Evaluation of Phytochemical, Total Phenolic and Flavonoidcontent, Antioxidant Activities, and Repelling Property of Sambucusebulus

Authors

1 Faculty of Health, Mazandaran University of Medical Sciences, Sari, Iran

2 Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran

3 Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

4 Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran

Abstract

The therapeutic and anti-infectious effects of Sambucus ebulus L. have been well documented in the traditional medicine. The purpose of this study was to identify the domestic herbal plant with insect repellent property. Leaf and fruit of Sambucus ebulus L. was collected from Kordkail Kola Sofla rural district and dried. To assess the repellent property, the concentrations of 50 mg/kg, 100 mg/kg, and 250 mg/kg) were prepared. Then 0.4 mL of each concentration was applied on an area of 4 × 6 cm on the shaved Albino (Indian pig) skin and exposed to the mosquito bite. After 30 minutes, the number of adult mosquito bites (three replications) was recorded and analyzed by ANOVA test at a probable level of 5%.The highest phenolic content in the leaf and fruit methanol extract was 155.925 mg/g and of 92.777 mg/g, respectively. Based on IC50, the highest level of antioxidant activity in the leaf and fruit methanol extract were 60.09μg/mL and 417.846μg/mL, respectively. The highest repelling property was correlated with the concentration of 250mg/kg of Sambucus ebulus. The repelling effects were 73.4% for the leaf and 78% for fruit extracts. Leaf and fruit extracts of Sambucus ebulus contained phenolic and flavonoid with strong antioxidant effects. The fruit hexane and methanol extracts were better repellent than the leaves, and the methanol extracts effect of these organs was greater.

