Effect of Seed Priming on the Enhancement of Seedling Traits in two Species of Anthemis L. Preserved in Medium and Long-term Storage and Accelerated Aged Seeds

Authors

1 Gene bank Group, Research Institute of Forests and Rangelands Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

2 Department of Rangland Research Institute of Forests and Rangelands Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

Abstract

The genus AnthemisL. (Asteraceae) as medicinal plants are used both for pharmaceutical purposes and in folk medicine. In order to study of seed priming effects on seedling growth of two species of Anthemis spp., an factorial experiment based on randomized complete design with three replications was conducted under greenhouse conditions in Research Institute of Forests and Rangelands in 2014-2015.A factorial experiment consisting three factors: 1) two species including Anthemis tinctoriaL. and Anthemis triumfettii (L.) DC. which formed the three levels of factor A, and 2) five conservation methods including: medium-term storage (active cold room 4 °C for 10 years), long- term storage (basic cold room-18 °C for 10 years), regenerated seeds in open air 22 °C for 2 years (Control) and aged seed under accelerated ageing )40 °C,98% of Relative humidity) for 48 and 72h made up the five levels of factor B, and 3) five priming treatments were including: Control (without priming), osmopriming (PEG -0.3Mpa), hormonal priming (Giberlic acid 250 and 500 mg/L), hydropriming (imbibitions with distilled water) were levels of factor C. Data collected for seed emergence percent, root and shoot length, seedling length, vigor index, seedling weight and three proxidase, catalaz and super oxid desmotaz (SOD) enzymatic activities. Result of analysis of variance showed  that effects of species, conservation and priming and their interaction were significant  for many of seedling traits and enzymatic activities. According to the results, the higher values of seedling emergence, vigor index, seedling length were obtained in A. tinctoria. In contrast, peroxidase and SOD enzymatic activity were the higher in A. triumfettii. All species had higher seedling growth by using osmopriming. Both  osmo and hormonal priming method were effective in recovery of deteriorated seeds. The mean of all traits of three species were higher in base cold room (-18 °C) than active cold room (4 °C) and this a sign effect of low temperature on seed viability. The root length were higher in accelerated ageing test (48h and 72). It was due to positive effect of priming on improvement of deteriorated seed by increasing root length. The more seed emergence characteristics were obtained with effect of osmopriming (PEG 0.3Mpa), and, hormonal priming (Gibberellic acid 250 mg/L). Regarding to result of this research work, It was proved that two priming technique osmopriming using (Poly ethylene glycol) and hormonal priming (Gibberellic acid ) were effective method for improvement of aged seed of  Anthemis spp.

