Effect of Growth Regulators on Optimization of Stevia (Stevia rebaudiana) Production in vitro

Document Type : Research Paper

Authors

College of Agriculture, Islamic Azad University, Damghan Branch, Damghan, Iran

Abstract

Stevia rebaudiana (Bertoni) Bertoni importance and value have been proved in the world because of its alternative potential as natural sugar and in vitro production benefits. Tissue culture of this plant was considered in order to get the best regeneration condition. In this study, proliferation of branches and rooting were measured as a factorial based on a randomized complete block design with four replications. There were two types of shoot induction hormone treatments including BAP and Kin in 0 (control sample), 0.25 and 0.5 mg/lit concentrations and two root induction hormone treatments of IBA and NAA in vitro condition. Traits were measured after 45 days on branch, leaf and root. Results showed the most branch length and root volume with 0.25 mg/lit of Kin hormone and the most root length with 0.25 mg/lit concentration of IBA. The highest rooting was related to IBA hormone in 0.5 mg/lit concentration. According tocorrelation analysis between traits, positive and significant correlation was reported. Cluster analysis grouped shoot treatment with BAP in two 0.25 and 0.5 mg/lit concentrations in one group and Kin with 0.25 and 0.5 mg/lit concentrations in another group. Also, these treatments showed the most similarity. Also, IBA root treatment with 0.25 mg/lit concentration had the most difference in comparison with other hormone treatments. According to the HPLC results, increasing of rooting hormones concentrations (NAA and IBA) leaded to leaf Stevioside increasing. Totally, hormone treatments resulted significant differences between control and treated plants. That had positive effects on plant regeneration.

