Phytochemicals and Biological Activities of Froriepia subpinnata (Ledeb.) Baill. Extracts

Document Type : Research Paper

Authors

Department of Chemistry, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran

Abstract

Nowadays, the use of herbal extracts for preparing various drugs is increasing because of the compatibility of the natural compounds present in the extracts with the human body. To this end, in this project, we studied Froriepia subpinnata (Ledeb.) Baill. in terms of its natural constituents and biological activities. The chemical composition of the hexane extract of F. subpinnata aerial parts was evaluated by the GC/MS analysis and 21 compounds (80.60%) of the total, including phytosterols and hydrocarbons, were identified. Also besides, a significant amount of flavonoids in the methanol extract of F. subpinnata (27.235 ± 0.048μg/mL) was estimated by the AlCl3 colorimetric method. Two flavonoids: rutin and catechin were identified in the methanol extract of F. subpinnata by HPLC analysis. The concentration of the flavonoids was evaluated (23.61 and 6.95) mg/g respectively. Antioxidant activity of the methanol extract of the species was also evaluated by the DPPH (1,1-diphenyl-2-picryl-hydrazyl) assay. We also investigated the antibacterial activity of methanol extract of F. subpinnata aerial parts against two bacteria Staphylococcus aureus and Escherichia coli by the MIC (Minimum Inhibition Concentration) method. The MIC values for S. aureus and E. coli were evaluated (0.031 and 1.0) mg/mL respectively. Our study shows that F. subpinnata possesses a variety of useful natural constituents, which makes it a promising plant for agricultural and medicinal uses.

