Effects of Atriplex hortensis Hydroalcoholic Extract on Phenyl-hydrazine Induced Hemolytic Anemia in Rat

Document Type : Research Paper

Authors

1 Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran

2 Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran

3 Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran

Abstract

Hemolytic anemia is a hematological disorder occurs owing to the excessive hemolysis of red blood cells. Atriplex hortensis L. leaves are traditionally used for prevention and treatment of anemia. The present study was performed to assess the effects of A. hortensis extract on phenyl-hydrazine induced hemolytic anemia in rat. Thirty-six, six months old, male Wistar rats were randomly divided into six groups of 6 rats each. One group received normal saline and other 5 groups received 40 mg/kg phenyl-hydrazine (PHZ) intraperitoneally at baseline and on day 33 for induction of hemolytic anemia. Four PHZ treated groups received daily oral doses of 50, 100, 200, and 300 mg/kg/day A. hortensis extract for 31 days and one PHZ treated group kept as control received saline accordingly. Blood superoxide dismutase (SOD) activity and blood hematological parameters such as hematocrit (HCT), red blood cell (RBC), reticulocyte (Ret) and hemoglobin (HGB) were determined in all groups after 34 days of the study. The blood RBC, HGB and HCT parameters were significantly lowers and Ret increased significantly in PHZ control group compared with the normal saline treated group. In A. hortensis extract (100, 200, and 300 mg/kg/day) treated groups the blood HCT, RBC and HGB parameters were significantly increased and Ret decrease, compared with PHZ control group, the blood SOD enzyme activity was significantly improved in all A. hortensis treated groups compared with PHZ control group. A. hortensis extract improved blood SOD activity and blood HCT, RBC, Ret and HGB parameters indicating inhibition of hemolytic anemia induced by phenyl-hydrazine in rat.

