Effects of Seed Priming on Germination Characteristics of Achillea millefolium Seeds under Different Ageing Treatment

Document Type : Research Paper

Authors

1 Natural Resources Gene Bank, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization, Tehran, Iran

2 Rangeland Research Division, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization, Tehran, Iran

Abstract

The genus Achillea L. (Compositae) widely use as medicinal plant possessing several pharmacological effects due to presence of active compounds. In order to study of seed priming effects on seedling growth of yarrow, a factorial experiment, based on randomized complete design with three replications was conducted under greenhouse conditions in Research Institute of Forests and Rangeland (RIFR) for one year. Experimental factors were a) three Achillea millefolium L. accessions originated from Kordestan (14303), Semnan (21602) and Gilan (27028), provinces, b) five conservation methods including: medium-term storage (Active Collection at 4 °C,10 or 15 years), long-term storage (Base Collection at -18 °C,10 or 15 years), regenerated seeds in open storage 22 °C for2 years (Control) and aged seed under accelerated ageing (40 °C,98% of relative humidity for 48 and 72h) and c) priming treatments including: non- priming (control), osmo-priming (PEG-0.3Mpa), hormonal priming (gibberellic acid at250 and 500ppm).Data collected for seed emergence percent, root and shoot length, seedling length, vigor index, seedling weight and three peroxidase, catalase and superoxide dismutase (SOD) enzymatic activities. Result of analysis of variance showed significant effects of all factors and their interactions for all of seedling traits and enzymatic activities except for SOD (P<0.01).Results also suggested significant differentiation among three accessions of A. millefolium for all germination traits and enzymes activities. Results showed that responses of accessions to aging and priming treatments were different. The data demonstrated that catalase is a key enzyme for seed repair against ageing ROS-induced damage during priming treatment.

