Genetic and Morphological Diversity of Wild Mint "Mentha longifolia (L.) Hudson subsp. noeana (Briq.) Briq." in South and Southeastern Iran

Authors

Department of Agriculture, Faculty of Agriculture and Natural Sources, University of Hormozgan, Bandar Abbas, Iran

Abstract

The wild mint "Mentha longifolia (L.) Hudson subsp. noeana (Briq.) Briq." is an important medicinal plant which contains several worthy components like: menthone, pulegone, pipertenon, menthol, thymol, and carvone in its essential oil. This plant grows extensively in center of Asia including Iran. Because of so worthy components and its big role in traditional and modern medicine, the wild mint diversity was considered based on morphological and phytochemical variables and DNA markers (RAPD and ISSR) in South and Southeastern Iran. A total of 64 genotypes of M. longifolia  subsp. noeana genotypes were col­lected from 8 distribution centers. In total, the 15 morphological and pigments variables were measured and calculated. The cluster analysis based on morphological and phytochemical variables divided populations in the 4 main groups. The fifteen RAPD primers with reproducible and score able amplifications characters were selected. Cluster analysis based on RAPD markers revealed seven main clusters in genetic distance 0.22. The nine ISSR primers with score able amplifications characters were selected. Cluster analysis based on ISSR markers revealed five main clusters in genetic distance 0.23. The results of this research showed enough genetic diversity among and inside of the studied wild mint populations which could be applied in following breeding and gene bank conservation programs.

Keywords

References

1. Gulluce M, Sahin F, Sokmen M, Ozer H, Daferera D, Sokmen A, Polissiou M, Adiguzel A, Ozkan H. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. Longifolia. Food Chem. 2007;103:1449-1456.
2. Iscan G, Kirimer N, Kurkcuoglu M, Baser KH,  Demirci F. Antimicrobial screening of Mentha piperita essential oils. J Agric. Food Chem. 2002;50:3943-46.
3. Ertas A, Gören AC, Hasimi N, Tolan V, Kolak U. Evaluation of Antioxidant, Cholinesterase Inhibitory and Antimicrobial Properties of Mentha longifolia subsp. noeana and Its Secondary Metabolites. Rec Nat Prod. 2015;9:105-115.
4. Sharopov FS, Sulaimonova VA, Setzer WN. Essential oil composition of Mentha longifolia from wild populations growing in Tajikistan. JMAP. 2012;1:76-84.
5. Mikaili P, Mojaverrostami S, Moloudizargari M, Aghajanshakeri S. Pharmacological and therapeutic of Mentha Longifolia L. and its main constituent, menthol. Anc Sci Life. 2013;33:131-138.
6. Loveless MD, Hamrick JL. Ecological determinants of genetic structure in plant populations. Annu Rev Ecol Syst. 1984;15:65-95.
7. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990;18:6531-6535.
8. Mohammadi F, Sharifi-Sirchi GR, Samsampour D. Morphological, genetic and pigment diversity of Nerium indicum Mill in Iran. Cell Mol Biol. 2017;63:64-71.
9. Powell W, .Morgante M, Andre C, Vogel J, Tingey S, Rafalski A. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed. 1996;2:225-238.
10. Rohlf FJ. On applications of geometric morphometrics to studies of ontogeny and phylogeny. Syst Biol. 1998;47:147-158.
11. Peakall R, Smouse PE. GeneAlex 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics. 2012;28::2537-2539.
12. Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM. PopGene32, Micro­soft Windows-based freeware for population genetic analysis, ver­sion 1.32. Molecular Biology and Biotechnology Center. University of Alberta, Canada. 2000.
13. Zeinali H,  Arzani A,  Razmjo K. Morphological and essential oil content diversity of Iranian mints (Mentha spp.). IJST. 2004;28:1-9.
14. Ibrahim HMM. Assessment of genetic diversity and relationships of five mentha species using RAPD marker. Curr Sci Int. 2017;6:271-277.
15. Kabir S, Tahira R, Fareed Khan M, Naeemullah M, Jamal A. Molecular characterization of genetic diversity of mint gene pool through RAPD Markers. IJAIR. 2014;2:576-580.
16. Sabboura D, Yacoub R, Lawand S. Assessment of genetic relationships among mint species.  Int J Chem Tech Res. 2016;9:462-468.
Journal of Medicinal plants and By-Products
Volume 7, Issue 1
April 2018
Pages 105-115

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