Extraction of Thymol Compound from Thymus vulgaris L. Oil

Document Type : Research Paper

Authors

Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Essential oils consist of many chemical compounds with different structures and functional properties, which might be employed as an ingredient in many foods, drug formulations, and products. In this research, leaves and aerial part of cultivated Thymus vulgaris L. was isolated by hydrodistillation. The chemical compositions of the essential oil were identified by the application of GC/MS. Then thymol, as the main ingredient, was purified by hot water or microwave methods. Subsequently, the yield and degree of purity of extracted thymol were determined. The results indicated the extraction efficiency of leaves essential oil (1.16 %) was more than aerial parts (0.62 %) and thymol the main ingredient in both essential oils includes 59.47 and 53.63 %, respectively. The highest yield of thymol extracted (91.27 %) was obtained from leaves with a purity of 95.27% by microwave extraction method. Extracted thymol might be recommended for use in formulation of the food and drug supplements due to numerous functional and pharmacological activities.

Keywords

References

1. Anzlovar S, Baricevic D, Avgustin JA, Koce JD. Essential Oil of Common Thyme as a Natural Antimicrobial Food Additive. Food Technol Biotech. 2014;52:263-268.
2. Goldansaz SM, Rezaee MB, Jaimand K, Mirhoseini A. Study of Essential Oils Compositions of Three Species of Medicinal Plants from ShirKooh Mountain (Up village) on Yazd Province. J Med Plant and By-prod. 2014;2:187-191.
3. Mosleh Arani A, Naderi M, Goldansaz SM. Effect of Harvesting Time on Essential Oil Content and Composition of Thymbra spicata. J Med Plant By-prod. 2015;1:51-55.
4. Amiri F, Gholipouri A, Kheirkhah M, Mirjalili M.H. Study on Ethnobotany and the Effect of Ecological Factor on the Yield of Essential Oil of Ziziphora clinopodioides Lam. (Case Study: Yazd Province). J Med Plant By-prod. 2019;8:105-206.
5. Goldansaz SM, Jafarian Jeloudar Z, Safaeian R, Sonboli A. Comparison of the chemical constitutions, antibacterial, anti-Candida, and antioxidant activity of Nepeta asterotricha Rech. f. essential oil. America J Essen Oils Nat Prod. 2019;7:15-22.
6. Braga PC. Thymol: antibacterial, antifungal and antioxidant activities. Giorn It Ost Gin. 2005;27:7-8.
7. Bermejo DV, Angelov LA, Vicente G, Stateva RP, García-Risco MR, Reglero G, Ibáñez E, Fornari T. Extraction of thymol from different varieties of thyme plants using green solvents. J Sci Food Agric. 2015;95:2901-2907
8. Salehi B, Mishra AP, Shukla L, Sharifi‐Rad M, Contreras M, Segura‐Carretero A, Fathi H, Nasri Nasrabadi N, Kobarfard F, Sharifi‐Rad J. Thymol, thyme, and other plant sources: Health andpotential uses. Phytotherapy Res. 2018;32:1688-1706.
9. Borgarello AV, Mezza GN, Pramparo MC, Gayol MF. Thymol enrichment from oregano essential oil by molecular distillation. Separation Purification Technol. 2015;153:60-66.
10. Gharachorloo M, Honarvar M, Mardani SH. Chemical compositions and antioxidant activity of Heracleum persicum essential oil. Brazilian J Pharma Sci. 2017;53:e00260.
11. Phillips M, Gibbs HD. A synthesis of thymol from p-Cymene. J Indust Engineering Chem. 1920;12:733-34.
12. Koshk Abdi H. Pharmaceutical Chemistry of Steroid and Terpene Compounds. Tehran University Press. 1984;121-123.
13. Porte A, Godoy RLO. Chemical composition of Thymus vulgaris L. (thyme) essential oilfrom the Rio de Janeiro State (Brazil). J Serbian Chem Soci. 2008;73:307-310.
14. Lemrhari A, Zouhair R, El Kahkahi R, Elidrissi M, Amchrouk A, Elhourri M. Chemical composition and differentiation of essential oils of Morocco’s different varieties of thyme. Global J Pure Applied Chem Res. 2016;4:40-49.
15. Satyal P, Murray BL, McFeeters RL and Setzer WN. Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations. Foods. 2016;5:1-12.
Journal of Medicinal plants and By-product
Volume 10, Issue 1
April 2021
Pages 81-84

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