Keywords

References

1. Kour H, Agarwal S, Raina R, Verma PK. Phytochemical ingredients and pharmacological potential of Calendula officinalis Linn. Pharm Biomed Res. 2018;4:1-17.
2. Pagare S, Bhatia M, Tripathi N, Pagare S, Bansal YK. Secondary metabolites of plants and their role: Overview. Curr Trends Biotechnol Pharm. 2015;9:293-304.
3. Panche AN, Diwan AD, Chandra SR. Flavonoids: An overview. J Nutr Sci. 2016;5:e47.
4. Havsteen BH. The biochemistry and medical significance of the flavonoids. Pharmacol Ther. 2002;96:67-202.
5. Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. Sci World J. 2013;2013:162750.
6. Leute GH. Froriepia. In: Hedge IC, Lamond JM and Rechinger KH,  Flora Iranica, Umbelliferae. Vol. Graz, Austria: Academische Druck and Verlagsanstalt. 1987;297.
7. Mozaffarian V. Umbelliferae. In: Assadi M, Khatamsaz M and Maasoumi AA,  Flora of Iran. Vol 54. Tehran, Iran: Research Institute of Forests and Rangelands Publication. 2007.
8. Seyedabadi MM, Amirabadi AA, Taheri A and Kashani Nejad M. The effect of infrared drying on the drying kinetics and leaf color index of Froriepia subpinnata. Innovative Food Technol. 2016;13:45-57.
9. Morteza-Semnani K, Saeedi M, Akbarzadeh M. The essential oil composition of Froriepia subpinnata (Ledeb.) Baill. J Essen Oil Res. 2009;21:127-128.
10. Rustaiyan A, Mojab R, Kazemie-Piersara M, Bigdeli M, Masoudi S, Yari M. Essential oil of Froriepia subpinnata(Ledeb.) Baill. from Iran. J Essen Oil Res. 2001;13:405-406.
11. Mohammadzadeh M, Mahmoudi R, Ghajarbeygi P. Evaluation of chemical composition and antibacterial properties of Froriepia subpinnta essential oils from guilan region: Before and after flowering. J Essen Oil Bearing Plants. 2018;21:1119-1127.
12. Mirzania F, Sarrafi Y, Moridi Farimani M. Comparative Evaluation of Chemical Compositions and Biological Activities of Wild and Cultivated Froriepia subpinnata L. Essential Oils. J Agric Sci Technol. 2019;21:331-340.
13. Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Eslami S, Bekhradnia AR. Mineral elements and antioxidant activity of three locally edible and medicinal plants in Iran. Asian J Chem. 2010;22:6257-6266.
14. Nabavi SM, Nabavi SF, Ebrahimzadeh MA, Eslami B. In vitro antioxidant activity of pyrus boissieriana, diospyros lotus, eryngium caucasicum and Froriepia subpinnata. J Rafsanjan Univ Med Sci. 2009;8:139-150.
15. Latifi Z, Sadeghi F, Azizi J, Danshniya M. Measuring of phytochemical and antioxidant compounds and identification of main phenolic compound in Anarijeh plant (Froriepia subpinnata) extract by RPHPLC method. In: 13th International Conference on Engineering and Technology. Oslo, Norway. 2019.
16. Jorkesh A, Hamidoghli Y, Olfati J, Samizadeh H, Bakhshi D, Palá-Paúl J. Morphological and biochemical variability of Froriepia. Int J Veg Sci. 2019;26:262-274.
17. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colometric methods. J Food Drug Analysis. 2020;10.
18. Mensor LL, Menezes FS, Leitao GG, Reis AS, dos Santos TC, Coube CS, Leitao SG. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res. 2001;15:127-130.
19. Jorgensen JH, Turnidge JD. Susceptibility test methods: dilution and disk diffusion methods. In: Murray PR, Baron EJ, Jorgensen JH, Landry ML and Pfaller MA,  Manual of Clinical Microbiology. Vol 1. Washington DC: American Society for Microbiology. 2007;1152-1172.
20. Rudkowska I, AbuMweis SS, Nicolle C, Jones PJ. Cholesterol-lowering efficacy of plant sterols in low-fat yogurt consumed as a snack or with a meal. J Am Coll Nutr. 2008;27:588-595.
21. Kaur N, Chaudhary J, Jain A, Kishore L. Stigmasterol: A comprehensive review. Int J Pharm Sci Res. 2011;2:2259.
22. Suttiarporn P, Chumpolsri W, Mahatheeranont S, Luangkamin S, Teepsawang S, Leardkamolkarn V.  Structures of phytosterols and triterpenoids with potential anti-cancer activity in bran of black non-glutinous rice. Nutrients. 2015;7:1672-1687.
23. Kangsamaksin T, Chaithongyot S, Wootthichairangsan C, Hanchaina R, Tangshewinsirikul C, Svasti J. Lupeol and stigmasterol suppress tumor angiogenesis and inhibit cholangiocarcinoma growth in mice via downregulation of tumor necrosis factor-alpha. PLOS ONE. 2017;12:e0189628.
24. Grattan BJ, Jr. Plant sterols as anticancer nutrients: evidence for their role in breast cancer. Nutrients. 2013;5:359-387.
25. Baskar AA, Al Numair KS, Gabriel Paulraj M, Alsaif MA, Muamar MA, Ignacimuthu S. beta-sitosterol prevents lipid peroxidation and improves antioxidant status and histoarchitecture in rats with 1,2-dimethylhydrazine-induced colon cancer. J Med Food. 2012;15:335-343.
26. Mano Bala J, Jannet Vennila J, Ganesh S, Sing S. Analysis of active components in Acanthus ilicifolius leaves for their potential chemo protective activity against hepatocellular carcinoma: An in silico approach. J Pharm Res. 2011;4:1809-1812.
27. Ganesh S, Vennila JJ. Phytochemical analysis of Acanthus ilicifolius and avicennia officinalis by GC-MS. Res J Phytochem. 2011;5:60-65.
28. Praveena A, Ramkumar G, Sanjayan KP. Phytochemical screening of the extract of the root-bark of Morinda tinctoria (Rubiaceae) for secondary metabolites. Res J Pharm Technol. 2012;5:83-87.
29. Slamova K, Kapesova J, Valentova K. "Sweet flavonoids": Glycosidase-catalyzed modifications. Int J Mol Sci. 2018;19.
30. Moghaddasian B, Eradatmand AD and Alaghemand A. Simultaneous determination of rutin and quercetin in different parts of Capparis spinosa. Bull Environ Pharmacol Life Sci. 2013;2:35-38.
31. Khalesi S, Sun J, Buys N, Jamshidi A, Nikbakht-Nasrabadi E, Khosravi-Boroujeni H. Green tea catechins and blood pressure: A systematic review and meta-analysis of randomised controlled trials. Eur J Nutr. 2014;53:1299-1311.
32. Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial activities of flavonoids: Structure-activity relationship and mechanism. Curr Med Chem. 2015;22:132-149.
33. Cheng H, Wang L, Mollica M, Re AT, Wu S, Zuo L. Nitric oxide in cancer metastasis. Cancer Lett. 2014;353:1-7.
34. Sun GY, He Y, Chuang DY, Lee JC, Gu Z, Simonyi A, Sun AY. Integrating cytosolic phospholipase A (2) with oxidative/nitrosative signaling pathways in neurons: A novel therapeutic strategy for AD. Mol Neurobiol. 2012;46:85-95.
35. Sack MN, Fyhrquist FY, Saijonmaa OJ, Fuster V, Kovacic JC. Basic biology of oxidative stress and the cardiovascular system: Part 1 of a 3-part series. J Am Coll Cardiol. 2017;70:196-211.
36. Dennis JM and Witting PK. Protective role for antioxidants in acute kidney disease. Nutrients. 2017;9.
37. Kawakami T, Koike A, Amano F. Sodium bicarbonate regulates nitric oxide production in mouse macrophage cell lines stimulated with lipopolysaccharide and interferon gamma. Nitric Oxide. 2018;79:45-50.
38. Singh R, Devi S, Gollen R. Role of free radical in atherosclerosis, diabetes and dyslipidaemia: Larger-than-life. Diabetes Metab Res Rev. 2015;31:113-126.
39. Lv W, Booz GW, Fan F, Wang Y, Roman RJ. Oxidative stress and renal fibrosis: Recent insights for the development of novel therapeutic strategies. Front Physiol. 2018;9:105.
40. Varijakzhan D, Chong CM, Abushelaibi A, Lai KS, Lim SE. Middle Eastern plant extracts: An alternative to modern medicine problems. Molecules. 2020;25.
Journal of Medicinal plants and By-product
Volume 10, Issue 1
April 2021
Pages 109-115

Files

Share

How to cite

Statistics