Genetic Relationships among Three Yarrow Species Based on Phenotypic Traits and Peroxidase Profiling

Authors

1 Natural Resources Gene Bank, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization, Tehran, Iran

2 Department of Botany, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization, Tehran, Iran

Abstract

Fifteen yarrow populations from different species Achillea millefolium L., A. biebersteinii L. and A. nobilis, from different geographical areas of Iran were studied using 24 morphological traits and peroxidase profiles. Comparison of mean values of different phenotypic traits show A. millefolium and A. biebersteinii L. had higher plant height and crown diameter; however, A. nobilis had higher dry matter yield and 1000-grain weight. Clustering pattern, made on the basis of different phenotypic traits, grouped the Achillea populations differently and gave no clear indication of origin or species. The results of peroxidase profiles revealed that the genetic diversity of A. nobilis samples was considerably higher than in A. millefolium and A. biebersteinii. Principal coordinate analysis revealed a clear separation between the different Achillea species. The results demonstrated that the study of genetic diversity and relationships among Achillea species using phenotypic traits and peroxidase profiles provides important information for the collection, conservation and the planning of future breeding programs.

Keywords


1. Rechinger KH. Flora Iranica, No. 158, Akademische Druke-U, Verlagsanstalt, Wien, 1963, pp. 49-71.
2. Mozaffarian V. A dictionary of Iranian plant names. Farhang Moaser Publishers, Tehran, Iran, 2007, pp. 11-22.
3. Bartram T. Encyclopedia of Herbal Medicine. Dorset: Grace Publishers. 1995, p.181.
4. Salehi Shanjani P. Evaluation, identification and propagation of yarrow Achillea spp. seeds in natural resources gene bank of Iran. Final technical report no. 43905. Research Institute of Forests and Rangelands, Iran, 2013, 250 p.
5. Ghafoor A, Ahmad Z, Qureshi AS, Bashir M. Genetic relationship in Vigna mungo L. Hepper and V. radiate L. R. Wilczek based on morphological traits and SDS-PAGE. Euphytica.2002;123:367-378.
6. Benedek B, Kopp B, Melizg MF. Achillea millefolium L. - Is the anti-inflammatory activity mediated by protease inhibition? J Ethnopharma. 2007;113:312-317.
7. Farajpour M, Ebrahimi M, Amiri R, Noori SAS, Golzari R. Investigation of variations of the essential oil content and morphological values in yarrow Achillea santolina. from Iran. J medicin Plant. Res. 2011;5:4393-4395.
8. Gurevitch J. Sources of variation in leaf shape among two populations of Achillea lanulosa. Genetics. 1992;130:385-394.
9. Miller MP. AMOVA-PREP, a program for the preparation of AMOVA input files for use with WINAMOVA. Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ. 1997.
10. Morsy AA. Molecular variations of Achillea fragrantissima Forssk.. SCH. BIP. growing in five areas of South Sinai. Int J Agri Biol. 2007;9:11-17.
11. Nadim MM, Malik AA, Ahmad J, Bakshi SK. The Essential Oil Composition of Achillea millefolium L. Cultivated under Tropical Condition in India. World J Agri Sci. 2011;7:561-565.
12. Rawashdeh IM. Genetic diversity analysis of <