Eradication of Biofilm Formation by Crocus sativus Alcoholic Extract in Streptococcus mutans Clinical Isolates

Document Type : Research Paper

Authors

1 Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

2 Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam, Iran

3 Department of Pediatric Dentistry, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran

Abstract

Despite major advances in oral health in the past decades, tooth decay is one of the most common preventable diseases in the worldwide. Nowadays, Streptococcus mutans is discussed as one of the most important challenges in tooth decay. Also, medicinal herbs can be considered as an effective weapon against infectious diseases. The purpose of the current study was to investigate the biofilm formation in S. mutans clinical isolates and to evaluate the anti-biofilm properties of Crocus sativus against S. mutans clinical isolates. In this study, thirty dental plaque samples were collected. Then, identification of samples was performed by standard methods. Biofilm formation was evaluated on S. mutans clinical isolates. C. sativus alcoholic extract was applied as an anti-biofilm formation in this isolates. Our results demonstrated that a significant number of S. mutans samples were identified as biofilm producers. Then, C. sativus in 60 mg/ml, 30 mg/ml and 8 mg/ml were able to eradicated strong, moderate and week biofilm formation in thisisolates, respectively. In addition, more extensive studies and in vivo research are needed to confirm the results of this study.


Keywords

References

1. Peres MA, Macpherson LM, Weyant RJ, Daly B, Venturelli R, Mathur MR, et al. Oral diseases: a global public health challenge. The Lancet. 2019;394:249-60.
2. Warrilow L, Dave R, McDonald S. Oral health promotion: Right person, right time prevention. J Health Visit. 2019;7:394-8.
3. Abranches J, Zeng L, Kajfasz JK, Palmer S, Chakraborty B, Wen Z, et al. Biology of oral streptococci. Gram‐Positive Pathogens. 2019:426-34.
4. Waggoner WF, Nelson T. Restorative dentistry for the primary dentition.  Pediatric Dentistry: Elsevier; 2019. p. 304-28. e3.
5. Zeng L, Burne RA. Essential roles of the sppRA fructose-phosphate phosphohydrolase operon in carbohydrate metabolism and virulence expression by Streptococcus mutans. J bacteriology. 2019;201:e00586-18.
6. Wen ZT, Scott‐Anne K, Liao S, De A, Luo M, Kovacs C, et al. Deficiency of BrpA in Streptococcus mutans reduces virulence in rat caries model. Molecular oral microbiology. 2018;33:353-63.
7. Bowen WH, Burne RA, Wu H, Koo H. Oral biofilms: pathogens, matrix, and polymicrobial interactions in microenvironments. Trends in microbiology. 2018;26:229-42.
8. Nilsson M, Jakobsen TH, Givskov M, Twetman S, Tolker-Nielsen T. Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms. Microbiology. 2019;165:334-42.
9. Jalili M, Amraei M, Sadeghifard N, Ghafourian S. Evaluation of Biofilm Formation and Anti-biofilm Properties of and in Clinical Isolates. The Open Micro J. 2019;13.
10. Bacha K, Tariku Y, Gebreyesus F, Weiland-Bräuer N, et al. Antimicrobial and anti-Quorum Sensing activities of selected medicinal plants of Ethiopia: Implication for development of potent antimicrobial agents. BMC microbiology. 2016;16:139.
11. Panda SK, Das R, Leyssen P, Neyts J, Luyten W. Assessing medicinal plants traditionally used in the Chirang Reserve Forest, Northeast India for antimicrobial activity. J Ethnophar. 2018;225:220-33.
12. Bragança I, Lemos PC, Barros P, Delerue-Matos C, Domingues VF. Phytotoxicity of pyrethroid pesticides and its metabolite towards Cucumis sativus. Sci Total Environ. 2018;619:685-91.
13. Adeogun OO, Maroyi A, Afolayan AJ. Effects of Leaf Extracts of Ocimum gratissimum L. on quality of fresh cut Cucumis sativus L. Asian J Plant Pathology. 2017;11:174-84.
14. Mowat E, Butcher J, Williams C, Ramage G. Aspergillus fumigatus biofilms are refractory to antifungal challenge. 2007.
15. Yadav K, Prakash S. Dental caries: A microbiological approach. J Clin Infect Dis Pract. 2017;2:1-15.
16. Pérez-Sánchez T, Mora-Sánchez B, Balcázar JL. Biological approaches for disease control in aquaculture: advantages, limitations and challenges. Trends in microbiology. 2018;26:896-903.
17. Khelissa SO, Abdallah M, Jama C, Faille C, Chihib N-E. Bacterial contamination and biofilm formation on abiotic surfaces and strategies to overcome their persistence. J Mater Environ Sci. 2017;8:3326-46.
18. Das T, Manos J. Pseudomonas aeruginosa Extracellular Secreted Molecules Have a Dominant Role in Biofilm Development and Bacterial Virulence in Cystic Fibrosis Lung Infections. Progress in Understanding Cystic Fibrosis. 2017;101.
19. Deka P, Passari AK, Singh BP. Actinobacteria as a potential natural source to produce antibiofilm compounds: An overview.  New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Biofilms: Elsevier; 2020. p. 91-9.
20. Takahashi H, Nadres ET, Kuroda K. Cationic amphiphilic polymers with antimicrobial activity for oral care applications: Eradication of S. mutans biofilm. Biomacromolecules. 2017;18:257-65.
21. Avneet G, Pal SM, Siddhraj SS. A review on herbal Ayurvedic medicinal plants and its association with memory functions. 2018.
22. Khalil N, Ashour M, Fikry S, Singab AN, Salama O. Chemical composition and antimicrobial activity of the essential oils of selected Apiaceous fruits. Future J Pharmaceutical Sci. 2018;4:88-92.
Journal of Medicinal plants and By-product
Volume 10, Special
April 2021
Pages 39-42

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