Keywords

References

  1. Beutler E, Waalen J. The definition of anemia: what is the lower limit of normal of the blood hemoglobin concentration? Blood. 2006;107:1747-1750.
  2. Vlagopoulos PT, Tighiouart H, Weiner DE, Griffith J, Pettitt D, Salem DN, Levey AS, Sarnak MJ. Anemia as a risk factor for cardiovascular disease and all-cause mortality in diabetes: the impact of chronic kidney disease. J American Soci Nephrology. 2005;16:3403-3410.
  3. Al-Ahmad A, Rand WM, Manjunath G, Konstam MA, Salem DN, Levey AS, Sarnak MJ. Reduced kidney function and anemia as risk factors for mortality in patients with left ventricular dysfunction. J American College Cardiol. 2001;38:955-962.
  4. Benoist Bd, McLean E, Egll I, Cogswell M. Worldwide prevalence of anaemia 1993-2005: WHO global database on anaemia. Worldwide prevalence of anaemia 1993-2005: WHO global database on anaemia. 2008.
  5. Naghavi M, Abolhassani F, Pourmalek F, Lakeh MM, Jafari N, Vaseghi S, Hezaveh NM, Kazemeini H. The burden of disease and injury in Iran 2003. Populat Health Metr. 2009;7:9.
  6. Fauci AS. Harrison's principles of internal medicine: Mcgraw-hill New York. 1998.
  7. Piva E, Brugnara C, Spolaore F, Plebani M. Clinical utility of reticulocyte parameters. Clinic Laborator Med. 2015;35:133-163.
  8. Fibach E, Rachmilewitz E. The role of oxidative stress in hemolytic anemia. Current Molecul Med. 2008;8:609-619.
  9. Friedman JS, Rebel VI, Derby R, Bell K, Huang T.T, Kuypers FA, Epstein CJ, Burakoff SJ. Absence of mitochondrial superoxide dismutase results in a murine hemolytic anemia responsive to therapy with a catalytic antioxidant. J Experiment Med. 2001;193:925-934.
  10. Ashour TH. Phenylhydrazine‑induced hemolytic anemia in rats. Res J Med Sci. 2014;8:67-72.
  11. Thakur A, Kumar P, Chand D. Superoxide Dismutase and Oxidative Stress: Elucidation of Antiaging and Antioxidative Effect on Mammalian Cell Lines. J Adv Microbiol. 2017;3:54-59.
  12. Beutler E. Drug-induced hemolytic anemia. Pharmacol Rev. 1969;21:73-103.
  13. Heidari A ZS, Heidari G. Ethnobotanical survey of Namarestagh summer rangelands, Amol (Mazandaran). J Islamic Iranian Traditional Med. 2015;5:330-339.
  14. Bylka W, Stobiecki M, Frański R. Sulphated flavonoid glycosides from leaves of Atriplex hortensis. Acta Physiol Plant. 2001;23:285-290.
  15. Awaad AS, Maitland D, Donia AERM, Alqasoumi SI, Soliman GA. Novel flavonoids with antioxidant activity from a Chenopodiaceous plant. Pharma Biol. 2012;50:99-104.
  16. Wright K, Pike O, Fairbanks D, Huber C. Composition of Atriplex hortensis, sweet and bitter Chenopodium quinoa seeds. J Food Sci. 2002;67:1383-1385.
  17. Carlsson R. Quantity and Quality of Leaf Protein Concentrates from Atriplex hortensis L., Chenopodium quinoa Willd. and Amaranthus caudatus L, Grown in Southern Sweden. Acta Agri Scandinavica. 1980;30:418-426.
  18. Atanassova M, Aslam M, Sharma S. Studies on nutritional facts of spring herbs collected from Bulgarian market. J Pub Health Catalog. 2018;1:15-20.
  19. Han J, Weng X, Bi K. Antioxidants from a Chinese medicinal herb–Lithospermum erythrorhizon. Food Chem. 2008;106:2-10.
  20. Gutfinger T. Polyphenols in olive oils. J American Oil Chem Soci. 1981;58:966-968.
  21. Yoo KM, Lee CH, Lee H, Moon B, Lee CY. Relative antioxidant and cytoprotective activities of common herbs. Food Chem. 2008;106:929-936.
  22. Chikhi I, Allali H, Dib MEA, Medjdoub H, Tabti B. Antidiabetic activity of aqueous leaf extract of Atriplex halimus L.(Chenopodiaceae) in streptozotocin-induced diabetic rats. Asian Pacific J Tropic Dis. 2014;4:181.
  23. Goudarzvand M, Afraei S, Yaslianifard S, Ghiasy S, Sadri G, Kalvandi M, Alinia T, Mohebbi A, Yazdani R, Azarian SK. Hydroxycitric acid ameliorates inflammation and oxidative stress in mouse models of multiple sclerosis. Neural Regenerat Res. 2016;11:1610.
  24. Hertog MG, Kromhout D, Aravanis C, Blackburn H, Buzina R, Fidanza F, Giampaoli S, Jansen A, Menotti A, Nedeljkovic S. Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study. Archiv Int Med. 1995;155:381-386.
  25. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J Nutr Biochem. 2002;13:572-584.
  26. Cook N, Samman S. Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr Biochem. 1996;7:66-76.
  27. Mojisola OC, Adebolu EA, Alani DM. Antisickling properties of Carica papaya Linn. J Natural Product. 2008;1:56-58.
  28. Mpiana P, Mudogo V, Kabangu Y, Tshibangu D, Ngbolua K, Atibu E, Mangwala K, Mbala M, Makelele L, Bokota M. Antisickling activity and thermostability of anthocyanins extract from a congolese plant, Hymenocardia acida Tul.(Hymenocardiaceae). Int J Pharmacol. 2009;5:65-70.
  29. Natarajan KS, Narasimhan M, Shanmugasundaram KR, Shanmugasundaram E. Antioxidant activity of a salt–spice–herbal mixture against free radical induction. J Ethnopharma. 2006;105:76-83.
  30. Imaga N. The use of phytomedicines as effective therapeutic agents in sickle cell anemia. Sci Res Essay. 2010;5:3803-3807.
  31. Wambebe CO, Bamgboye EA, Badru BO, Khamofu H, Momoh JA, Ekpeyong M, Audu BS, Njoku SO, Nasipuri NR, Kunle OO. Efficacy of niprisan in the prophylactic management of patients with sickle cell disease. Current Therap Res. 2001;62:26-34.
  32. Wambebe C, Khamofu H, Momoh J, Ekpeyong M, Audu B, Njoku O, Bamgboye E, Nasipuri R, Kunle O, Okogun J. Double-blind, placebo-controlled, randomised cross-over clinical trial of NIPRISAN® in patients with Sickle Cell Disorder. Phytomedicine. 2001;8:252-261.
  33. Lee HW, Kim H, Ryuk JA, Kil K.J, Ko BS. Hemopoietic effect of extracts from constituent herbal medicines of Samul-tang on phenylhydrazine-induced hemolytic anemia in rats. Int J Clin Experiment Pathol. 2014;7:6179.
Journal of Medicinal plants and By-product
Volume 11, Special
April 2022
Pages 37-41

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