Eradication of Biofilm Formation by Crocus sativus Alcoholic Extract in Streptococcus mutans Clinical Isolates

Document Type : Research Paper


1 Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

2 Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam, Iran

3 Department of Pediatric Dentistry, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran


Despite major advances in oral health in the past decades, tooth decay is one of the most common preventable diseases in the worldwide. Nowadays, Streptococcus mutans is discussed as one of the most important challenges in tooth decay. Also, medicinal herbs can be considered as an effective weapon against infectious diseases. The purpose of the current study was to investigate the biofilm formation in S. mutans clinical isolates and to evaluate the anti-biofilm properties of Crocus sativus against S. mutans clinical isolates. In this study, thirty dental plaque samples were collected. Then, identification of samples was performed by standard methods. Biofilm formation was evaluated on S. mutans clinical isolates. C. sativus alcoholic extract was applied as an anti-biofilm formation in this isolates. Our results demonstrated that a significant number of S. mutans samples were identified as biofilm producers. Then, C. sativus in 60 mg/ml, 30 mg/ml and 8 mg/ml were able to eradicated strong, moderate and week biofilm formation in thisisolates, respectively. In addition, more extensive studies and in vivo research are needed to confirm the results of this study.


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