Keywords

References

1. Khalili M, Fathi H, Ebrahimzadeh MA. Antioxidant activity of bulbs and aerial parts of Crocus caspius, impact of extraction methods. Pak J Pharm Sci. 2016;29:773-777.
2. Khoo HE, Azlan A, Kong KW, Ismail A. Phytochemicals and medicinal properties of indigenous tropical fruits with potential for commercial development. Evid Based Complement Alternat Med. 2016;20-40.
3. Shokrzadeh M, Saravi SS. The chemistry, pharmacology and clinical properties of Sambucus ebulus: A review. J med plants res. 2010;4:95-103.
4. Unsal, V. Natural Phytotherapeutic Antioxidants in the Treatment of Mercury Intoxication-A Review. Adv Pharm Bull. 2018;8:365.
5. Engwa GA. Free Radicals and the Role of Plant Phytochemicals as Antioxidants Against Oxidative Stress-Related Diseases, in Phytochemicals-Source of Antioxidants and Role in Disease Prevention. IntechOpen. Chapter 4. 2018;50-73.
6. Saha SK, Lee SB, Won J, Choi HY, Kim K, Yang GM, Dayem AA, Cho SG. Correlation between oxidative stress, nutrition, and cancer initiation. Int J Mol Sci. 2017;18:1544.
7. Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J. 2016;15:71.
8. Joseph N, Anjum N, Tripathi YC. Phytochemical screening and evaluation of polyphenols, Flavonoids and Antioxidant Activity of Prunus cerasoides D. Don Leaves. J Pharm Res. 2016;10:502-508.
9. Prabu K, Rajasekaran A, Bharathi D, Ramalakshmi S Anti-oxidant activity, phytochemical screening and HPLC profile of rare endemic Cordia diffusa. JKSUS. 2018.
10. Cvetanovic A, Djurovic S, Maskovic P, Radojkovic M, Svarc-Gajic J, Zekovic Z. Polyphenolic profile of sambucus ebulus root, leaf and fruit extracts. “xxi savetovanje o biotehnologiji. 2016;21:631-637.
11. Tasinov O, Kiselova-Kaneva Y, Ivanova D. Sambucus ebulus-from traditional medicine to recent studies. Scripta Scientifica Medica. 2013;45:36-42.
12. Khamisabadi M, Feizbakhsh A, Rahimzadeh A, Vaghef, M. comparoson of hormonal changes (NAA, IAA, GA3) of the composition of essential oil from Sambucus ebulus.  PhOL. 2018;231-239.
13. World Health Organization. Handbook for integrated vector management. 2012.
14. Yousef Mogaddam M, Motevalli Haghi F, Fazeli-Dinan M, Hosseini-Vasoukolaei N, Enayati AA. A Review of Insecticide Resistance in Malaria Vectors of Iran. JMUMS. 2016;25:394-411.
15. Ranasinghe MS, Arambewela L, Samarasinghe S. Development of herbal mosquito repellent formulations. Int J Pharm Sci Res. 2016;7:3643-3648.
16. Mandavgane SA, Pattalwar VV, Kalambe AR. Development of cow dung based herbal mosquito repellent. Nat Prod Rad. 2005;4:271-273.
17. Abu-Qare AW, Abou-Donia MB. Development of a high-performance liquid chromatographic method for the quantification of chlorpyrifos, pyridostigmine bromide, N, N-diethyl-m-toluamide and their metabolites in rat plasma and urine. J Chromatogr B Biomed Sci Appl. 2001;754:533-538.
18. Tawatsin A, Wratten SD, Scott RR, Thavara U, Techadamrongsin Y. Repellency of volatile oils from plants against three mosquito vectors. J Vector Ecol. 2001;26:76-82.
19. Prajapati V, Tripathi AK, Aggarwal KK, Khanuja SP. Insecticidal, repellent and oviposition-deterrent activity of selected essential oils against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. Bioresour Technol. 2005;96:1749-1757.
20. Negi C, Verma P.  Review on Culex quinquefasciatus: Southern House Mosquito. Int. J. Life. Sci. Scienti. Res. 2018;4:1563-1566.
21. Mozaffari S, Lahooti M, Larijani K. Total phenolic, flavonoid content and radical scavenging activity of Sambucus ebulus from Iran. Int J Res Pharm Pharmaceut Sci. 2016;1:36-38.
22. Yeşilada E, Gürbüz I, Shibata H. Screening of Turkish anti-ulcerogenic folk remedies for anti-Helicobacter pylori activity. J Ethnopharmacol. 1999;66:289-293.
23. Jabbari M, Hashempur MH, Razavi SZ, Shahraki HR, Kamalinejad M, Emtiazy M. Efficacy and short-term safety of topical Dwarf Elder (Sambucus ebulus) versus diclofenac for knee osteoarthritis: a randomized, double-blind, active-controlled trial. J Ethnopharmacol. 2016;188:80-86.
24. Schwaiger S, Zeller I, Pölzelbauer P, Frotschnig S, Laufer G, Messner B, Pieri V, Stuppner H, Bernhard D. Identification and pharmacological characterization of the anti-inflammatory principal of the leaves of dwarf elder (Sambucus ebulus). J Ethnopharmacol. 2011;133:704-709.
25. Rahimi-Esboei B, Ebrahimzadeh MA, Fathi H, Rezaei Anzahaei F. Scolicidal effect of Allium sativum flowers on hydatid cyst protoscolices. Eur Rev Med Pharmacol Sci. 2016.;20: 129-132.
26. Motevalli-Haghi SF, Ebrahimzadeh MA, Nikookar SH. Evaluating the repelling property of Juglansregia L leaf extract on the culex pipiens in invitro conditions. CMJA. 2017;6:1737-1747.
27. Fathi H, Ebrahimzadeh MA, Ahanjan M. Comparison of the antimicrobial activity of caucasian wingnut leaf extract (Pterocarya fraxinifolia) and Walnut (Juglans regia L.) plants. Acta Biologica Indica. 2015;4:67-74.
