Expression Pattern of pmt, erf1 and jap1 Genes in Nicotiana benthamiana and Atropa belladonna Plants under UV Radiation, Wounding and Methyl Jasmonate Treatments

Authors

Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.

Abstract

The Solanaceae plants produce a variety of interesting biologically active products including the steroid alkaloids solanidine, nicotine and tropane alkaloids. Putrescine N-methyltransferase (PMT) is an enzyme that catalyses s-adenosylmethionine-dependent methylation of putrescine in one of the primary steps of nicotine and tropane alkaloids biosynthesis pathway. Two tobacco members of the AP2/ERF-domain transcription factors family called NtORC1 and NtJAP1 were shown to up regulate the activity of the NtPMT promoter in   Nicotiana tabacum L. under environmental stresses. In this study,  semiquantitave RT-PCR analysis showed that expression patterns of PMT, ERF1 and JAP1 genes in shoots and roots of  Nicotiana benthamiana Domin and Atropa belladonna L. were examined under methyl jasmonate, UV radiation and wounding treatments. Plants were harvested half an hour after each treatment. The expression pattern of examined genes showed differences between plant tissues under different treatments.

Keywords

References

1. Staniek A, Bouwmeester HD. Fraser P, Kayser O, Martens S, Tissier A, Krol S-v-d, Wessjohann L, Warzecha H. Natural products–modifying metabolite pathways in plants. Biotech. J. 2013;8:1159-1171.
2. De Luca V, St Pierre B.The cell and developmental biology of alkaloid biosynthesis. Trends Plant Sci. 2000;5:168-173.
3. Sato F.Improved Production of Plant Isoquinoline Alkaloids by Metabolic Engineering. Adv. Bot. Res. 2013;68:163-181.
4. Ahmad S, Garg M, Tamboli E-T, Abdin M-Z, Ansari S-H.In vitro production of alkaloids: Factors, approaches, challenges and prospects. Pharmacog. Rev. 2013;7:27.
5. Veeresham C. Natural products derived from plants as a source of drugs. J. Adv. Pharmacol. Rech. Resh. 2012; 3:200-201.
6. Glenn W-S, Runguphan W, O’Connor S-E. Recent progress in the metabolic engineering of alkaloids in plant systems. Curr. Opin. Biotech. 2013;24:354-365.
7. Junker A, Fischer J, Sichhart Y, Brandt W,Dräger B. Evolution of the key alkaloid enzyme putrescine N-methyltransferase from spermidine synthase. Front. Plant Sci. 2013.4.
8. Moses T, Pollier J, Thevelein J-M, Goossens A. Bioengineering of plant (tri) terpenoids: from metabolic engineering of plants to synthetic biology in vivo and in vitro. New Phytologist. 2013; 200: 27-43.
9. T. Todd A, Liu E, L. Polvi S, T. Pammett R, E. Page J.A functional genomics screen identifies diverse transcription factors that regulate alkaloid biosynthesis in Nicotiana benthamiana. Plant J. 2010;62:589-600.
10. Miralpeix B, Rischer H, T. Häkkinen S, Ritala A, Seppänen-Laakso T, Oksman-Caldentey K-M, Capell T, Christou1 P.Metabolic engineering of plant secondary products: which way forward?. Curr. Pharmaceut. Des. 2013;19:5622-5639.
11. Sachan N, L.Falcone D. Wound-induced gene expression of putrescine N-methyltransferase in leaves of Nicotiana tabacum. Phytochemistry. 2002;61:797-805.
12. Shoji T, Kajikawa M, Hashimoto T.Clustered transcription factor genes regulate nicotine biosynthesis in tobacco. The Plant Cell Online. 2010;22:3390-3409.
13.De -Boer K, Tilleman S, Pauwels L, Bossche V-R, De Sutter V, Vanderhaeghen R, Hilson P, D. Hamill J, Goossens A. APETALA2/ETHYLENE RESPONSE FACTOR and basic helix–loop–helix tobacco transcription factors cooperatively mediate jasmonate‐elicited nicotine biosynthesis. Plant J.  2011;66:1053-1065.
14. Shoji T, Hashimoto T. Tobacco MYC2 regulates jasmonate-inducible nicotine biosynthesis genes directly and by way of the NIC2-locus ERF genes. Plant Cell  Physiol. 2011;52:1117-1130.
15. Mizusaki S, Tanabe Y, Noguchi M, Tamaki E. Changes in the activities of ornithine decarboxylase, putrescine N-methyltransferase and N-methylputrescine oxidase in tobacco roots in relation to nicotine biosynthesis. Plant Cell Physiol. 1973;14:103-110.
16. Riechers D-E, Timko M-P. Structure and expression of the gene family encoding putrescine N-methyltransferase in Nicotiana tabacum: new clues to the evolutionary origin of cultivated tobacco. Plant Mol. Bio. 1999;41:387-401.
17. Ramani S, Chelliah J. UV-B-induced signaling events leading to enhanced-production of catharanthine in Catharanthus roseus cell suspension cultures. BMC Plant Biol. 2007;7: 61.
18. Zhao J, Davis L-C, Verpoorte R. Elicitor signal transduction leading to production of plant secondary metabolites. Biotech Adv. 2005;23:283-333.
19. AK. Jansena M, Hectors KM. O’Brien N, Guisez Y, Potters G. Plant stress and human health: Do human consumers benefit from UV-B acclimated crops?.  Plant Sci. 2008;175:449-458.
20. Raina S-K, Wankhede D-P, Jaggi M, Singh P, Jalmi S-K, Raghuram B, Sheikh A-H, Sinha A-K. CrMPK3, a mitogen activated protein kinase from Catharanthus roseus and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids. BMC Plant Biol. 2012;12:134.
21. Xu B, Timko M.Methyl jasmonate induced expression of the tobacco putrescine N-methyltransferase genes requires both G-box and GCC-motif elements. Plant  Mol. Biol. 2004;55:743-761.
22. Winz R-A, Baldwin I-T. Molecular Interactions between the Specialist Herbivore Manduca sexta (Lepidoptera, Sphingidae) and Its Natural Host Nicotiana attenuata. IV. Insect-Induced Ethylene Reduces Jasmonate-Induced Nicotine Accumulation by Regulating Putrescine N-Methyltransferase Transcripts. Plant Physiol. 2001;125:2189-2202.
Journal of Medicinal plants and By-Products
Volume 4, Issue 1
April 2015
Pages 1-8

Files

Share

How to cite

Statistics