors

Research Institute of Forests and Rangelands Gene Bank, Tehran, Iran

Abstract

There is substantial diversity in Achillea biebersteinii plants in Iran. It has considerable different biological activities including antibacterial, antifungal, antioxidant, insecticidal, herbicidal and wound healing. Research improvement for medicinal plants depends on the available genetic diversity. The assessment of genetic variability could increase the information for breeding programs. To evaluate possible diversity among different populations of A. biebersteinii, agro-morphological and germination traits of the populations were investigated. To evaluate agro-morphological traits, seeds of 22 populations were sown in the field. The experiment was laid out using Randomized Complete Block Design (RCBD) with three replications. A total of six agro-morphological traits including plant height, plant diameter, lateral shoot number, flower diameter, flower number and shoot yield, were collected. For evaluation of seed germination traits, seeds of 10 populations were sown in standard condition ((20±2 °C) and pre-cold treatment (7 days at 4 °C followed by standard condition). The seed germination traits were studied as hypocotyl length, radicle length, radicle to hypocotyl length ratio, seedling length, germination percentage,germination rate and vigor index. Based on the results the population 20187 (Golestan) had the highest value for all of agro-morphological traits. For germination traits the populations 10140 (Minoodasht) and 17310 (Shahrood) were the best performing populations. The result showed that pre cold treatment reduced radicle length in most cases but population 17310 (Shahrood) produced significantly longer radicle under pre-cold treatment. This population was recommended for dry cold regions.

