High-Frequency in Vitro Direct Shoot Regeneration from Nodal Explants of Hyssop Plant (Hyssopus officinalis L.)

Authors

1 Horticulture Department, Agriculture Faculty, Urmia University, Urmia, Iran

2 Plant Breeding and Biotechnology Department, Agriculture Faculty, Urmia University, Urmia, Iran

3 Field Crops Department, Agriculture Faculty, Urmia University, Urmia, Iran

Abstract

Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitro culture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of TDZ and BAP in combination with 1 µmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01). In BAP treatments, the maximum shoot-buds induction (9 shoot-buds per explant) and shoot regeneration percentage (96.66%) were observed on MS medium fortified with 2.2 and 4.4 µmol BAP in combination with 1 µmol of IAA. In TDZ treatments, the highest regeneration percentage was achieved in MS medium supplemented with TDZ (2.2 µmol) and IAA (1 µmol), and the maximum shoot-buds induction (19.83 shoot-buds per explant) was observed in medium containing 4.4 µmol of TDZ in combination with 1 µmol of IAA. The highest root production frequency (89.5%) was achieved in medium contained 9.84 µmol of IBA. Rooted plants were acclimatized successfully in greenhouse conditions with 100% survival. The protocol described here could be applicable for mass in vitro production of the valuable medicinal plant Hyssopus officinalis L. for its genetic resource conservation as well as pharmaceutical purpose.

Keywords


1. Sokovia DM. Antifungal activities of essential oils of selected aromatic and medical plants in vitro and in vivo. Ph.D. Thesis, Faculty of Biology, University of Belgrade, Yugoslavia. 2001;650pp.

2. Cantrell Ch, Franzblau S, Fischer N. Anti-mycobacterial plant terpenoids. Planta Medica, 2001;67:685–694.

3. Omidbeigi R. Production and processing of medicinal plants. behnashr publisher. 2009;1: 350pp.

4. Chaturdedi HC, Jain M, Kidwai NR. Cloning of medicinal plants through tissue culture- A review. Indian J Exp Biol. 2007;45:937-948.

5. Tripathi L, Tripathi J. Role of biotechnology in medicinal plant. Trop J Pharm Res. 2003;2:243-253.

6. Misra P, Chaturvedi H. Micropropagation of Rosmarinus Officinalis L. Plant Cell Tiss Organ Cult. 1984;3:163-168.

7. Gulatia A, Bharel S, Abdin MZ, Jain S, Srivastava P. In vitro micropropagation and flowering in Artemisia annua. J Plant Biol Biotechnol. 1996;5:31-37.

8. Nanova Zh, Salavova D, Ivanova I. Microclonal Propagation of hyssop (Hyssopus officinalis L.). Bulg J Agri Sci. 2007;13:213-219.

9. Guo G, Gao M, Chun-Zhao L. In vitro propagation of an endangered medicinal plant. Handbook and Directory of Commerical Laboratories. Eversley, Basingstoke, Hants, England. 2006;700 pp.

10. Shan-shan H, Chun-zhao L, Praveen S. Plant regeneration of an endangered medicinal plant Hydrastis canadensis L. Sci Hortic-Amsterdam. 2007;113: 82-86.

11. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 1962;15:473-476.

12. Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968;50:151-158.

13. Cline MG. Exogenous auxin effects on lateral bud outgrowth in decapitated shoots. Ann Bot. 1996;78:255-266.

14. Dai Y, Wang H, Li, B, Huang J, Liu, X, Zhou Y, Mou Z, Li J. Increased expression of mapkinase kinase causes deficiency in polar auxin transport and leads to plant architectural abnormality in Arabidopsis. Plant Cell. 2006;18:308-320.

15. Punyarani K, Sharma GJ. Micropropagation of Costus speciosus (Koen.) using nodal segment culture. J Nat Sci Bio Med. 2010;2:58-62.

16. Sahoo Y, Chand PK. In vitro multiplication of medical herb Tridax procumbens L. (Mexican daisy, coat button) influence of explanting season, growth regulator, synergy, culture passage and passing substrate. Phytomorphology. 1998;48:195-205.

17. Smolenskaya SA, Ibragimova SS. Restorative Morphogenesis in vitro in the Annual Medics in the Presence of Benzylaminopurine. Russ J Dev Biol. 2002;33:349-354.

18. Siddique I, Anis M (2008) an improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L. Acta Physiol Plant. 2008;30:493-499.

19. Gopi C, Nataraja Sekhar, Y, Ponmurugan P. In vitro multiplication of Ocimum gratissimum L. through direct regeneration. Afr J Biotechnol. 2005;5:723-726.

20. Chen JG. Dual auxin signalling pathways control cell elongation and division. J Plant Growth Regul. 2001;20:255-264.

21. sivanesan I, Jeong BR. Direct shoot regeneration from nodal explabts of sida cordifolia Lnn. in vitro. Cell Dev Biol Plant. 2007;43:436-441.

22. Sunil B, Abdullah JO, Sreeramanan S, Karuthan C. Shoots Induction from Hibiscus Rosa-sinensis Nodal Explant Using N6-benzylaminopurine (BAP). Res J Agr Biol Sci. 2009;5:403-410.

23. Amutha R, Jawahar M, Ravi Paul S. Plant regeneration and in vitro flowering from shoot tip of Basilicum polystachyon L. An important medicinal plant. J Agric Sci Technol. 2008;4:117-123.

24. Muhammad Z, Riaz-ur R, Muhammad F (2007) Hormonal regulation for callogenesis and organgenesis of Artemisia absinthium L. Afr J Biotechnol. 2007;6:1874-1878.

25. Gabriela F, Luciani A, Mary C, Curvetto N. Effect of explant and growth regulators in garlic callus formation and plant regeneration. Plant Cell Tiss Organ Cult. 2006;87:139-143.

26. Roberson D, Cristiane L, Francine L, Henrique K, Marguerite Q. Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis. Agr Sci. 2005;62:406-412.

27. Shalaka DK, Sandhya P. Micropropagation and organogenesis in Adhatoda vasica for the estimation of vasine. Pharmacognosy Mag. 2009;5:539–363.

28. Kim OT, Kim MY, Hong MH, Ahn JC, Huang B. Stimulation of asiticoside accumulation in the whole plant cultures of Centella asiatica Urban by elicitors. Plant Cell Rep. 2004;23:339-344.

29. Grzegorczyk I, Wysokinska H. Liquid shoot culture of Salviaofficinalis L. for micropropagation and production of antioxidant compounds. Acta Soc Bot Pol. 2008;73:99-104.

30. Kornfeld A, Kaufman PB, Lu CR, Gibson DM, Bolling SF, Warber SL, Chang SC, Kirakosyan A. The production of hypericins in two selected Hypericum perforatum shoot cultures is related to differences in black gland culture. Plant Physiol Bioch. 2007;45:24-32.

31. Gu X, Zhang J. an efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujube Mill.) using leaf explants. Plant Cell Rep. 2007;23:775-779.

32. Ipecki Z, Gozukirmi N. Indirect somatic embryogenesis and plant regeneration from leaf and internode explants of Paulownia elongate. Plant Cell Tiss Organ Cult. 2004;79:341-345.

33. Edwin R, Paul D. Plant propagation by Tissue Culture. Handbook and Directory of Commerical Laboratories. First published. Exegetics Ltd. England. 1984;700 pp.

34. Oluk E, Cakir A. Micropropagation of Origanum sipyleum L. an endemic medicinal herb of Turkey. Afr J Biotechnol. 2009;8:5769-5772.