Keywords

References

1. FAO. Draft genebank standards for plant genetic resources for food and agriculture. 2013. http://www.fao.org/agriculture/crops/corethemes/theme/seeds-pgr/conservation/gbs/en/.
2. Royal Botanic Gardens Kew Seed Information Database (SID). 2008; Version 7.1. http://data.kew.org/sid/
3. Priestley D.A, Seed Aging. Implications of Seed Storage and Persistence in the Soil, Cornell University Press, Ithaca, NY. 1986.
4. Kibinza S, Vinel D, Côme D, Bailly C, Corbineau F. Sunflower seed dete- rioration as related to moisture contentduring ageing, energy metabolism and active oxygen species scavenging, J Physiol Plant. 2006;128:496-506.
5. Harman D. Aging: a theory based on free radical and radiation chemistry. J Gerontol. 1956;11:298-300.
6. Harman D, Free radical theory of aging: history. In free radicals and aging, Birkhäuser, Basel. 1992;1-10.
7. Bailly C, Benamar A, Corbineau F, Côme D. Changes in malondialdehyde con- tent and insuperoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. J Physiol Plant. 1996; 97:104-110.
8. Kranner I, Birtic S, Anderson KM, Prichard HW. Glutathione half-cell reduc- tion potential: a universal stress marker and modulator of programmed cell death, Free Radic Biol Med. 2006;40:2155-2165.
9. Bailly C, Benamar A, Corbineau C, Côme D. Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds. J Plant Physiol. 1998;104:646-652.
10. Goel A, Goel AK, Sheoran IS. Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypiumhirsutum L.) seeds, J. Plant Physiol. 2003;160:1093-1100.
11. Bienert GP, Schjoerringa JK, Jahn TP. Membrane transport of hydrogen per-oxide, Biomembranes. 2006;1758:994-1003.
12. Orr WC, Sohal RS. Extension of life-span by overexpression of superoxide dis- mutase and catalase in Drosophila melanogaster. Sci. 1994;263:1128-1130.
13. Sun J, Tower FLP. Recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. J Mol Cell Biol. 1999;19:216-228.
14. Sampayo JN, Gill MS, Lithgow GJ. Oxidative stress and aging–the use of superoxide dismutase/catalase mimetics to extend lifespan. Biochem Soc Trans. 2003;31:1305-1307.
15. Cutler RG, Oxidative stress and aging:catalase is a longevity determinant enzyme Rejuvenation Res. 2005;8:138-140.
16. Gallego SM, Benavídes MP, Tomaro ML. Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress. Plant Sci. 1996;121:151-159.
17. Park SJ, Huang Y, Ayoubi P. Identification of expression profiles of sorghum genes in response to greenbug phloem-feeding using cDNA subtraction and microarray analysis. Planta. 2006;223:932-947.
18. McDonald MB. Seed Priming. In: Seed Technology and its Biological Basis (Eds. Black, M., Bewley, J.D.) Sheffield Academic Press, Sheffield, UK. 2000;287–325.
19. Butler LH, Hay FR, Ellis RH, Smith RD, Murray TB. Priming and re-drying improve the survival of mature seeds of Digitalis purpurea during storage. Ann Bot. 2009;103:1261-1270.
20. Ehsanfar S, Modarres-Sanavy SA, Tavakkol-Afshari R. Effects of osmopriming on seed germination of canola (Brassica napus L.) under salinity stress, Commun. Agric. Appl. Biol. Sci. 2006;71:155-159.
21. Lanteri S, Kraak HL, De Vos CHR, Bino RJ. Effects of osmotic preconditioning on nuclear replication activity in seeds of pepper (Capsicum annuum). J Plant Physiol. 1993;89:433-440.
22. Soeda Y, Konings MCJM, Vorst O, Van Houwelingen AMML, Stoopen GM, Maliepaard CA, Kodde J, Bino RJ, Groot SPC, van der Geest AHM. Gene expression programs during Brassica oleracea seed maturation, osmopriming, and germination are indicators of progression of the germination process and the stress tolerance level. J Plant Physiol. 2005;137:354–368.
23. Burgass RW, Powell AA. Evidence for repair processes in the invigoration of seeds by hydration. Ann Bot. 1984;53:753-757.
24. Sivritepe HO, Dourado AM. The effect of priming treatments on the viability and accumulation of chromosomal damage in aged pea seeds. Ann Bot. 1995;75:165-171.
 25. Gallardo K., Job C., Groot S.P.C, Puype M, Demol H, Vandekerckhove J, Job D, Proteomic analysis of Arabidopsis seed germination and priming. J Plant Physiol. 20012;7126:835-848.
26. Jeng TL, Sung JM. Hydration effect on lipid peroxidation and peroxide scav-enging enzyme activity of artificially aged peanut seed. Seed Sci. Technol. 1994;22:531-539.
27. Aburjai T, Hudaib M. Antiplatelet, antibacterial and antifungal activities of Achillea falcata L. extracts and evaluation of volatile oil composition. Pharmacog Mag. 2006;2:191-8.
28. Haidara k, Zamir L, Shi QW, Batist G. The flavonoid Casticin has multiple mechanisms of tumor cytotoxicity action. Cencer Letters. 2006;242:180-190.
29. Afsharpuor S, Asgary S. Volatile constituents of Achillea millefolium subsp. millefolium from Iran. J Flavour fragrance.1996;11:265-267.
30. David R, Zbigniew A. Aqueous extract of Achillea millefolium L. (Asteraceae) inflorescences suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 murine macrophages. J Med Plants Res. 2010;4:225-34.