Keywords

References

1. Hossini Hashemzadeh H, Mahbali Pour N. The effect of methyl jasmonate and salicylic acid on the content of Stevioside in Stevia rebaudiana Bertoni under in vitro conditions. 13th Iran. Crop Sci Con and 3rd Iran. Seed Sci and Tech. Con. 2014.
2. Jamshidi ZA, Ghanbari AR, Sharafi Y. Bina F. Micropropagation of Stevia rebaudiana Bertoni via in vitro. 3rd Iran Agri Biot Con. 2012.
3. Das A, Gantait S, Mandal N. Micropropagation of an elite medicinal plant: Stevia rebaudiana Bert. Int J Agric Res. 2011;6:40-48.
4. Vyas SP, Dixit VK. Pharmaceutical biotechnology. CBS. 2010.
5. Ahmed MB, Salahin M, Karim R, Razvy MA, Hannan MM, Sultana R, Hossain M, Islam R. An Efficient method for in vitro clonal propagation of a newly introduced sweetener plant (Stevia rebaudiana Bertoni) in bangladesh. Am. Eurasian J Sci Res. 2007;2:121-125.
6. Moktaduzzaman M, Rahman SMM. Regeneration of Stevia rebaudiana and analysis of somaclonal variation by RAPD. Biotech. 2009;8:449-455.
7. Anbazhagan M, Kalpana M, Rajendran R, Natarajan V, Dhanavel D. In vitro production of Stevia rebaudiana Bertoni. Emir J Food Agric. 2010;22:216-222.
8. Alhady MRAA. Micropropagation of Stevia rebaudiana Bertoni: A new sweetening crop in Egypt. Glob. J Biotechnol Biochem. 2011;6:178-182.
9. Singh N, Yadav K, Kumari S. Metabolic changes during differentiation in callus cultures of Stevia rebaudiana (Bertoni). J Phytol. 2011;3:63-67.
10. Hassanen SA, AKhalili RM. Biotechnological studies for improving of stevia (Stevia rebaudiana Bertoni) In vitro plantlets. Middle-East J Sci Res. 2013;14:93-106.
11. Jitendra M, Monika S, Ratan SD, Priyank G, Priyank S, Jayraykiran D. Micropropagation of an antidiabetic plant- Stevia rebaudiana of south-east rajasthan, india, ISCA J biolo sci. 2012;1:37-42.
12. Zayova E, Stancheve I, Mgeneva M, Petrova M, Dimitrova L. Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins, Turk Boil. 2013;37:106-113.
13. Verma S, Kuldeep Y, Singh N, Optimization of the protocols for surface sterilization, regeneration and acclimatizeation of Stevia rebaudiana Bertoni, American-Eurasian. J Agric Environ Sci. 2011;11:222-227.
14. Mehrafarin A, Etminan AR, Delkhosh B, Golrokhan M. Assessment of Stevioside Content, Shoot Proliferation, and Root Induction of Stevia rebaudiana Bertoni under In vitro Conditions. J Med Plants. 2016;15:98-110.
15. Singh M, Saharan V, Dayma J, Rajpurohit D, Sen Y, Sharma A. In vitro Propagation of Stevia rebaudiana (Bertoni): An Overview. Int J Curr Microbiol App Sci. 2017;6:1010-1022.
16. Ferreira CM, Handro W. Production, maintenance and plant regeneration from cell suspension cultures of Stevia rebaudiana (Bert.) Bertoni. Plant Cell Rep. 1988;7:123-126.
17. Razak UNAA, Ong CB, Yu TS, Lau LK. In vitro Micropropagation of Stevia rebaudiana Bertoni in Malaysia. Braz Arch Biol Technol. 2014;57:23-28.
18. Jain P, Kachhwaha S, Kothari SL. Biotehcnology and metabolic engineering of Stevia rebaudiana (Bert.) Bertoni: Perspective and Possibilities. Int J Life Sc Bt Pharm. Res. 2014;3:1-23.
19. Ladygin VG, Bondarev NI, Semenova GA, Smolov AA, Reshetnyak OV, Nosov AM. Chloroplast ultrastructure, photosynthetic apparatus activities and production of steviol glycosides in Stevia rebaudiana in vivo and in vitro. Biol Plant. 2008;52:9-16.
20. Woelwer-Rieck U, Lankes C, Wawrzun A, Wu¨st M. Improved HPLC method for the evaluation of the major Steviol glycosides in leaves of Stevia rebaudiana. Eurasian J Food Res Technol. 2010; 231: 581–588.
21. Kumari M,  Chandra S. Phytochemical studies and estimation of major steviol glycosides in varied parts of Stevia rebaudiana. Int J Pharm Pharm Sci. 2015;7:62-65.
22. Singh P, Dwivedi P. Two-stage culture procedure using thidiazuron for efficient micropropagation of Stevia rebaudiana, an antidiabetic medicinal herb. 3 Biotech. 2014;4:431-437.
23. Tamura Y, Nakamura S, Fukui H, Tabata M. Comparison of Stevia plants grown from seeds, cuttings and stem tip cultures for growth and sweet diterpene glycosides. Plant Cell Rep. 1984;3:180-182.
24. Komatsu K, Nozaki W, Takemura M. Nakaminami M. Production of natural sweetener. ChemAbstr. 1976; 84, 169.
25. Karim MdZ, Uesugi D, Nakayama N, Hossain MM, Ishihara K, Hamada H. Identification of Stevioside Using Tissue Culture-Derived Stevia (Stevia rebaudiana) Leaves. Biochem. 2015;8:33-37.
26. Fadel D, Kintzios S, Economou AE, Moschopoulou G, Constantinidou HIA. Effect of different strength of medium on organogenesis, phenolic accumulation and antioxidant activity of spearmint (Mentha spicata L.). The Open Hort. J. 2010;3:5- 31.
27. Malekirad M, Nasr N, Kazemitabar SK. Study on some Stevia (Stevia rebaudiana Bertoni) metabolites in the invitro and invivo conditions using GC- MS. J Med Plants Biotechnol. 2016;2:41- 54.
28. Tahmasi S, GaroosiGh A, Ahmadi J, Farjaminezhad R. Effect of salicylic acid on stevioside and rebaudioside a production and transcription of biosynthetic genes in in vitro culture of Stevia rebaudiana. Iran J Genet Plant Breed. 2017;6:1-8.
29. Rafiq M, Muhamamd MU, Mangrio S, Naqvi HA, Qarshi IA. In vitro clonal propagation and biochemical analysis of filed established Stevia rebaudiana Bertoni Pak J Bot. 2007;39:2467-2474.
Journal of Medicinal plants and By-product
Volume 9, Issue 1
April 2020
Pages 1-8

Files

Share

How to cite

Statistics