Effects of Irrigation Interval and Nitrogen Amount on Different Clary Sage (Salvia sclarea L.) Characters in Karaj

Authors

Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

Abstract

Salvia sclarea L, is an important medicinal plant that its oil has been evaluated for antioxidant, antibacterial, antifungal, anti-inflammatory, antimalarial, anticholinesterase and antiviral. Yield of plants are influenced by environmental and agricultural management factors such as drought stress and nutrition. Plant nutrition is one of the most important factors that positively influence plant production. Nitrogen, an essential plant nutrient is the most recognized in plants for its presence in the structure of the protein molecule. This experiment was conducted in 2011 in Alborz Research Station, Research Institute of Forests and Rangelands, Karaj, Iran. In order to evaluate the effect of drought stress and nitrogen on traits of Clary Sage, The experiment was conducted in split plot based on a randomized complete block design with three replications. The main factor was irrigation period (every 3, 6 and 9 days) and the sub factor was nitrogen application (0,100 and 200 and 400 kg/ha). Results indicated that irrigation interval significantly affected all the measured traits except for petiole yield, chlorophyll b and RWC. Nitrogen significantly affected all the measured traits except for leaf yield, chlorophyll b and RWC. The interaction of irrigation×nitrogen had also a significant effect on all the measured traits. Shoot yield was the highest in the interaction of 3 days×200 kg/ha (3680kg/ha). Oil percentage was the highest in the interaction of 9 days×0 kg/ha (0.05%). This experiment briefly indicated that clary sage can be cultivated in areas with low water supply, and it can produce the highest essential oil yield about 1400 kg/ ha when irrigated every 9 days without fertilizer.

