Antiradical, Antibacterial and Oxidative Stability of Cinnamon Leaf Oil Encapsulated in β-cyclodextrin

Authors

Centro de Investigación en Alimentación y Desarrollo, AC, Carretera a la Victoria km. 0.6. Apartado Postal 1735, Hermosillo, Sonora, Mexico (83000)

Abstract

Safety and quality of food have become a challenge for the food industry looking to replace synthetic preservatives with natural agents. In this context, cinnamon leaf essential oil (CLO) showed to be potent antioxidant and antimicrobial agent; however, its active compounds are highly reactive volatiles and grant strong odors and flavors when used as food additive. With this in mind, the objective of this study was to evaluate the antiradical and antimicrobial activity, and oxidative stability of CLO encapsulated within β-cyclodextrin (β-CD). Radical scavenging activity of encapsulated CLO measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and trolox equivalents antioxidant capacity (TEAC) methods showed values of 67.3 mol (Trolox equivalent) TE/g and 177.2 mol TE/g of sample, respectively. In addition, the total phenol and flavonoid contents were 48.7 mg (eugenol equivalents) EE/g of capsules and 8.6 mg CE/g of capsules, respectively. The antibacterial activity of the encapsulated oil against Escherichia coli, Salmonella enterica subsp. enterica serovar Choleraesuis, Listeria monocytogenes and Staphylococcus aureus showed a minimum inhibitory concentration of 5.5 mg/mL for all the tested bacteria. β-CD protected the bioactive properties of the essential oil exposed to ozone, compared to the free oil. Based on these results, the encapsulation of CLO in β-CD can be considered as a viable method for stabilizing its bioactive compounds.

