Effects of Methyl Jasmonate and Chitosanon Shoot and Callus Growth of Iranian Hypericum perforatum L. in vitro Cultures

Authors

1 Department of Horticultural Science, Tehran Universitty, Karaj, Iran.

2 Department of Horticultural Science, Ferdowsi University of Mashhad, Mashhad, Iran.

3 Research and Education Center for Agriculture and Natural Resource of Fars Province, Shiraz, Iran.

Abstract

The present study was investigated the effects of two elicitors methyl jasmonate (0, 125, 250 and 500 µM) and chitosan (0, 50, 150 and 250 mg L-1) on shoot and callus growth of Iranian Hypericum perforatumL. in vitro cultures in separate experiments. The experiment was done in the base of completely randomized design with five replications and five explants in each replication. Methyl jasmonate significantly reduced the regeneration of cultured shoots and developed shoots necrosis as well as the growth of treated calluswith methyl jasmonate was ceased. In spite of methyl jasmonate, chitosanwas more effective in stimulating the growth of cultured explants. Chitosan significantly increase the regeneration of H. perforatum shoots and the highest regenerated shoots number (9.29 shoots per explant) and shoot weight (1.82 g) were obtained in 250 mg L-1chitosan. The growth of callus in response to chitosan was higher than control treatment and the treated calli with 250 mg L-1 chitosan showed the highest diameter and weight. The essential oil content and chemical compositions of in vitro proliferated Iranian H. perforatum shoots were also investigated. The essential oil content was 0.02% (w w-1). The characterization of essential oil by GC and GC/MS analyses, allowed the identification of 35 volatile constituents, accounting for 97.01% of the total oil composition and sesquiterpenes (51.72%) represented by γ–gurjunene (22.34%) were the main compounds.

