Evaluation of Camelina sativa Doubled Haploid Lines for the Response to Water-deficit Stress

Document Type : Research Paper

Authors

Department of Agronomy and Plant Breeding, Faculty of Agriculture, Razi University, Kermanshah, Iran

Abstract

Camelina is low input and re-emergent medicinal and oilseed crop that it needs a lot of research to make the most of its genetic potential. This study aimed to evaluate the water deficit tolerance of six genotypes (Soheil cultivar and DH083, DH095, DH111, DH116, and DH133 doubled haploid lines)of camelina for some traits in the greenhouse and laboratory conditions. In the greenhouse condition, the response of camelina genotypes to irrigating regime (once every 4, 7 and 10 days) was investigated for some agro-morphological traits. This experiment was done in a randomized complete block design. Analysis of variance showed that the studied genotypes, as well as drought stress levels, were significantly different in terms of plant height, root length, root weight, grain yield, biological yield and harvest index. Mean comparison results also showed that the Soheil cultivar had the highest grain yield (3.03 g/m2). At the laboratory section and the cotyledon culture and callus induction experiment, six lines were evaluated by four levels of PEG 6000 (0, 10, 20 and 30%) for water-deficit stress in a randomized completely design. The callus growth rate, relative callus growth rate and relative callus water content under stress conditions were also studied. There was a significant difference between studied genotypes in studied traits under stress conditions and between different stress levels. In this section, the Soheil cultivar was identified as high tolerant cultivar among other genotypes due to its relative water content (8.3%) and higher callus growth rate (4.2%). So the cultivar Soheil is considered as the least susceptible to dehydration in terms of greenhouse studies and callus induction.

Keywords

References

1. Kahrizi D, Rostami-Ahmadvandi H, Akbarabadi A. Feasibility Cultivation of Camelina (Camelina sativa) as Medicinal-Oil Plant in Rainfed Conditions in Kermanshah-Iran's First Report. J Med Plants By-product. 2015;4:215.b
2. Khalili M, Naghavi MR Aboughadareh  AP, Talebzadeh S J. Evaluating of drought stress tolerance based on selection indices in spring canola cultivars (Brassica napus L.). J Agric Sci. 2012;4:78.
3. Ahmed IM, Cao F, Han Y, Nadira UA, Zhang G, Wu F. Differential changes in grain ultrastructure, amylase, protein and amino acid profiles between Tibetan wild and cultivated barleys under drought and salinity alone and combined stress. Food chem. 2013;141:2743.
4. Laxa M, Liebthal M, Telman W, Chibani K, Dietz KJ. The role of the plant antioxidant system in drought tolerance. Antioxidants. 2019;8:94.
5. Falaknaz  M , Alaami  A, Mehrabi  A, Sabouri A, Kahrizi D, Karimi  N. Cellular and physiological responses to drought stress in Aegilops tauschii genotypes. Cell Mol Biol (Noisy-le-Grand, France). 2019;65:84.
6. Swati Z, Muhammad A I, Hayataj F.. In situ and In vitro studies in wheat (Triticum aestivum) genotypes for drought tolerance. Treatment of plant breeding and genetic faculty of crop production Science. 2003;197 pp.
7. Satyavathi V, Jauhar P, Elias E, Rao M. Effects of growth regulators on in vitro plant regeneration in durum wheat. Crop Sci. 2004;44:1839.
8. Karimi S, Yadollahi A, Arzani K. Responses of almond genotypes to osmotic stress induced in vitro. J Nuts. 2013;4:1-7.
9. Rasti Sani M, Ganjeali A, Lahouti M, Mousavi Kouhi SM. Morphological and physiological responses of two common bean cultivars to drought stress. J Plant Process Func. 2018;6:37.
10. Bortolheiro FP, Silva MA. Physiological response and productivity of safflower lines under water deficit and rehydration. Anais da Academia Brasileira de Ciências. 2017;89:3051.
11. Javed F, Ikram S. Effect of sucrose induced osmotic stress on callus growth and biochemical aspects of two wheat genotypes. Pakistan J Botany. 2008;40:1487.
12. Zebarjadi A, Ghasempour H, Soheilikhah Z. Effects of drought stress on biochemical and physiological parameters in callus cultures of Carthamus tinctorius varieties. Acta Agronomica Hungarica. 2010;58:395.
13. Al-Khayri J, Al-Bahrany A. Growth, water content, and proline accumulation in drought-stressed callus of date palm. Biologia Plantarum. 2004;48:105-108.
14.Kakaei M, Mansouri M, Abdollahi M, Moradi F. Effect of NaCl and PEG induced osmotic stress on callus growth parameters of two Safflower (Carthamus tinctorius L.) cultivars. Inter J Agric Crop Sci. 2013;6:127.
15.   Jouyban A, Give HS, Noryan M. Relationship between agronomic and morphological traits in barley varieties under drought stress condition. Int Res J Appl Bas. Sci. 2015;9:1507.
16. Tabassam MA, Hussain M, Sami A, Shabbir I, Bhutta MA, Mubusher M, Ahmad S. Impact of drought on the growth and yield of wheat. Sci Agric. 2014;7:11-8.
Journal of Medicinal plants and By-Products
Volume 9, Issue 2
September 2020
Pages 193-199

Files

Share

How to cite

Statistics