Comparative Analysis of Intra-and Inter Populational Heterogeneity of the Essential Oils in White Savory Plants

Authors

1 Department of Horticulture, Gorgan University of Agricultural Sciences and Natural Resources, Iran

2 Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, 38156-8-8349, Iran.

3 Medicinal Plants and Drug Research Institute, Shahid Beheshti University, G.C., Evin, 1483963113 Tehran, Iran

Abstract

White savory(Satureja mutica Fisch & C.A.Mey.) is one of the most widely used medicinal plants in food processing, pharmaceutical and cosmetic industry due to the strongly scented and presence of phenolic compounds such as carvacrol and thymol. This experiment was carried out to evaluate the levels of inter and intra-populations variability of essential oil compositions of S. mutica grown in north of Iran. The essential oil was extracted by hydro-distillation method and analyzed using GC-FID and GC-MS apparatus. The results showed a high level of variation among individual plants of the studied populations based upon their essential oil production. The essential oil content ranged from 0.5 to 4.2%. thymol (6.5-74.6%), carvacrol (0.9-70.4%), borneol (0.1-38.0%), p-cymene (0.30-14.2%), and γ-terpinene (0.1-9.9%) were recognized as the major components of the all tested individual plants. Therefore, the variability identified here, might be considered as characterizing the large gene pool for breeding programs to comply the requirements of pharmaceutical and food industries.

Keywords


1. Jamzad Z. Thymus and Satureja species of Iran, Research Institute of Forests and Rangelands press. Tehran, 2009; pp. 121.
2. Zargari A. Medicinal Plants. Tehran University Publications, Tehran, 1990; vol. 4. pp. 42-45.
3. Sefidkon F, Jamzad Z and Mirza M. Chemical variation in the essential oil of Satureja sahendica from Iran.Food Chem. 2004; 88:325-328.
4. Sefidkon F and Jamzad Z. Chemical composition of the essential oil of three Iranian Satureja species (S. mutica, S. macrantha and S. intermedia). Food Chem. 2005;91:1-4.
5. Gohari AR, Hadjiakhoondi A, Shafiee A, Ebrahimi ES and Mozaffarian VA. Chemical composition of the essential oils of Satureja atropatana and Satureja mutica growing wild in Iran. J. Essent. Oil Res. 2005; 17:17–18.
6. Gulluce M, Sokmen M, Sahin F, Sokmen A, Adiguzel A and Ozer H. Biological activities of the essential oil and methanolic extract of Micromeria fruticosa (L) Druce ssp serpyllifolia (Bieb) PH Davis plants from the eastern Anatolia region of Turkey. J. Sci. Food Agric. 2004;84:735–741.
7. Hadian J, Nejad-Ebrahimi S and Salehi P. Variability of morphological and phytochemical characteristics among Satureja hortensis L. accessions of Iran. Ind. Crop Prod. 2010;32:62-69.
8. British Pharmacopeia. Her Majesty Stationery Office, HMSO, 1993; London, UK.
9. Adams RP. Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy, 4th ed. Allured Publishing. 2007; Carol Stream, IL.
10. Hadian J, Mirjalili MH, Kanani MR, Salehnia A, Ganjipoor P. Phytochemical and morphological characterization of Satureja khuzistanica Jamzad Populations from Iran. Chem. Biodivers. 2011;8:902-915.
11. Karousou R,  Koureas DN and Kokkini S. Essential oil composition is related to the natural habitats: Coridothymus capitatus and Satureja thymbra in NATURA 2000 sites of Crete. Phytochem. 2005;66:2668–2673.
12. Lee LS, Brooks LO, Homer LE, Rossetto M, Henry RJ, and Baverstock PR. Geographic variation in the essential oils and morphology of natural populations of Melaleuca alternifolia (Myrtaceae). Biochem. Syst. Ecol. 2002;30:343-360.
13. Agostini G, Agostini F, Atti-Serafini L and Echeverrigaray S. Essential oil variability within and among populations of Cunila incisa Benth. Biochem. Syst. Ecol 2006;34:802-808.
14. Munoz-Bertomeu J, Arrillaga I and Segura J. Essential oil variation within and among natural populations of Lavandula latifolia and its relation to their ecological areas. Biochem. Syst. Ecol. 2007;35:479-488.
15. Dardioti A, Karousou R, Lanaras T and Kokkini, S. Diversity of Satureja pilosa subsp. origanita essential oils: A new Oregano from East Mediterranean. Biochem. Syst. Ecol. 2012;40:178-183.
16. Herraiz-Penalver D, Angeles Cases M, Varela F, Navarrete P, Sanchez-Vioque R and Usano-Alemany J. Chemical characterization of Lavandula latifolia Medik. essential oil from Spanish wild populations. Biochem. Syst. Ecol. 2013;46:59-68.
17. Valero M and Giner MJ. Effects of antimicrobial components of essential oils on growth of Bacillus cereus INRA L2104 in and the sensory qualities of carrot broth. Int. J. Food Microbiol. 2006;106:90-94.
18. Delgado B, Fernandez PS, Palop A, Periago and PM. Effect of thymol and cymene to establish safe conditions related to Bacillus cereus vegetative cells through the use of frequency distributions. Food Microbiol. 2004;21:327-334.
20. Yanishlieva N, Marinova E, Gordon M and Raneva V. Antioxidant activity and mechanism of action of thymol and carvacrol in two lipid systems. Food Chem. 1999;64:59-66.