Keywords

References

1. Funk VA. “Asteraceae”. -In: The Families and Genera of Vascular Plants. (ed. Kubitzki, K.) Springer Verlaf. 2007;8:175-180.
2.Yao ZL, Liu F, Gao C, Rampitsch DM, Reinecke JA, Ozga B, Ayele T. Developmental and seed aging mediated regulation of ant oxidative genes and differential expression of proteins during pre and post germinative phases in pea. J Plant Physiol. 2012;169:1477-1488.
3. Uzel A, Guvensen A, Cetin E. Chemical composition and antimicrobial activity of the essential oils of Anthemis xylopoda O. Schwarz from Turkey. J Ethnopharmacol. 2004;95:151-54.
4. Mozaffarian V. A Dictionary of Iranian Plant Names. Tehran: Farhang Moaser Pub. 2007 (In Persian).
5. Halevy AH, New flower crops. In: Janick J, editore. Perspectives on new crops and new uses. ASHS, Press, Alexandria, VA. P. 1999;407-409.
6. Fennell CW, Light ME, Sparg SG. Stafford GI, Van Staden J. Assessing African medicinal plants for efficacy and safety: agricultural and storage practices. J Ethnopharmacol. 2004;95:113–121.
7. FAO. Draft gene bank standards for plant genetic resources for food and agriculture. 2013. http://www.fao.org/agriculture/crops/core themes/theme/seeds-pgr/conservation/gbs/en/ (accessed April 2013).
8. Royal Botanic Gardens Kew Seed Information Database (SID). 2008. Version 7.1. http://data.kew.org/sid/
9. Probert RJ. The role of temperature in the regulation of seed dormancy and germination. In Fenner M, editor. , ed., Seeds: The Ecology of Regeneration in Plant Communities, Ed 2 CAB International, Wallingford, UK, pp. 2000;261–292.
10. Butler LH, Hay FR, Ellis RH, Smith RD, Murray TB. Priming and re-drying improve the survival of mature seeds of Digitalis purpurea during storage. Ann Bot. 2009;103:1261-1270.
11. Nawaz J, Hussain M, Jabbar A, Nadeem GA, Sajid M, Subtain M, Shabbir I. Seed Priming A Technique Inter J Agric Crop Sci. 2013;6:1373-1381.
12. Taylor AG, Allen PS, Bennett MA, Bradford KJ, Burrisand JS, Misra MK. Seed enhancements. Seed Sci Res. 1998;8:245-256.
13. Hampton, J. G., Brunton, B. J., Pemberton, G. M. and Powarth, J. S. Temperature and time variables for accelerated again Vigor testing of Pea seed. Seed Sci and Technol. 2004;32:261-264.
14. Chiu KY, Sung JM. Effect of priming temperature on storability of primed sh-2sweet corn seed. Crop Sci. 2002;42:1996-2003.
15.  ISTA, Handbook on Seedling Evaluation. Int Seed Testing Assoc. Zurich. 2008;519.
16. Lekh R, Khairwal IS. Evaluation of pearl millet hybrids and their parents for germ inability and field` emergence. Indian J Plant Physiol. 1993;2:125.
17. Abdual-baki, AA, Anderson, JD. Relationship between decarboxylation of glutamic acid and vigor in soybean seed. Crop Sci. 1973;13:222-226.
18. Shannon LM, Kay E, Law JY.  Peroxidase isoenzyme from horse radish roots: isolation and physical properties. J Biol Chem. 1966;241:2166–2172.
19. Aebi H.  Catalase in vitro. Methods Enzymol. 1984;105:121–126.
20. Giannopolitis CN, Ries SK. Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol. 1977;59:309-314.
21. Rincker CM. Germination of forage crop seeds after 20 years of subfreezing storage. Crop Sci. 1995; 22:229-231.
22. Priestley DA. Seed aging. Cornell University Press. 1986.
23. Alizadeh MA. Evaluation of percentage of germination, total speed of germination and vigor index of 17 medicinal plants species to ageing test. Proceeding of National Congress in Sustainable Development of Medicinal Plants, (NCSDMP), 27-29 July 2005 Mashhad. 2005;171-172.
24. Tekrony DM. Accelerated aging. In: Van de venter, H.A. (Ed.) Seed vigor testing seminar. Copenhagen: ISTA. pp. 1995;53-72.
25. MacDonald MB. Seed deterioration: physiology, repair and assessment. Seed Sci. Technol. 1999; 27:177-237.
26. Marshal  AH, Lewis DN. Influence of seed storage conditions on seedling emergence, seedling growth and dry matter production of temperate forage grasses. Seed Sci Technol. 2004;32:493-501.
27. Falahhosseini L, Alizadeh MA,  Vazan S. Priming  Effect of seed priming on the enhancement of germination traits of aged seeds of Chamomile (Matricaria chamomilla L.) preserved in medium and long-term storage.J Med Plants By-products. 2017;1:1-9.
28. Arteca NR. Plant Growth Substances: Principles and Applications. Springer, Publication. 1995;146.
29. Sajjadi Jaghargh s, Alizadeh MA, Kalagari M. Effect of Osmopriming, Hydropriming and Pre-chilling on Seed Emergence Enhancement and Seedling Vigor of four Medicinal Species of Anthemis under Greenhouse Conditions,  Bulletin UASVM Horticulture.  2014;71:74-84.
30. El-Araby M, Hegazi AZ.  Response of tomato seed to hydro and osmopriming: and possible relation of some antioxidant enzymes and endogenous polyamine fractions. Egyptian Journal of Biology. 2004;6:81-93.
31. Rasoolzadeh L, Salehi Shanjani P, Madani H. Effect of Priming on Germination and Enzyme Activity of Achillea vermicularis Seeds after Naturally and Accelerated Aging. J Med Plants By-products. 2017;1:11-16.
32. Burguieres E, McCu P, Kwon Y, Shetty K. Effect of vitamin C and folic acid on seed vigor response and phenolic-linked antioxidation activity. Bioresource Technol. 2007;98:1393-1404.
33. Jie L, Gong She L, Dong Mei O, Fang Fang L, En Hua W. Effect of PEG on germination and active oxygen metabolism in wildrye (Leymu.7 Chinensis) seeds. Acta Prataculturae Sinica. 2002;11:59-64.
Journal of Medicinal plants and By-Products
Volume 8, Issue 2
September 2019
Pages 153-162

Files

Share

How to cite

Statistics