28. Ghasemi K, Bolandnazar S, Tabatabaei SJ, Pirdashti H, Arzanlou M, Ebrahimzadeh MA, Fathi H. Antioxidant properties of garlic as affected by selenium and humic acid treatments. new zeal  j crop hort. 2015;43:173-181.
29. Parekh J, Chanda S. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk J Biol. 2007;31:53-58.
30. Krishnaiah D, Devi T, Bono A, Sarbatly R. Studies on phytochemical constituents of six Malaysian medicinal plants. J MED PLANTS RES. 2009;3:067-072.
31. Yusuf AZ, Zakir A, Shemau Z, Abdullahi M, Halima SA. Phytochemical analysis of the methanol leaves extract of Paullinia pinnata Linn. Journal of Pharmacognosy and Phytotherapy.  2014;28;6:10-6.
32. Fathi H, Ebrahimzadeh MA. Antioxidant and free radical scavenging activities of Hypericum perforatum L.(st. John’s wort). IJFSE. 2013;3:68-72.
33. Barat SM, Ghalandari R. Comparative study on repellent effect of extracts and essential oils of Melissa officinalis L., Rosmarinus officinalis L. and Lavandula angustifolia Mill. against main malaria vector, Anopheles stephensi (Diptera: Culicidae). Iran J Med Aromatic Plants. 2012.
34. Ghassemi-Kahrizeh A, Safaralizadeh MH, Pourmirza AA. The effect of Bacillus thuringiensis var. tenebrionis on Colorado potato beetle larvae, Leptinotarsa decemlineata (Say)(Coleoptera: Chrysomelidae) and role of Henna as a synergist in increasing its efficiency. Iran J Agric Sci. 2003;34:539-547.
35. Ebrahimzadeh MA, Nabavi SF, Nabavi SM, Pourmorad F. Nitric oxide radical scavenging potential of some Elburz medicinal plants. Afr. J. Biotechnol. 2010;9:5212-5217.
36.  Deans CA, Behmer ST, Tessnow AE, Tamez-Guerra P, Pusztai-Carey M, Sword GA. Nutrition affects insect susceptibility to Bt toxins. Scientific reports. 2017;7:39705.
37. Hadipour A, Azizi M, NAGHDI BH, Panahandeh J, Delazar A, Aroei H. Phytochemical diversity of Eremostachys laciniata bunge populations in Iran. J Med Plants. 2016;1:9-18.
38. Krishnaiah D, Sarbatly R, Bono A. Phytochemical antioxidants for health and medicine a move towards nature. Biotechnol. Mol. Biol. Rev. 2007;2:97-104.
39. Tapondjou LA, Adler CL, Bouda H, Fontem DA. Efficacy of powder and essential oil from Chenopodium ambrosioides leaves as post-harvest grain protectants against six-stored product beetles. J Stored Prod Res. 2002;38:395-402.
40. Fathi H, Ebrahimzadeh MA, Ziar A, Mohammadi H. Oxidative damage induced by retching; antiemetic and neuroprotective role of Sambucus ebulus J Cell Biol Mol Sci. 2015;31:231-239.
41. Sathyaseelan V, Bhaskaran V. Efficacy of some native botanical extracts on the repellency property against the pink mealy bug, Maconellicoccus hirsutus (green) in mulberry crop. Recent Research in Science and Technology. 2010;2.
42. Appel AG, Gehret MJ, Tanley MJ. Repellency and toxicity of mint oil to American and German cockroaches (Dictyoptera: Blattidae and Blattellidae). J. Agric. Urban Entomol. 2001;18:149-156.
43. Jaenson TG, Garboui S, Pålsson K. Repellency of oils of lemon eucalyptus, geranium, and lavender and the mosquito repellent MyggA natural to Ixodes ricinus (Acari: Ixodidae) in the laboratory and field. J Med Entomol. 2006;43:731-736.
44. Rajkumar S, Jebanesan AJ. Repellent activity of selected plant essential oils against the malarial fever mosquito Anopheles stephensi. Trop Biomed. 2007;24:71-75.
45. Oshaghi MA, Ghalandari R, Vatandoust H, Shayeghi M, Kamallnezhad M, Tourabi KH, Abou Alhasani M, Hashemzadeh CM. Repellent effect of extracts and essential oils of Citrus limon (Rutaceae) and Melissa officinalis (Labiatae) against main malaria vector, Anopheles stephensi (Diptera: Culicidae). Iran. J. Public Health. 2003.;32:47-52.
46. Kweka EJ, Mwango'onde BJ, Mahande MJ, Mosha FW, Kimaro EE, Msangi S, Lyaruu L, Mahande AM.  Evaluation of Ocimum suave essential oil against anthropophilic malaria vector Anopheles gambiae ss after ten years of storage. Tanzan J Health Res. 2009;11.
47. Trongtokit Y, Rongsriyam Y, Komalamisra N, Apiwathnasorn C. Comparative repellency of 38 essential oils against mosquito bites. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2005;19:303-309.
48. Perez SG, Ramos-López MA, Zavala-Sánchez MA, Cárdenas-Ortega NC. Activity of essential oils as a biorational alternative to control coleopteran insects in stored grains. J MED PLANTS RES. 2010;4:2827-2835.
49. Bernays EA, Chamberlain D, McCarthy P. The differential effects of ingested tannic acid on different species of Acridoidea. Entomol Exp Appl. 1980;28:158-166.
50. Gibson DM, Greenspan Gallo L, Krasnoff SB, Ketchum RE. Increased Efficacy of Bacillus thuringiensis subsp. kurstaki m Combination with Tannic Acid. J ECON ENTOMOL. 1995;88:270-277.
Journal of Medicinal plants and By-product
Volume 9, Special
March 2020
Pages 97-105

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