Keywords

References

1. Magkos F, Arvaniti F, Zampelas A. Organic Food: Buying More Safety or Just Peace of Mind? A Critical Review of the Literature. Crit Rev Food Sci Nutr. 2006;46:23-56.
2. Witkowska AM, Hickey DK, Alonso-Gomez M, Wilkinson MG. The microbiological quality of commercial herb and spice preparations used in the formulation of a chicken supreme ready meal and microbial survival following a simulated industrial heating process. Food Control.  2011;22:616-625.
3. Barrett DM, Beaulieu JC, Shewfelt R. Color, Flavor, Texture, and Nutritional Quality of Fresh-Cut Fruits and Vegetables: Desirable Levels, Instrumental and Sensory Measurement, and the Effects of Processing. Crit Rev Food Sci Nutr. 2010;50:369-389.
4. Ribeiro-Santos R, Andrade M, Sanches-Silva A & de Melo NR. Essential oils for food Application: Natural substances with established biological activities. Food Bioprocess Tech. 2018;11:43-71.
5. Ayala-Zavala JF, González-Aguilar GA, Del-Toro-Sánchez L. Enhancing Safety and Aroma Appealing of Fresh-Cut Fruits and Vegetables Using the Antimicrobial and Aromatic Power of Essential Oils. J Food Sci. 2009;74:R84-R91.
6. Singh, G, Maurya S, deLampasona MP, Catalan CAN. A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. Food Chem Toxicol. 2007;45:1650-1661.
7. Melgarejo-Flores BG, Ortega-Ramírez LA, Silva-Espinoza BA, González-Aguilar GA, Miranda MRA, Ayala-Zavala JF.Antifungal protection and antioxidant enhancement of table grapes treated with emulsions, vapors, and coatings of cinnamon leaf oil. Postharvest Biol Tech. 2013;86:321-328.
8. Du WX, Olsen CW, Avena-Bustillos RJ, McHugh TH, Levin CE, Friedman M. Effects of Allspice, Cinnamon, and Clove Bud Essential Oils in Edible Apple Films on Physical Properties and Antimicrobial Activities. J Food Sci. 2009;74:M372-M378.
9. Antunes MDC, Cavaco AM. The use of essential oils for postharvest decay control. A review. Flavour Fragr. J. 2010;25:351-366.
10. Ponce-Cevallos PA, Buera MP, Elizalde BE. Encapsulation of cinnamon and thyme essential oils components (cinnamaldehyde and thymol) in β-cyclodextrin: Effect of interactions with water on complex stability. J Food Eng. 2010;99:70-75.
11. dos Santos NST, Athayde Aguiar AJA, de Oliveira CEV, Veríssimo de Sales C, de Melo e Silva S, Sousa da Silva R, Stamford TCM, de Souza EL. Efficacy of the application of a coating composed of chitosan and Origanum vulgare L. essential oil to control Rhizopus stolonifer and Aspergillus niger in grapes (Vitis labrusca L.). Food Microbiol. 2012;32:345-353.
12. Ayala-Zavala JF, Soto-Valdez H, González-León A, Álvarez-Parrilla E, Martín-Belloso O, González-Aguilar GA.Microencapsulation of cinnamon leaf (Cinnamomum zeylanicum) and garlic (Allium sativum) oils in β-cyclodextrin. J Incl Phenom Macrocycl Chem. 2008;60:359-368.
13. Hill LE, Gomes C, Taylor TM.Characterization of beta-cyclodextrin inclusion complexes containing essential oils (trans-cinnamaldehyde, eugenol, cinnamon bark, and clove bud extracts) for antimicrobial delivery applications. LWT-Food Sci Technol. 2013;51:86-93.
14. Ayala-Zavala JF, Gonzalez-Aguilar GA, Alvarez-Parrilla E, De la rosa LA, Vega-Vega V. Encapsulación de antioxidantes en la industria de alimentos. In: Álvarez-Parrilla GA, De la Rosa, Ayala-Zavala, Juárez, (eds.) Antioxidantes En Alimentos y Salud. 2012;527-552. Clave editorial, México
15. Singleton VL, Rossi JA. Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. J Enol Vitic. 1965;16:144-158.
16. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999;64:555-559.
17. González-Aguilar GA, Villegas-Ochoa MA, Martínez-Téllez MA, Gardea AA, Ayala-Zavala, JF. Improving Antioxidant Capacity of Fresh-Cut Mangoes Treated with UV-C. J Food Sci. 2007;72:S197-S202.
18. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26:1231-1237.
19. Vega-Vega V, Silva-Espinoza BA, Cruz-Valenzuela MR, Bernal-Mercado AT, González-Aguilar GA, Vargas-Arispuro I, Corrales-Maldonado CG, Ayala-Zavala JF. Antioxidant enrichment and antimicrobial protection of fresh-cut mango applying bioactive extracts from their seeds by-products. Food Nutr Sci. 2013;4:197.
20. Gordon R, Ryan Q, Steven M. High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics Using a FLUOstar Omega. 2009. http://www.bmglabtech.com/db_assets/applications/downloads/applications/189-tea-tree-oil.pdf
21. Baranyi J, Roberts TA, McClure P. A non-autonomous differential equation to modelbacterial growth. Food Microbiol. 1993;10:43-59.
22. Gurol MD, Vatistas R. Oxidation of phenolic compounds by ozone and ozone + u.v. radiation: A comparative study. Water Research. 1987;21:895-900.
23. Brewer MS. Natural Antioxidants: Sources, Compounds, Mechanisms of Action, and Potential Applications. Compr Rev Food Sci F. 2011;10:221-247.
24. Astray G, Gonzalez-Barreiro C, Mejuto JC, Rial-Otero R, Simal-Gándara J. A review on the use of cyclodextrins in foods. Food Hydrocolloids. 2009;23:1631-1640.
25. Tajkarimi MM, Ibrahim SA, Cliver DO. Antimicrobial herb and spice compounds in food. Food Control. 2010;21:1199-1218.
26. Bhandari BR, D'Arc BR, Thi Bich LL. Lemon Oil to β-Cyclodextrin Ratio Effect on the Inclusion Efficiency of β-Cyclodextrin and the Retention of Oil Volatiles in the Complex. J Agric Food Chem. 1998;46:4:1494-1499.
27. Ceylan E, Fung DYC. Antimicrobial activity of spices. J Rapid Meth Aut Mic. 2004;12:1-55.
28. Sánchez-González L, Vargas M, González-Martínez C, Chiralt A, Cháfer M. Use of Essential Oils in Bioactive Edible Coatings: A Review. Food Eng Rev. 2011;3:1-16.
29. Weissinger WR, McWatters KH, Beuchat LR. Evaluation of Volatile Chemical Treatments for Lethality to Salmonella on Alfalfa Seeds and Sprouts. J Food Prot. 2001;64:442-450.
30. Pettersen EF, Goddard TD, Huang CC, Couch GS, GreenblattDM, Meng EC, Ferrin TE UCSF Chimera a visualization system for exploratory research and analysis. J Comput Chem. 2004;25:1605–1612.
Journal of Medicinal plants and By-Products
Volume 8, Issue 2
September 2019
Pages 115-123

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