Micro Morphological Study of Several Populations of Zataria multiflora Boiss.


1 Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

2 Faculty of Natural Sciences of Tabriz University, Tabriz, Iran


Zataria multiflora Boiss. is  one of the most important species of the Lamiaceae family, which has numerous medicinal properties in traditional medicine, and is related to the thyme species in terms of botanical features and composition of essential oil. In this research, the anatomical characteristics of vegetative organs including leaves, inflorescence, stems and petioles of 4 populations of shirazian thyme have been collected from different areas of Iran and were investigated and compared. Methods include making hand-cuts and observations with an optical microscope. The results of this study showed that there is a similarity to the characteristics of theinflorescence, stem, leaf and petiole in the populations of shirazian thyme. In terms of stem and leaf diameter, the thickness of the cork and xylem, frequency and type of non-glandular trichomes and glandular trichomes there are differences between the studied populations.  The results indicate that the study of anatomical characters is an appropriate method for identification of populations of a species and the role of ecological factors on the development of the internal structures of plants could be studied too.


1. Jamzad Z, Flora of Iran Lamiaceae  pub Research Institute of Forest and Rangelands. 2012;76.

2. Sajed H, Sahebkar A, Iranshahi M. Zataria multiflora Boiss. (Shirazi thyme)- an ancient condiment with modern pharmaceutical uses. J Ethnopharmacol. 2013;145:686-698.

3. Albouyeh RM, Mirzaie-Nadoushan H, Majd A, Rezaie MB, Investigation of several anatomic characteristics in menthe species. Iranian Rangelands and Forest Plant Breeding and Genetic Research. 2002;8:81-92.

4. Abbas Azimi R, Jamzad Z, Sefidkon F, Bakhshi-Khaniki S, The potential value of phytochemical and micromorphological characters in taxonomic treatment of genus Vitex L. Lamiaceae. Iranian J Botany. 2006;12:15-35.

5. Satil F, Kaya A. Leaf anatomy and hairs of Turkish Satureja L. (Lamiaceae). Actabiologica Cracoviensia series Botanica. 2007;49:67-76.

6. Nejadsattari T, Hamdi S. M. M, Heidarian F, Assadi M. Anatomical studies of leaf and stem on Ornithogalum (Hyacinthaceae) in Iran.Taxonomy and Biosystematics. 2013;15:49-66.

7. Güven S, Beyazoğlu O, Makbul S, Türkmen Z, Kandemir A. Anatomica features of six Onosma L. (Boraginaceae) species From Turkey. Iranian J Botany. 2013;19:94-103. Tehran.

8. Zarinkamar  F, Marzban A. Effect of altitude on anatomy of Plantago major and Plantago lanceolata. Iranian J Biology. 2009;23:532-540.

9. Azarnivand H, Jafari  M , Zarechahoki M. A, Rabiee M. Anatomical characteristics of Artemisia sieberi (Case study: Vard-Avard, Garmsar, Semnan). Pajouhesh  Sazandgi. 2011;80:100-108.

10. Makbul S, Beyazoğlu O. Morphological and anatomical studies on some Scrophularia L. (Scrophulariaceae) taxa from NE Anatolia, Turkey. Iranian J Botany. 2010;30:186-195.

11. Seifi Z, Zarinkamar F. Anatomical studies of some Avena species in Iran. Rostaniha. 2006;8:186-203.

12. Hamdi SMM, Assadi M, Iranbakhsh A, Malekloo M, Ebadi  M. Anatomical studies on leaf on Typhaceae in Iran. Taxonomy & Biosystem. 2012;11:13-22.

13. Mehrabian AR, Azizian D, Zarre Sh, Podlech D. Petiol anatomy in Astragalus Sect. Incani DC. (Fabaceae) in Iran. Iranian J Botany. 2007;13:138-145.

14. Zarrinkamar F, Jalili A, Hamzehee B, Asri Y, Hodgson J G, Thompson K, Shaw S. Foliar anatomy of Carex in Astragalus, NW. Iran. The Iranian J Botany. 2002;9:261-266.

15. Ramazannejad Ghadi R, Azizian D, Assadi, M. Comparative anatomical analysis of stem in four genera of the tribe Salsoleaa, Chenopodiaceae. Iranian J Botany. 2006;12:169-182.

16. Habibi M, Azizian D, Sheidai M, Khatamsaz M. Comparative anatomical studies of some Clematis L. (Ranunculaceae) species in Iran. J Sci. 2008;2:20-29

 17. Amini Rad M, Hroudová Z. Leaf and stem anatomy in Iranian Bolboschoenusspecies(Cyperaceae), as related to their taxonomy and ecology. Iranian J Botany. 2013;19:221-234. Tehran.

18. Keshavarzi M, Esfandani Bozchaloyi S.Leaf and stem comparative anatomical analysis of three genera of Alsinoideae (Caryophyllaceae). Iranian J Botany. 2014;20:71-79. 

19. Hosseini SZ, Rahiminejad MR, Saeidi H. Leaf anatomical structure of Iranian narrow-leaved species of the genus Festuca L. (Poaceae, Poeae). Iranian J Botany. 2013;10:86-93. Tehran

20. Seyedi N, Jalali S. Gh, Moghaddam M. Leaf anatomical investigation of Pistacia atlanticavarkurdica. Journal of Plant Biology. 2013;16:71-80.

21. Rabie  M, Asri Y, Abbas Azimi R, Dehgan M. The effects of climatical parameters on anatomical structure of Artemisia sieberiBesser populations. Iranian J plant biol. 2012;4:57-70.

22. Najafpour Navaei  M, shariat  A. A study on ionomic indices of Zataria multiflora Boiss. an Iranian native medicinal plant.  Iranian J Medicinal & Aromatic Plants. 2017;33:812-819.

23. Makbul  S, Türkmen Z, Kandemir A, Beyazoglu O. Morphological anatomical and palynological properties of endemic Onosma sintenisiiHausskn. & Bornm. (Boraginaceae). Herb J Systematic Botany. 2008;15:125-136.

24. Makbul S, Coskuncelebi K, Türkmen Z, Beyazoglu O. Comparison of foliar anatomy of Scorzonera L. (Asteraceae) taxa from northeast Anatolia. Pakistan J Botany. 2011;43:135-155.

25. Ismaili S, Roohi V, Shiran B, Mohammadkhani AB,Effects of Calcium Chloride, Gibberellin and Benzyladenine on Qualitative and Quantitative Characteristics and Flower Longevity of Zinnia (Zinnia elegans). J Hortic Sci. 2014;4:444-452.

26. Fahn  A, Structure and function of secretory cells In Hallahon DL, and Gray JC [eds.]. Advances in botanical research. Incorporating advances in plant pathology. Plant trichomes. Academic Press, London. 2000;31:37-75.

27. Bisio A, Corallo A, GastaldoP, Romussi G, Ciarallo G, Fontana N, Tommasi ND, and Profumo P. Glandular hairs and secreted material in Salvia blepharophyllaBrandegee ex Epling Grown in Italy. Annals Botany. 1999;83:441-452.

28. Kaya A, Demirci B, Baser K.H.C. Glandular trichomes and essential oils of Salvia glutinosa L. South African J Botany. 2003;69:422-427.