The Effect of Rootstocks on the Sugars, Acids, Carotenoids, Chlorophylls and Ethylene of Clementine Mandarin (Citrus clementina)

Authors

1 Department of Horticulture, Roudehen Branch, Islamic Azad University, Roudehen, Iran

2 Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization, Tehran, Iran

Abstract

Investigations have demonstrated that ratio of sugars to organic acids affects the particular taste of citrus fruit and carotenoids are essential for prevent of the disease.  It seems that some rootstocks enhance the ripening of fruits and stimulatethe biosynthesis of sugars and carotenoids. The aim of this study was to evaluate the effect of rootstocks on sugars, organic acids andcarotenoids of fruit. The content of individual sugars and organic acids in fruits were determined by HPLC. Total acidity (TA), total soluble solids (TSS) and pH value of juice was also evaluated. Total carotenoid and chlorophylls content was measured using a spectrophotometer. The content of ethylene in fruits was determined by Gas chromatograph.   HPLC analysis of juice allowed to detection of 3 sugars and 2 acids.  Sucrose was the dominant sugar for all rootstocks. The amount of total sugars ranged from 102.27 (mg/ml) (Flying dragon) to 118.07 (mg/ml) (Orlando tangelo). The amount of total acids changed from 6.62 (mg/ml) (Trifoliate orange) to 8.48 (mg/ml) (Flying dragon). The amount of ascorbic acid varied from 0.44 (mg/ml) (Trifoliate orange) to 0.68(mg/ml) (Orlando tangelo).The pH value ranged from 3.65 (Flying dragon) to 3.95 (Orlando tangelo), TSS content changed from11.2 (%) (Flying dragon) to11.9 (%) (Orlando tangelo), TSS/TA varied from15.13 (Flying dragon) to 20.88(Orlando tangelo). Juice content ranged from 49.29 (%) (Trifoliate orange) to 54.47 (%) (Orlando tangelo). The amount of fruit production changed from 12 (Kg /tree) (Flying dragon) to 83(Kg /tree) (Orlando tangelo). The amount of total carotenoid varied from 0.12 (Murcott) to 0.15(Orlando tangelo, Sour orange, Flying dragon) (mg/gr DW). Among the six rootstocks evaluated, Orlando tangelo demonstrated the maximum rate of sugars, pH, TSS, TSS/TA, juice, ascorbic acid and carotenoids. As an outcome of our investigation, we can express that the rootstocks can affect the amount of sugars, acids andcarotenoids of fruit.

Keywords


1. Varnam A, Sutherland JM. Beverages: technology, chemistry and microbiology, Springer Science & Business Media, New York. 2012.

2. Karadeniz F. Main organic acid distribution of authentic citrus juices in Turkey. Turk J Agric Fore. 2004;28:267-271.

3. Preedy VR,  Watson RR,   Pate VB. Nuts and seeds in health and disease prevention, Academic Press, London. 2011.

4. Rostagno MA,  Prado JM. Natural product extraction: principles and applications, Royal Society of Chemistry, London. 2013.

 5. Paliyath G, Murr DP, Handa AK,   Lurie S. Postharvest biology and technology of fruits, vegetables and flowers, John Wiley & Sons, New York. 2009.

6.Rees D,  Farrell G,  Orchard J. Crop post-harvest: science and technology, perishables. Vol 3, John Wiley & Sons, Uk . 2012.

7. Barry GH, Castle WS, Davies FS. Rootstocks and plant water relations affect sugar accumulation of citrus fruit via osmotic adjustment. J Am Soc Hortic Sci. 2004;129:881-889.

8. Bermejo A, Cano A. Analysis of nutritional constituents in twenty citrus cultivars from the Mediterranean area at different stages of ripening. Food Nutr Sci. 2012;3:639-650.

9. Legua P, Fornerb JB, Hernandeza FCA, Forner-Giner MA. Total phenolics, organic acids, sugars and antioxidant activity of mandarin (citrus clementina Hort. ex Tan.) variation from rootstock. Sci Hort. 2014;174:60-64.

10. Mashayekhi K,   Sadeghi H,   Akbarpour V,   Atashi S,   Mousavizadeh SJ,  Abshaei M,   Nazari Z. Effect of some citrus rootstocks on the amount of biochemical composition of Parson Brown and Mars oranges in Jiroft.  J Hortic Sci. 2013;27:9-17.

11. Van-Wyka AA, Huysamera M, Barry GH. Extended low-temperature shipping adversely affects rind colour of ‘Palmer Navel’ sweet orange [Citrus sinensis (L.) Osb.] due to carotenoid degradation but can partially be mitigated by optimising post-shipping holding temperature. Postharvest Biol Technol. 2009;53:109–116.

12. Kumar D, Singh BP, Kumar P. An overview of the factors affecting sugar content of potatoes. Ann Appl Biol. 2004;145:247-256.

13. Salter A,  Wiseman H, Tucker G.  Phytonutrients, John Wiley & Sons. New York. 2012.

14. Maloney VJ, Park JiY, Unda F, Mansfield SD. Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation. J Exp Bot. 2015;1-12.

15. Roa AR, Garcia-Luis A, Barcena JLG, Huguet CM. Effect of 2,4-D on fruit sugar accumulation and invertase activity in sweet orange cv. Salustiana.Aust J Crop Sci. 2015;9:105-111.

16. Tomala K, Andziak J, Jeziorek K, Dziuban R. Influence of rootstock on the quality of 'Jonagolg' apples at harvest and after storage . J Fruit Ornam Plant Res, 2008; 16:31-38.

17. Yang J, Guo L. Biosynthesis of β-carotene in engineered E. coli using the MEP and MVA pathways.Microb Cell Fact. 2014;13:1-11.