Extraction and Phytochemical Analysis of Anthemis susiana Nábělek Using Headspace-solid Phase (HS-SPME) and Gas Chromatography–mass Spectrometry (GC-MS)

Document Type : Research Paper

Authors

1 Department of Internal Medicine, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran

2 Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran

3 Department of Nephrology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran

Abstract

Chamomile has several variants, such as Anthemis Susiana Nábělek, Matricaria chamomilla L., Chamomilla recutita (L.) Rauschert, Matricaria suaveolens Koch and etc. Medicinal herbs, extracts and essential oils are of particular importance in the pharmaceutical, cosmetic and food industries. The phytoanalysis of these compounds is of particular importance. In April 2019, Anthemis Susiana Nábělek samples were collected from the Thermal Springs area of ​​Dehloran, south of Ilam province, western Iran. The plant was dried and then pulverized. The essential oil was extracted from the plant using HS-SPME and analyzed using GC-MS. GC-MS results showed that Anthemis susiana Nábělek contains 63 chemical compounds. The main chemical compounds included alpha-bisabolol oxide A (19.07%), alpha-pinene (15.50%), beta-bisabolene (12.56%), spathulenol (9.23%), beta-farnesen (8.95%), alpha-bisabolol (4.72%), caryophyllene oxide (4.46%), trans-farnesol (3.75%), and dl-limonene (3.47%).

