Application of Embryo Rescue Technique in Juglans regia L. x J. nigra L. Hybridization

Author

Golestan Research Center of Agriculture and Natural Resources, P.O Box 4915677555 Gorgan, Iran

Abstract

Embryo culture were used to produce inter specific walnut hybrid plants between Juglans nigra L. and J. regia L. Developed ovaries were collected from J. rejia female flowers which were pollinated with J. nigra pollen grain by high dusting on a mature tree. In order to isolate embryos, exocarps were aseptically removed. Isolated embryos from ovules at different developmental stages were transferred to the different kind of growth-regulators hormone free media (MS, Half-MS, MS and Half-MS supplemented with 1 mg/l BA +0.1 mg/l NAA) for embryo development and germination. There was a significant difference between media for embryo germination α= 0.05 level. Highest percentage of embryo germination was observed in MS hormone free medium (13.7%).  Because of long period required for embryo development, isolation of embryo less than 45 days old embryos were impractical. There were a highly significant difference between age of embryos for embryo germination α= 0.01 level.   Embryo with more than 45 days old germinated and produced plants on all applied media. Highest germination rate were observed on cross-pollinated embryos which was isolated from 90 old ovaries.  Before being transferred to the potting soil and green-house, plantlets sub cultured in the same medium within jars. Thirteen successful acclimatized plantlets were transferred to the field.

Keywords

References

1. Jafari  MA, Modir-rahmati  A.  Production of Populus euphratica Oliv. × P. alba L. hybrid poplars through ovary and ovule cultures. Plant Genetic Newsletter. 2000:122:13-15.
2. Dandekar AM, Martin LA, McGranahan GH. Genetic transformation  and Foreign  gene expression in walnut tissue.  J. of American society for Horticultural Scince 1988:113;945-949.
3. Manning, WE. The classification within the Juglandaceae. Annals of Missouri Botanical Garden.  1978:65: 1058-1087.
4. McGranahan GH, Tulecke W, Arulsekar S. and Hansen JJ. Intergeneric hybridization in Juglandaceae: Petrocarya sp. x Juglans regia. J of American society for Horticultural Scince.  1986:111;627-630.
5. Clark J, Hemery G. and Savill  P. Early growth and form of common walnut (Juglans regia L.) in mixture with tree and shrub nurse species in southern England. Forestry. 2008:81:631-644.
6. Woeste  K. Heartwood production in a 35-year old black walnut progeny test. Can J Forest Res. 2002:32:177-181.
7. Jafari  MA, Modir-rahmati  A, Tavesoli A. Application of ovary and ovule culture in P. alba L x Populus euphratica OLIV hybridization. Silvae Genet. 1998:47:5 -6.
8. Ramming DW. The use of embryo culture in fruit breeding. HortScience  1990:25:393-398.
9. Kalagari M, Jafari MA. Tabari M, Hosseini : Intraspecific hybridization of Populus euphratica Oliv. Using in vitro technique. J of Sci Islamic Republic of Iran. 2004:15:109-122.
10. Li W, Wang R, Zhu  X. On the embryo development and ovule culture of interspecific hybrids between Populus simonii and P. pyramidalis Barkh. Acta Bot Sin. 1983:25:409-417.
11. Kouider M, Skirvin R M and Saladin K P. Culture immature embryo of Populus deltoides In Vitro. Can J For Res. 1984:14:965-958.
12. Li W, and Li J.  In vitro culture of hybrid ovules in Populus. Sci. Sliv. 21:339-346.
13. Raqiun C, and Trousard  L. In vitro ovary embryo culture as a tool for poplar hybridization Can J Bot, 1993:71:1271-1275
14. Payghamzadeh K. Somatic embryogenesis from immature cotyledons and meristemic culture of walnut (Juglans regia L.). The MSc thesis. College of agriculture, Dep of Plant breeding and Biotechnology, University of Agricultural and Natural Resources of Sari, Iran. 2008:pp 48-77.
15. Dumanoglu H. Dessiccation using saturated salt solutions and improvement germination rate of walnut (Juglans regia L.) somatic embryos. Turk J Agric For. 2000:24:491-498.
16. Cornu D, Jay-Allemand  C. Micropropagation of hybrid walnut trees (Juglans nigra × Juglans regia) through culture and multiplication of embryos. Ann. Sci. For 1988:46:113-116.
17. Kukharchyka N, Kastrickaya M. Embryo rescue techniques in  Prunus L. breeding. Journal of Fruit and Ornamental Plant Research Vol. 14 (Suppl. 1), 2006.
18. Pinto  ACQ, Rogers SMD, Byrne DH. Growth of immature peach embryos in response to media, ovule support method, and ovule perforation.Hort Science. 1994:29:1081-1083.
Journal of Medicinal plants and By-product
Volume 3, Issue 2
September 2014
Pages 211-214

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