Keywords

References

1. Robson NKB. Studies in the genus Hypericum L. (Guttiferae) 4(2).Section 9. Hypericum sensulato (part 2): subsection 1. Hypericum series 1. Hypericum. Bull Nat Hist Mus London Bot. 2002;32:61-123.
2. Kirakosyan A, Sirvent TM, Gibson DM, Kaufman PB.The production of hypericins and hyperforin by in vitro cultures of St. John’s wort (Hypericum perforatum). Biotech Appl Biochem. 2004;39:71-81.
3. Smelcerovic A, Verma V, Spiteller M, Ahmad SM, Puri SC, Qazi GN. Phytochemical analysis and genetic characterization of six Hypericum species from Serbia. Phytochem. 2006;67:171-177.
4. Charchoglyan A, Abrahamyan A, Fujii I, Boubakir Z, Gulder TAM, Coste A, Vlase L, Halmagy A, Deliu C, Coldea G. Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum. Plant Cell Tiss Organ Cult. 2011;106:279-288.
5. Gadzovska S, Maury S, Delaunay A, Spasenoski M, Joseph C, Hagege D. Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant Cell Tiss Organ Cult. 2007;89:1-13.
6. Liu XN, Zhang XQ, Sun JS. Effects of cytokinins and elicitors on the production of hypericins and hyperforin metabolites in Hypericum sampsonii and Hypericum perforatum. Plant Growth Regul. 2007;53:207-214.
7. Kutchan TM, Vardapetyan H, Bringmann G, Ebizuka Y, Beerhues L. Differential accumulation of hyperforin and secohyperforin in Hypericum perforatum tissue cultures. Phytochem. 2007;68:2670-2677.
8. Vardapetyan HR, Oganesyan AA, Kabasakalyan EE, Tiratsuyan SG. The influence of some elicitors on growth and morphogenesis of Hypericum perforatum L. callus cultures. Russian J Develop Biol. 2006;37:350-353.
9. Gadzovska S, Tusevski O, Antevski S, Atanasova-Pancevska N, Petreska J, Stefova M, Kungulovski D, Spasenoski M. Secondary metabolite production in Hypericum perforatum L. cell suspensions upon elicitation with fungal mycelia from Aspergillus flavus. Arch Biol Sci Belgrade. 2012;64:113-121.
10. Dias ACP, Francisco A, Barberian T, Ferreria F, Ferreres F. Unusual flavonoids produced by callus of Hypericum perforatum. Phytochem. 1998;48:1165-1168.
11. Shilpashree HP,Ravishankar Rai V. In vitro plant regeneration and accumulation of flavonoids in Hypericum mysorense. Int J Integr Biol. 2009;8:43-49.
12. Gudes AP. Essential oil from plants and in vitro shoot cultures of Hypericum and erosaemum L., Hypericum perforatum L. and Hypericum undulatum Schousboeex Willd. Univercidade do Minho. Available online: http://hdl. Handle. net/1822/ 9816. 2009.
13. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 1962;15:473-497.
14. Adams RP. Identification of Essential Oils by Gas Chromatography Quadruple Mass Spectroscopy. Allured Publishing Corporation, Carol Stream, USA. 2001.
15. Coste A, Vlase L, Halmagy A, Deliu C, Coldea G. Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum. Plant Cell Tiss Organ Cult. 2011;106:279-288.
16. Kim OT, Kim MY, Hong MH, Ahn JC, Hwang B. Stimulation of asiaticoside accumulation in the whole plant cultures of Centella asiatica (L.) Urban by elicitors. Plant Cell Rep. 2004;23:339-344.
17. Sivanandhan G, Rajesh M, Arun M, Jeyaraj M, Kapil Dev G, Arjunan A, Manickavasagam M, Muthuselvam M, Selvaraj N, Ganapathi A. Effect of culture conditions, cytokinins, methyl jasmonate and salicylic acid on the biomass accumulation and production of withanolides in multiple shoot culture of Withania somnifera (L.) Dunal using liquid culture. Acta Physiol Plant. 2012;1861-1664.
18. Walker TS, Bais HP, Vivanco JM. Jasmonic acid-induced hypericin production in cell suspension cultures of Hypericum perforatum L. (St. Johns wort). Phytochem. 2002;60:289-293.
19. Krzyzanowska J, Czubacka A, Pecio L, Przybys M, Doroszewska T, Stochmal A, Oleszek W. The effects of jasmonic acid and methyl jasmonate on rosmarinic acid production in Mentha piperita cell suspension cultures. Plant Cell Tiss Organ Cult. 2011;108:73-81.
20. Creelman RA, Mullet JE. Jasmonic acid distribution and action in plants: regulation during development and response to biotic and abiotic stress. Proc Natl Acad Sci U.S.A. 1995;92:4114-4119.
21. Nge KL, New N, Chandrkrachang S, Stevens WF. Chitosan as a growth stimulator in orchid tissue culture. Plant Sci. 2006;170:1185-1190.
22. AitBarka EA, Eullaffroy P, Clement C, Vernet G. Chitosan improves development and protects Vitisvinifera L. against Botrytis cinerea. Plant Cell Rep. 2004;22:608-614.
23. Kowalski B, Terry FJ, Herrera LDA.Application of soluble chitosan in vitro and in the greenhouse to increase yield and seed quality of potato minitubers. Potato Res. 2006;49:167-176.
24. Shimasaki K, Tanibuchi Y, Fukumoto Y. The effects of chitosan on organogenesis in protocorm-like body (PLB) of Cymbidium finlaysonianum L. J Society high techno agric. 2003;15:90-93.
25. Uthairatanakij A, Teixeira DA, Silva JA. Obsuwan K. Chitosan for improving orchid production and quality. Orchid Sci Biotechnol. 2007;1:1-5.
26. Tocci N, Ferrari F, Santamaria AR, Valletta A, Pasqua R. Chitosan enhances xanthone production in Hypericum perforatum subsp. Angustifolium cell cultures. Nat Prod Res. 2010;24:286-289.
27. Dornenburg H, Knorr D. Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzyme Microb Technol. 1995;17:674-684.
28. Kanchanpoom K, Phongdara A, Kanchanpoom k. The Effect of chitosan on the organogenesis of oil palm embryo-derived callus. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2010;1:213-217.
29. Algam SAE, Xie G, Li B, Yu S, Su T, Larsen J. Effects of Paenibacillus strains and chitosan on plant growth promotion and control of ralstonia wilt in tomato. Plant Pathol. 2010;92:593-600.
30. Morshedlu MR. Investigation some of native populations of Hypericum with morphological, phytochemical characters as well as molecular markers of DNA.MA thesis, Tehran University, Tehran, Iran. 2012.
31. Gudes AP, Amorim LR, Vicente AMS, Ramos G, Fernandes-Ferreira M. Essential oil from plants and in vitro shoots of Hypericum and erosaemum L. Agric Food Phytochem Anal. 2003;15:146-151.
Journal of Medicinal plants and By-Products
Volume 6, Issue 2
September 2017
Pages 165-172

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