Somatic Embryogenesis and Plant Regeneration in Evening Primrose (Oenothera biennis L.)

Authors

Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

Abstract

Callus induction responses and regeneration through callus-mediated SE were studied from evening primrose (Oenothera biennis L.) as an important medicinal plant of Onagraceae mainly known for its gamma-linoleic acid (GLA) content. The effects of cytokinins, 6-Benzylaminopurine (BAP) and N-(2-furfurylamino)1-H-purine-6-amine [Kinetin (Kin)] and their concentrations (0, 0.5, 1.0 and 1.5 mg/l) in combinations with 2,4-dichlorophenoxyacetic acid (2, 4-D) concentrations (0.25, 0.75 mg/l) on callus induction and SE for three explant types (petiole, leaf and epical bud) of "Shiraz" variety were separately evaluated. In all types of explants, the highest callus fresh weights belonged to 1 mg/l BAP or Kin in combinations with 2, 4-D (either 0.25 mg/l or 0.75 mg/l). The maximum fresh weight of callus was obtained from leaf explants plated on culture medium containing of 0.25 mg/l 2, 4-D and 1 mg/l Kin. The maximum The maximum number of embryos was achieved from leaf explants related to 0.75 mg/l 2, 4-D and 1 mg/l Kin. plantlets were successfully raised from in vitro developed embryos. Efficient plant regeneration via SE may provide a reliable system for studying the molecular mechanism of SE and a route for the genetic transformation of evening primrose.

Keywords

References

1. Greiner S, Köhl K. Growing evening primrose (Oenothera). Front Plant Sci. 2014;5:38.
2. Dietrich W, Wagner WL, Raven PH. Systematics of Oenothera section Oenothera subsection Oenothera (Onagraceae). Syst Bot Monogr. 1997;50:1-234.
3. Kerscher MJ, Korting HC. Treatment of atopic eczema with evening primrose oil: rationale and clinical results. Clin Investig. 1992;70:167-171.
4. Deng Y, Hua HM, Li J, Lapinskas PP. Studies on cultivation and use of evening primrose (Oenothera spp.) in China. Econ Bot. 2001;55:83-92.
 5. Ghasemnezhad A, Honermeier B. Effects of Nitrogen and Pre-Harvest Desiccation on Seed Yield and Oil Quality of Evening Primrose (Oenothera biennis L.). J Med Plants By-prod. 2012;1:61-65.
6. Quiroz-Figuerosa FR, Rojas-Herrera R, Galaz-Avalos RM, Loyola-Vargas VM. Embryo production through somatic embryogenesis can be used to study cell differentiation in plants. Plant cell Tiss Org cult. 2006;86: 285-301.
7. Redenbaugh K, Fujii JAA, Slade D. Hydrated coating for synthetic seeds. In: Redenbaugh K. (Ed.) Synseeds: Applications of synthetic seeds to crop improvement, CRC Press, Boca Raton, FL, 1993, pp. 35‐46.
 8. Mamiya K, Sakamoto Y. A method to produce encapsulatable units for synthetic seeds in Asparagus officinalis. Plant Cell Tiss Org Cult. 2001;64:27‐32.
 9. Vicient CM, Martinez FX. The potential uses of somatic embryogenesis in Agroforestry are not limited to synthetic seed technology. Rev Bras Fisiol Veg. 1998;10:1-12.
10. Kamle M, Bajpai A, Chandra R, Kalim S, Kumar R. Somatic embryogenesis for crop improvement. GERF Bull Biosci. 2011;2:54-59.
11. EL-Sawy A, Gomaa A, Abd-El-Zaher MH, Reda A, Danial N. Production of Somatic Embryogenesis via in vitro Culture of Stigma and Style for Elimination of Citrus Psorosis Virus (CpsV) from Some Citrus Genotypes. J Hort Sci & ornamen plants. 2013;5:110-117.
12. Ebrahimi MA, Payan A. Induction of Callus and Somatic Embryogenesis from Cotyledon Explants of Fagonia indica Burm. J Med Plants By-prod. 2013;2:209-214.
13. Ghasemnezhad A, Mousavizadeh SJ, Mashayekhi A. A study on evening-primrose (Oenothera biennis L.) callus  regeneration and somatic embryogenesis. Iran J Biotechnol. 2011;9(1):31-36.
14. Faramarzi Hafez R, Shahabzadeh Z, Heidari B, Ghadimzadeh M. Investigation of the Efficiency of Direct and Indirect Regeneration in Evening Primrose (Oenothera biennis). J Crop Sci Biotechnol. 2013;16:291-296.
15. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473-476.
16. Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968;50:151-158.
17. Abdi Gh, Khosh-Khui M. Shoot regeneration via direct organogenesis from leaf segments of Valerian (Valeriana officinalis L.). Int J Agric Res. 2007;2:877-882.
18. Ebrahimzadeh M, Shaker H, Bernard F, khavarinezhad RA. Effect of hormons and explant in callus induction and plant regeneration in tissue culture of anthurium andreanum var. Tropical. Pajouhesh-Va-Sazandegi. 2007;19:169-176. (In Persian)
19. Hoori F, Ehsanpour AA, Mostajeran A. Comparison of somatic embryogenesis in Medicago sativa and Medicago truncatula. Pak J Biol Sci. 2007;10:481-485.
20. Visser C, Qureshi JA, Gill R, Saxena PK. Morphoregulatory role of thidiazuron, substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures. Plant Physiol. 1992;99:1704-1707.
21. Ramin AA, Kashi AK, Etemadi N. The effect of hormone combination and explants on garlic (Allium sativum L.) callus induction in vitro. The Scientific Journal of Agriculture. 2002;25:1-11. (In Persian)
22. Anzidei M, Bennici A, Schiff S, Tani C, Mori B.Organogenesis and somatic embryogenesis in Foeniculum vulgare: histological observations of developing embryogenic callus. Plant Cell Tiss Org Cult.2000;61:69-79.
23. Conde P, Sousa A, Costa A, Santos C. A protocol for Ulmus minor Mill. Micropropagation and acclimatization. Plant Cell Tiss Org Cult. 2008;92:113-119.
24. Feyissa T, Welander M, Negash L. In vitro regeneration of Hagenia abyssinica (Bruce) J.F. Gmel. (Rosaceae) from leaf explants. Plant Cell Rep. 2005;24:392-400.
25. Landi L, Mezzetti B. TDZ, auxin and genotype effects on leaf organogenesis in Fragaria. Plant Cell Rep. 2006;25:281-288.
26. Ozaslan M, Can C, Aytekin T. Effect of explant source on in vitro propagation of Paulownia tomentosa Steud. Biotechnol Biotec Eq. 2005;19:20-26.
27. Reichert NA, Young MM, Woods AL. Adventitious organogenic regeneration from soybean genotypes representing nine maturity groups. Plant Cell Tiss Org Cult. 2003;75:273-277.
28. Rodríguez A, Cervera M, Peris JE, Pena L. The same treatment for transgenic shoot regeneration elicits the opposite effect in mature explants from two closely related sweet orange (Citrus sinensis (L.) Osb.) genotypes. Plant Cell Tiss Org Cult. 2008;93:97-106.
Journal of Medicinal plants and By-Products
Volume 5, Issue 1
April 2016
Pages 1-6

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