Keywords

References

1. Asgari Rrad H, Pourmorad F, Hosseinimehr SJ, Saeidnia S, Ebrahimzadeh MA, Lotfi F. In vitro antioxidant analysis of Achillea tenuifolia. Afr J Biotechnol. 2010; 9:3536-3541.
2. Trumbeckaite S, Benetis R, Bumblauskiene L, Burdulis D, Janulis V, Toleikis A, Viškelis P, Jakštas V. Achillea millefolium L. S.L. herb extract: Antioxidant activity and effect on the rat heart mitochondrial functions. Food Chem. 2011;127:1540-1548
3. Rechinger, KH. Flora Iranica compositae VI-Anthemideae, Graz, Austria: Ackademiche Druck-U, 1986.
4. Rustaiyan A, Komeilizadeh H, Shariatpanahi M S, Jassbi A R, Masoudi S. Comparative study of essential oils of three Achillea species from Iran, J Essential Oil Res, 1998;10:207-209.
5. Sokmen A, Sokmen M, Daferera D, Polissiou M, Candan F, Unlu M, Akpulat H A. The in vitro antioxidant and antimicrobial activities of the essential oil and methanol extracts of Achillea biebersteinii Afan. (Asteraceae). Phytotherapy Res. 2004;18:451-456.
6. Baris O, Gulluce M, Sahin F, Ozer H, Kilic H, Ozkan H, Sokmen M, Ozbek T. Biological activities of the essential oil and methanol extract of Achillea biebersteiniii Afan. (Asteraceae). Turkish J Biol. 2006;30:65-73.
7. Kordali S, Cakir A, Akcin T A, Mete E, Akcin A, Aydin T, Kilic H. Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan. (Asteraceae). Industrial Crop Prod. 2009;29:562-570.
8. Bashi DS, Fazly Bazzaz BS, Sahebkar A, Karimkhani MM, Ahmadi A. Investigation of optimal extraction, antioxidant, and antimicrobial activities of Achillea biebersteinii and A. wilhelmsii. Pharm Biol. 2012; 50:1168-1176.
9. Zhang D, Armitage, AM, Affolter JM, Dirr MA. Environmental Control of Flowering and Growth of Achillea millefolium L. ‘Summer Pastels. Hortscience 1996;31:364-365.
10. Evenor D, Reuveni M Micro propagation of Achillea filipendulina cv. 'Parker'. Plant Cell Tissue Organ Cult. 2004;79:91-93
11. Ghany A, Azizy M, Tehranifar A. Evaluation of ornamental potential of 5 wild Achillea spp. Journal of Horticultural Science, Ferdosi University. 2009;23:25-31.
12. Khalil SK, Hilaire RS, Khan A, Rehman, Mexal JG. Growth and physiology of yarrow species Achillea millefolium cv. Cerise Queen and Achillea filipendulina cv. Parker Gold at optimum and limited moisture. Aust J Crop Sci. 2011;5:1698-1706.
13. Benedek B, Kopp B, Melizg MF. Achillea millefolium L. s.l. Is the anti-inflammatory activity mediated by protease inhibition? J Ethnopharmacol. 2007;113:312-317.
14. Mal TK, Lovett-Doust J. 2005 Phenotypic plasticity in vegetative and reproductive traits in an invasive weed, Lythrum salicaria (Lythraceae), in response to soil moisture. Am J Bot. 2007;92:819-825.
15. Ge S, Hong DY. Biosystematic studies on Adenophora potaninii Korsh. Complex (Campanulaceae) III. Genetic variation and taxonomic value of morphological characters. Acta Phytotaxonomica Sintica. 1995; 33:433-443.
16. Rahimmalek M, Sayed Tabatabaei B E, Arzani A, Etemadi N. Assessment of genetic diversity among and within Achillea species using Amplified Fragment Length Polymorphism (AFLP). Biochem Syst Ecol. 2009; 37:353-361.
17. Han J, Zhang W, Cao H, Chen S, Wang Y. Genetic diversity and biogeography of the traditional Chinese medicine, Gardenia jasminoides, based on AFLP markers Biochem. Syst Ecol. 2007;35:138-145
18. Rahimmalek M, Sayed Tabatabaei B E, Etemadi N, Hossein Golid S A, Arzania A, Zeinalie H. Essential oil variation among and within six Achillea species transferred from different ecological regions in Iran to the field conditions. Industrial Crops and Products. 2009;29: 348-355.
19. Gharibi S, Rahimmalek M, Mirlohi A, Majidi MM, Sayed Tabatabaei BE. Assessment of genetic diversity in Achillea millefolium subsp. millefolium and Achillea millefolium subsp. elbursensis using morphological and ISSR markers. J Med Plants Res. 2011;5:2413-2423
20. Mirahmadi SF, Hasandokht M.R, Sefidkon F, Hassani ME. A Comparative study on chemical constituents of essential oils from different populations of Achillea biebersteinii Afan. Growing wild in Khorasan using multivariate statistical analyses. Iranian J  Med Aromatic Plant. 2012;55:1-13.
21. Mirahmadi SF, Hasandokht MR, Hassani ME, Sefidkon F. Evaluation of genetic diversity among some wild populations of Achillea bieberstenii Afan. from Iran using morphological and agronomical traits. Int J Forest Soil and Erosion. 2012;2:8-17
22. Shariati M, Asemaneh T, Modares Hashemi S M. Effect of dormancy breaking treatments on Achillea millefolium. Pajouhesh-va-Sazandegi (in agronomy and horticulture) 2003;56-57:2-8.
23. Kaye T N, Liston A, Love RN, Luoma D L, Meinke R J, Wilson MV. Seed dormancy in high elevation plants: Implication for ecology and restoration. Corvallis Oregon. 1997, pp: 115-120.
24. Abdul- baki, A.A., Anderson J.D., Vigour determination in soybean seed by multiple criteria. Crop Sci. 1975;13:630-633.
25. Ward J H. Hierarchical grouping to optimize an objective function. J Am Stat Assoc. 1963;58:238-244.
26. Mozaffarian V. Compositae: Anthemideae and Echinopeae in Assadi et al. Flora of Iran no. 59. Tehran, 2008. (In Persian).
27. Pang G C, Jiang D M. Population genetic diversity and data analysis. Scientia Silvae Sinicae. 1995;31:543-550.
28. Baker H G. The evolution of weeds. Annual Review of Ecol Syst. 1974;5:1-24.
29. Sultan S E. Phenotypic plasticity for fitness components in Polygonum species of contrasting ecological breadth. Ecol. 2001;82:328-343.
30. Richards C L, Pennings S C, Donovan LA. Habitat range and phenotypic variation in salt marsh plants, Plant Ecol. 2005; 176:263-273.
31. Schlichting C D. Phenotypic plasticity in plants. Plant Species Biol. 2002;17:85-88.
32. Judzentiene, A, Mockute D. Essential oil composition of two yarrow taxonomic forms. Cent Europ J Biol (CEJB). 2012;5:346-352.
33. Ghani A, Tehranifar A, Shooshtarian S, Boghrat M. Comparative Study of Ornamental Potential of Six Achillea Species From Iran. South west J Hortic Biol Environ. 2011;2:139-155
34. Ghasemi Pirbalouti AN, Golparvar AR, Riyahi Dehkordy M, Navid A. The effect of different treatments on seeds dormancy and germination of five species of medicinal plants of Chahar Mahal & Bakhteyari province. Pajouhesh Sazandegi. 2007;74: 185-192.
35. Aminifar J, Mohsenabadi G, Bigloue H., Effect of zinc and potassium priming and salinity stress on germination and early seedling growth of corn (Zea mays L .var SC704). Int J Agric, 2013;3:580-583.
36. Krishnasamy V, Ramaswamy KR. Effect of N, P, and K on CSH-14 sorghum hybrid seed crop. II. Seed weight, germination and vigor. Madras Agri J. 1986;73: 42–5.
37. Zu J, Kang H, Tan H, Xu M. Effects of drought stresses induced by polyethylene glycol on germination of Pinus sylvestris var. mongolica seeds from natural and plantation forests on sandy land. J For Res. 2006;11:319-328.
38. Bosetti F, Montebelli C, L.C. Novembre AD, Chamma HP, Pinheiro JB. Genetic variation of germination cold tolerance in Japanese rice germplasm. Breeding Sci 2012; 62:209-215.
39. Sharifi, P. Evaluation on Sixty-eight Rice germplasms in Cold Tolerance at Germination Stage. Rice Science. 2010;17:77-81.
40. Hampton J.G., TeKrony D.M. Handbook of vigor test methods. Intl. Seed Testing Assn., Zurich, Switzerland 1995.
41. AOSA. Seed Vigor Testing Handbook. Contribution No. 32 to the Handbook on Seed Testing. Ithaca, NY. 2009 pp. 341.
42. Dalianis CD. Rate of radicle emergence as measure of seedling emergence and vigor in cotton (G. hirsutum L.). Seed Sci Technol 1982;10:35-45.
Journal of Medicinal plants and By-Products
Volume 2, Issue 2
September 2013
Pages 185-195

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