Keywords


1. Singh O, Khanam Z, Misra N, Kumar Srivastava M. Chamomile (Matricaria chamomilla L.): An overview. Pharmacogn Rev. 2011;5:82-95.
2. Ghafoor A. The genus Anthemis L. (Compositae-anthemideae) in Arabia peninsula: a taxonomic study. Pak J Bot. 2010;42:79-98.
3. Javidnia K, Miri R, Kamalinejad M, Sarkarzadeh H, Jamalian A. Chemical composition of the essential oils of Anthemis altissima L. grown in Iran. Flavour Frag J. 2004;19:213-216.
4. Kivcak B, Mert T, Saglam H, Ozturk T, Kurkcuoglu M, Baser KHC. Chemical composition and antimicrobial activity of the essential oil of Anthemis wiedemanniana from Turkey. Chem Nat Comp. 2007;43:47-51.
5. Rezaee MB, Jaimand K, Assareh MH. Chemical constituents of the leaf and flower oils from Anthemis altissima L. var. altissima from Iran. J Ess Oil Res. 2006;18:152-153.
6. Al-Humaidi J. Chemical composition of the essential oils for Anthemis melampodina from North Saudi Arabia. Oriental J Chem. 2015;31:2265-2270.
7. Pavlovic M, Kovacevic N, Tzakou O, Couladis M. Components of cyclohexane extract of Anthemis triumfetti. Chem Nat Compd. 2007;43:512-514.
8. Hajdú Z, Zupkó I, Réthy B, Forgo P, Hohmann J. Bioactivity-guided isolation of cytotoxic sesquiterpenes and flavonoids from Anthemis ruthenica. Planta Med. 2010;76:94-96.
9. Srivastava JK, Gupta S. Antiproliferative and apoptotic effects of chamomile extract in various human cancer cells. J Agric Food Chem. 2007;55:9470-8.
10. Cemek M, Kaða S, Simþek N, Büyükokuroðlu ME, Konuk M. Antihyperglycemic and antioxidative potential of Matricaria chamomilla L. in streptozotocin-induced diabetic rats. Nat Med. 2008;62:284-93.
11. Mozaffarian VA. Flora of Ilam Province. Contemporary Culture Publication, Tehran. 2008;114.
12. Moayeri A, Azimi M, Karimi E, Aidy A, Abbasi N. Attenuation of Morphine Withdrawal Syndrome by Prosopis Farcta Extract and Its Bioactive Component Luteolin in Comparison with Clonidine in Rats. Med Sci Monitor Basic Res. 2018;24:151-158.
13. Bahmani M, Taherikalani M, Khaksarian M, Rafieian-Kopaei M, Ashrafi B, Nazer M, Soroush S. Abbasi N, Rashidipour M. The synergistic effect of hydroalcoholic extracts of Origanum vulgare, Hypericum perforatum and their active components carvacrol and hypericin against Staphylococcus aureus. Future Science OA. 2019;5: Article number FSO371.
14. Abbasi N, Khosravi A, Aidy A, Shafiei M. Biphasic response to luteolin in MG-63 osteoblast-like cells under high glucose-induced oxidative stress. Iranian J Med Sci. 2016;41:118-125.
15. Karimi E, Abbasi S, Abbasi N. Thymol polymeric nanoparticle synthesis and its effects on the toxicity of high glucose on OEC cells: Involvement of growth factors and integrin-linked kinase. Drug Design, Developm Therap. 2019;13:2513-2532.
16. Lord H, Pawliszyn J. Evolution of solid-phase microextraction technology. J Chromatography A. 2000;885:153-193.
17. Bahmani M, Taherikalani M, Khaksarian M, Soroush S, Ashrafi B, Heydari R. Phytochemical Profiles and Antibacterial Activities of Hydroalcoholic Extracts of Origanum vulgare and Hypericum perforatum and Carvacrol and Hypericin as a Promising Anti-Staphylococcus aureus. Mini Rev Med Chem. 2019;19:923-932.
18. Jaymand K, Rezaei MB, Asgari F, MeshkatiZadeh S. Chemical Composition of Chamomile Essential Oil. Matricaria chamomilla L. J Iranian Med Arom Plants Res. 2001;10;105-125.
19. Ayogi F, Barzegar M, Sahari MA, Badi H. Chemical Compounds of Tarragon (Artemisia dracunculus L.) and Chamomile Essential Oil (Matricaria chamomilla L.) Essential Oils and Evaluation of their Antioxidant Activity. Intern J Agricult Sci Technol. 2010;13:79-88.
20. Rezaee MB, Jaimand K, Mozaffrian V. Essential oil composition of Anthemis coelopoda Boiss. Iranian J Med Aromat Plants. 2009;24:271-277.
21. Foster S. Chamomile. Botanical series. American Botanical Council, Austin. Texas. 1991;307.
22. Omidbaigi R. Production and processing of medicinal plant. Astan ghods razavi press. 2000;3:14-36.
23. Koppel T, Arak E, Türi E. Taimse päritoluga ravimite antimikroobse toime uurimine I. Eesti Rohuteadlane 1993;3:107-9.
24. Grgesina D, Mandic ML, Karuza L, Klapec. Chemical composition of different parts of Matricaria chamomilla. Prehrambeno Tehnol Biotehnol Rev. 1995;33:111-113.
25. Ghanavati M. Study of salinity effect on some growth characters of two matricaria spices. A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, Department of plant breeding, Shahrekord University. 2007;25-68.
26. Ghanavati M, Hooshmand S, Zeinali H, Ebrahimpour F. Chemical composition of chamomile essential oil (Matricaria recutita L.) in central and southern Iran. J Med Plants. 2010;9:34:108-102.
27. Abd El-Wahed MSA, Krima M, Gamal ED. Stimulalation of growth, flowering, biochemical constituents and essential oil of chamomile plant (Chamomilla recutita L, RAUSCH) with spermidine and stigmasterol application, Bulg. J Plant Physiol. 2004;30:89-102.
28. Anne O, Tiiu K, Kailas W. Volatile constituents of matricaria recutita l. from Estonia, Proc. Estonian Acad Sci Chem. 2001;50:1:39-45.
29. Ghanavati M, Hushmand S, Zeinali H, Ebrahimpour F. Chemical composition of the essential oils of Matricaria recutita L. belonging to central and south parts of Iran. J Med Plants. 2010;34:102-8.
30. Kalodera A, Bohn KH, Schultze W. The essential oil of Tanacetum parthenium L. Planta Med. 2006;55:489-93.