31. Abdual-baki AA, Anderson JD. Relationship between decarboxylation of glutamic acid and vigor in soybean seed. Crop Sci. 1973;13:222-226.
32. ISTA, Handbook on Seedling Evaluation. Int Seed Testing Assoc. Zurich. 2008;519.
33. Lekh R, Khairwal IS. Evaluation of pearl millet hybrids and their parents for germ inability and field` emergence. Indian J Plant Physiol. 1993;2:125
34. Abdual-baki, AA, Anderson, JD. Relationship between decarboxylation of glutamic acid and vigor in soybean seed. Crop Sci. 1973;13:222-226.
35. Shannon LM, Kay E, Law JY.  Peroxidase isoenzyme from horse radish roots: isolation and physical properties. J Biol Chem. 1966;241:2166-2172.
36. Aebi H.  Catalase in vitro. Methods Enzymol. 1984;105:121-126
37. Giannopolitis CN, Ries SK. Superoxide dismutase. I. Occurrence in higher plants. J Plant Physiol. 1977;59:309-314.
38. Elstner EF, Youngman R, Obwald W. Superoxide dismutase. In Bergmeyer J, Grabl BM (eds) Methods of Enzymatic Analysis vol. III. Enzymes oxidoreductases, 3rd ed. Weinheim: Verlag-Chemie. 1995:293-302. 
39. Rincker CM. Germination of forage crop seeds after 20 years of subfreezing storage. Crop Sci. 1983;23:229-231.
40. Farooq M, Basra SMA, Tabassum R, Afzal I. Enhancing the performance of direct seeded fine rice by seed priming. Plant Prod. Sci. 2006;9:446-456.
41. Alivand, R, Tavakol Afshari R, Sharif Zadeh F. Effects of gibberellins, salicylic acid, and Ascorbic acid on improvement of germination characteristics on deteriorated seeds of Brassica napus. Iranian J Agric Sci. 2013;43:561-571.
42. SajjadiJaghoroghi SS, Alizadeh MA, Kalagari M. Effect of Osmopriming, Hydropriming and Pre-chilling on Seed Emergence Enhancement and Seedling Vigor of four Medicinal Species of Anthemis under Greenhouse Conditions, Bulletin UASVM Horticulture. 2014;71:74-84.
43. Bailly C. Active oxygen species and antioxidants in seed biology. Seed Sci Res. 2004;14:93-107.
44. Goel A, Goel AK, Sheoran IS. Changes in oxidative stress enzymes during artificial aging in cotton (Gossypium hirsutum L.) seeds. J Plant Physiol. 2002; 160:1093-1100.
45. McDonald MB. Orthodox seed deterioration and its repair, In: Handbook of Seed Physiology: Applications to Agriculture, Benech-Arnold, R. L. and Sanchez (Eds.) R.A. Food Products Press, New York, 2004:273-304.
46. Siadat SA, Moosavi A, Sharafizadeh M. Effect of seed priming on antioxidant activity and germination characteristics of Maize seeds under different aging treatments. Res J Seed Sci. 2012;5:51-62.
47. Alizadeh MA. Evaluation of percentage of germination, total speed of germination and vigor index of 17 medicinal plants species to ageing test. Proceeding of National Congress in Sustainable Development of Medicinal Plants, (NCSDMP), 27-29 July (2005) Mashhad. 2005;171-172.
48. Jan-Mohammadi M, Fallahnezhad F, Golsha M, Mohammadi H Controlled aging for storability assessment and predicting seedling early growth of canola cultivars (Brassica napus L.). ARPNJ.Agric Biol Sci. 2008;3:22-26.
49. Ghassemi-Golezani K, Khomari S, DaliliB Hosseinzadeh-Mahootchy B, Chadordooz-Jedi A. Effect of seed aging on field performance of winter oil seed rape. J Food AgricEnvir. 2010;8:175-178.
50. Bhattacharjee A, Kanp UK, Chakrabarti D, Pati CK. Technique for storage longevity of mung bean and sunflower seeds using sodium dikegulac and Eucalyptus oil. Bangla J Bot. 2006;3:55-61.
51. Saha RR, Sultana W. Influence of seed aging on growth and yield of soybean. Bangla J Bot. 2008;37:21-26.
52. Moradi A, Younesi O. Effects of Osmo- and Hydro-priming on Seed Parameters of Grain Sorghum (Sorghum bicolor L.). Australian J Basic Appl Sci. 2009;3:1696-1700.
53. Akhter FN, Kabir G, Mannan MA, Shaheen NN. Aging effect of wheat and barley seeds upon germination mitotic index and chromosomal damage. J Islam Acad Sci. 1992;5:44-48.
54. Nautiyal AR, Thapliyal AP, Purohit AN. Seed viability. IV. Protein changes: Accompanying loss of viability in Shorearobusta. Seed Sci Technol. 1985;13:83-86.
55. Abdalla FH, Roberts EH. Effects of temperature, moisture and oxygen on the induction of chromosome damage in seeds of barley, broad beans and peas during storage. Ann Bot (N.S.). 1968; 32:119-136.
56. Rasoolzadeh L, Salehi Shanjani P, Madani H. Effect of Priming on  Germination and Enzyme Activity of Achillea vermicularis Seeds after Naturally and Accelerated Aging. J Med Plants By-products. 2017;1:11-16.
57. Kibinza S, Bazina J, Bailly C, Farrant JM, Corbineaua O, Bouteaua H. Catalase is a key enzyme in seed recovery from aging during priming. Plant Sci. 2011;181:309-315.
58. Kroemer G, Petit P, Zamzami N, Vayssiere JL, Mignotte B. The biochemistry of programmed cell death. FASEB J. 1995;9:1277-1287.
59. Able AJ, Guest DI, Sutherland MW. Hydrogen peroxide yields during the incompatible interaction of tobacco suspension cells inoculated with Phytoph-thoranicotianae. J Plant Physiol. 2000;124:899-910.
 
Journal of Medicinal plants and By-product
Volume 9, Issue 1
April 2020
Pages 79-89

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