Keywords


1. Pobedimova EG. Salvia L. Flora of USSR. 2000;21: 244-363.
2. Li XW, Hedge IC, Lamiaceae (Labiatae), Flora of China.1994;17:195-222.
3. Werker E, Ravid U, Putievsky E. Glandular hairs and their secretions in the vegetative and reproductive organs of Salvia sclarea and S. dominica. Israel J Bot. 1985;34:239-252.
4. Annex to the Official Journal of the European Communities 1990;C 146 A.
5. Setzer WN. Essential oils and anxiolytic aromatherapy, Nat. Prod. Commun. 2009;4:1305-1316.
6. Fraternale D, Giamperi L, Bucchini A, Ricchi D, Epifano F, Genovese S, Curini M. Composition and antifungal activity of essential oil of Salvia sclarea from Italy. Chem Nat Comp. 2005;41:604-606.
7. Kintzios SE. Sage – The Genus Salvia. Harwood, Amsterdam, Netherlands. 2000.
8. Ögütçü H, Sökmen A, Sökmen M, Polissiou M, Serkedjieva J, Daferera D, Sahın F, Baris Ö, Güllüce dM,. Bioactivities of the various extracts and essential oils of Salvia limbata C.A.Mey. and Salvia sclarea L. Turk J Biol. 2008;32:181-192.
9. Kuzma Ł, Kalemba D, Róalski M, Róalska F, Wieckowska-Szakiel M, Krajewska U, Wysokinska H. Chemical composition and biological activities of essential oil from Salvia sclarea plants regenerated in vitro, Molecules. 2009;14:1438-1447.
10. Jirovetz L, Buchbauer G, Denkova Z, Slavchev A, Stoyanova A, Schmidt E. Chemical composition, antimicrobial activities and odor descriptions of various Salvia sp. and Thuja sp. essential oils. Nutrition. 2006;90:152-159.
11. Pitarokili D, Couladis M, Petsikos-Panayotarou N, Tzakou O. Composition and antifungal activity on soil-borne pathogens of the essential oil of Salvia sclarea from Greece, J. Agric. Food Chem. 50, 6688-6691. Sharopov & Setzer. Res Nat Prod. (200X) . 2002;6:175-7979
 12. Džamic A, Sokovic M, Ristic M, Grujic-Jovanovic S, Vukojevic J, Marin PD. Chemical composition and antifungal activity of Salvia sclarea (Lamiaceae) essential oil. Arch Biol Sci. 2008;60:233- 237.
13. Moretti MDL, Peana AT, Satta M. A study on anti-inflammatory and peripheral analgesic action of Salvia sclearea oil and its main components, J Essent Oil Res. 1997;9:199-204.
14. Özek T, Tabanca N, Demirci F, Wedge DE, Baser KHC. Enantiomeric distribution of some linalool containing essential oils and their biological activities. Res Nat Prod. 2010;4:180-192.
15. Orhan I, Kartal M, Kan Y, Sener B. Activity of essential oils and individual components against acetyl- and butyrylcholinesterase, Z. Naturforsch. 2008;63c:547-553.
16. Dikova B. Establishment of some viruses – polyphagues on economically important essential oil– bearing and medicinal plants in Bulgaria. Biotech. 2009;23:80-85.
17. Çınar ÖG, Kirmizibekmez H, Akaydın G, Yesilada E. Investigation of in vitro opioid receptor binding activities of some Turkish Salvia species. Res Nat Prod. 2011;5:281-289.
18. Kafi M, Lahuti M, Zand E, Sharifi HM, Goldani M. Plant Physiology, Mashhad University Jahad Publications, Mashhad, Iran. 2000.
19. Reddy PCO, Sairanganayakulu G, Thippeswamy M, Reddy PS, Reddy MK, Sudhakar CH. Plant Sci.2008;175:372-384.
20. Jaleel CA, Gopi R, Panneerselvam R. Plant Omics J. 2009;2:30-40.
21. Hsaio TC. Annual Review of Plant Physiology. 1973;24:519-570.
22. Kuchaki A, Nasiri Mahallati M. Crop Ecology, vol 1, Gutenberg Publications, Tehran, Iran. 1992.
23. Sarmadnia G. In the Proceedings of the 1st Iranian Congress of Crop Sci, Karaj, Iran, pp. 1993;157-169.
24. Hughes SG, Bryant JA, Smirinoff N. Molecular biology, application to studies tolerance. In: Plants under stress, GJ Hamlyn, TJ flowers and MB Jones, Cambridge university press, New York, pp. 1989;131-135.
25. Stephanie EB. VP Svoboda, AT Paul and WVI Marc, Soc Horticulture, 2005;130:775-781.
26. Lebaschi MH, Sharifi Ashoorabadi E. Iranian J Med Aromat Plants. 2004;20:249-261.
27. Sangwan NS, Farooqi Abad AH, Sangwan RS. Newphytologist. 1994;128:173-179.
28. Ardakani MR, Abbaszadeh B, Sharifi Ashoorabadi E, Lebaschi MH, Paknejad F. Iranian J Med Aromat Plants. 2007;23:251-261.
29. Abbaszadeh B, Sharifi Ashoorabadi A, Lebaschi MH, Haji Bagerkandi MN, Moghadami F. Effect of drought stress on proline, soluble sugars, chlorophyll and relative water content of Melissa officinalis L. Iranian J Med Aromat Plants. 2007;23:504 -513.
30. Omidbaigi R, Sefidkon F, Sadrai K. Effect of sowing dates on the aeeantial oil content and composition of foeniculum vulgare cv. ' Soroksari' a potential source of anethole, Euro Cosmetics. 2004;12:34-36.
31. Said-Al Ahl HAH, Hasnaa Ayad Hendawy SF. Effect of potassium humate and nitrogen fertilizer on herb and essentia oil of oregano under different irrigation intervals, Ozean J App Sci. 2009;2:319-323.
32. Fretz TA. Effect of photoperiod and nitrogen on the composition of Juniperus horizontalis Moench cv. ' plmosa', J Am Soc Hortic Sci. 1976;101:611-613.
33. Gunes A, Inal A, Alpuslan M, Fraslan F, Guneri E, Cicek N. Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize grown under stress. J Plant Physiol. 2007;164:728-736.
34. Irrigoyan JJ, Emerich DW, Sanchez-Diaz M. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plantarum.1992;84:55-60.