Synergistic Effect of Zataria multiflora Boiss. and Bunium persicum (Boiss.) B. Fedtsch. Essential Oils on Linseed Oil Oxidative Stability


Department of Food Science and Technology, Tarbiat Modares University, Tehran, Iran


We studied the antioxidant activities of the essential oils of Zataria multiflora Boiss. (ZEO) and Bunium persicum (Boiss.) B. Fedtsch (BEO) in mixed form on linseed oil using (DPPH), ­(­ABTS•+), H2O2 scavenging, and reducing power assays. After calculation of IC50 for ZEO and BEO separately, interactions of the essential oils were investigated at the form of mixture and the results were given in isobologram. The interactions between antioxidant effects of ZEO, BEO, TBHQ, and α-tocopherol; and isobologram results showed synergistic effect for DPPH except for BEO with TBHQ and α-tocopherol (1:1) and for ­ABTS•+ in ZEO with TBHQ (1:1). However, we could not find any synergistic effect for H2O2 scavenging and reducing power assays in any of the interactions. Statistical results showed that the best antioxidant levels of reductive oxidation were 600 ppm for ZEO and BEO, and 20 ppm for TBHQ in mixed form in linseed oil.


1. Akon CC. Min DB. Food Lipids: Chemistry, Nutrition and Biotechnology. 3th edn. CRC Press. USA.2008.
2. Dormana HJD, Peltoketo A, Hiltunen R,Tikkanen MJ. Characterization of the antioxidant properties of de-odourisedaqueous extracts from selected laminaceaeherbs, Food Chem.2003;83:255-262.
3. Harris R. Synergism in the essential oil. Inter J Aromatherapy.2002; 12:179-186.
4. May B, Kuntz D, Kieser M, Kohler S. Efficacy of a fixed peppermint/caraway oil combination in non-ulcer dyspepsia. Arzneim-Forsch.1996; 46:1149-1153.
5. Christoph F, Kaulfers PM, Stahl-Biskup E. In vitro evaluation of the antibacterial activity of b-triketones admixed to Melaleuca oils. Planta Med.2001;67:768-771.
6. Geda AK. Antibacterial activity of essential oils and their combinations. Fat. Sci. Technol. 1995;97:458-460.
7. Fyfe F, Armstrong F, Stewart J. Inhibition of Listeria monocytogenes and Salmonella enteriditis by combinations of plants oils and derivatives of benzoic acid: the development of synergistic antimicrobial combinations. Int. J Antimicrobial Agents.1998;9:195-199.
8. Tallarida R. Drug Synergism and Dose-Effect Analysis. Florida, CRC Press. USA. 247p. 2000.
9. Shahsavari N, Barzegar M, Sahari M A,Naghdibadi H.Antioxidant activity and chemical characterization of essential oil of B. persicum. Plant Foods Hum Nutr. 2008;63:183-188.
10. Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004;74:2157-2184.
11. Buyukbalci A, Nehir S. Determination of in vitro antidiabetic effects, antioxidant activities and phenol contents of some herbal teas. Plants Foods Human Nut.2008;63:27-33.
12. Hsu B, Couper IM, Ng K. Antioxidant activity of hot water extract from the fruit of the doum palm, HyphaeneThebaica.2006;98:317-328.
13. AOCS. In: D. Firestone, (Ed.), Official Methods and Recommend Practices of the American Oil Chemists’ Society (4thed.). Champaign: AOCS.1989.
14. Madsen HL, Sorensen B, Skibsted LH,Bertelsen G. The antioxidative activity of summer savory (Satureja hortensis L.) and rosemary (Rosmarinus oflcinalis L.) in dressing stored exposed to light or in darkness. Food Chem.1998;63:173-180.
15. Romano CS, Abadi K, Repetto V, Vojnov AA, Moreno S. Synergistic antioxidant and antibacterial activity of rosemary plus butylated derivatives. Food Chem.2009;115:456-461.
16. Svoboda KP, Hampson JB. Bioactivity of essential oils of selected aromatic plants: antibacterial, antioxidant, antiflammatory and other related pharmacological activities. Available on:
17. Rudnik E, Szczucinska A, Gwardiak H, Szulc A, Winiarska A. Comparative studies of oxidation stability of linseed oil. Thermochimica Acta.2001; 370:135-140.
18. Lampi AM, Kataja L, Eldin LK, Vieno P. Antioxidant activities of α- and γ-tocopherols in the oxidation of rapeseed oil triacylglycerols. JAOCS.1999;76:749-755.
19. Bandarra NM, Campos RM, Batista I, Nunes ML, Empis JM. Antioxidant synergy of α-tocopherol and phospholipids. JAOCS.1999;76:905-913.