Extraction and Identification of phytochemicals in Iranian oak (Quercus brantii var. Persica) Collected in Arghavan Valley, Ilam County by HS-SPME and GC-MS

Authors

1 Department of Surgery, School of Medicine, Emam Khomeini Hospital, Ilam University of Medical Sciences, Ilam, Iran

2 Department of Pediatrics, School of Medicine, Ilam University of Medical sciences, Ilam, Iran

3 Department of Medicinal Plants & By-products, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization, Tehran, Iran

4 Biotechnology and Medical Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran

5 Department of Biology and Vector Control, School of Health, Ilam University of Medical Sciences, Ilam, Iran

Abstract

In Iranian traditional medicine Quercus brantii var. Persica (Oak) is considered warm- and dry-natured. It is used for gastric pain, ulcer, anemia, hemorrhoids and rickets, burns, indigestion, diarrhea, and infection. Oak is readily available and has a variety of medicinal and health effects in both traditional and modern remedies. The main objective of this research is Identification in oak compounds, using Headspace-solid phase microextraction (HS-SPME) and Gas chromatography–mass spectrometry (GC-MS). Oak fruits were collected from Argavan Valley, Ilam, west of Iran, after drying and powdering, chemical compounds were isolated by HS-SPME and identified by using GC-MS method. The results analysis of Quercus brantii revealed the existence of 41 chemical compounds. Major chemicals included β- pinene oxide (8.65%), Tetrahydro- linalyl acetate (8.51%), 2-methoxy –p-cresol (7.65%), 2-methoxy pyrazine (5.08%), 2-acetyl pyridine, 2,3-dimethyl pyrazine (4.42%), Trans- linalool oxide (3.79%), β- pinene(3.66%), Verbenone (3.43%), and Terpin-4-ol (3.27%).

Keywords


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