Effects of Drying Methods on the Essential Oil Content and Composition of Nepeta binaludensis Jamzad

Document Type : Research Paper

Authors

1 Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Abstract

Nepeta binaludensis Jamzad is an endemic and rare perennial plant belonging to the Lamiaceae family which grows in a limited area in Binaloud Mountain in northeast of Iran. In order to study the effect of drying methods on its essential oil content and composition, the aerial parts were collected in flowering stage from its habitat at altitude of 2650 m above sea level in July 2018. Seven drying methods as sun drying, shade drying, oven drying at 30oC, 40oC and 50oC and microwave drying (180W and 360W) were applied in three replications. The essential oils of all samples were obtained using a Clevenger type apparatus during 3 h. The essential oils were analyzed using gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS). Data statistically analyzed using one way ANOVA (CRD) with three replications and mean comparison were made using the Tukey method. The highest oil yield was obtained in oven drying 50 °C (2.16%) followed by shade drying (1.96%). In contrast, the lowest values of essential oil content, 1.09 and 0.65%, were obtained in sun drying and microwave 360W, respectively. Twenty-four compounds were identified in the essential oils of N. binaludensis with various drying methods with neptalactone (23.1-54.0%), 1,8-cineol (25.5-51.0%) and myrcene (2.0-4.0%) as major components. The highest percentage of 1,8-cineole (51%) was obtained from sample dried in oven 50 °C, while, the highest amount of neptalactone (54.0%) was obtained from sample dried by the microwave 180 W. The results demonstrated that drying the aerial parts of N. binaludensis in the shade and oven 50oC are the best optimal methods to obtain the highest oil yield, while drying in microwave at 180 W is recommended to enhance higher neptalactone content in the oil.

Keywords

References

1. Jamzad Z, Chase MW, lngrouille M, Simmonds MS, Jalili A. Phylogenetic relationships in Nepeta L. (Lamiaceae) and related genera based on ITS sequence data. Taxon. 2003a;52:21-32.
2. Jamzad Z. Flora of Iran, No.76: Lamiaceae Family. Agricultural Research, Education and Extension Organization, Research Institute of Forests and Rangelands, Tehran. 2012, pp. 801-880. (In Persian).
3. Mozaffarian V. Identification of Medicinal and Aromatic Plants of Iran. Farhang Moaser Press, Tehran, Iran. 2013. (In Persian).
4. Jamzad Z, Grayer RJ, Kite GC, Simmonds MS, Ingrouille M, Jalili A. Leaf surface flavonoids in Iranian species of Nepeta (Lamiaceae) and some related genera. Biochem Syst Ecol. 2003b;31:587-600.
5. Zargari A. Medicinal Plants, vol. 4. Tehran University Press, Tehran, Iran. 2011. (In Persian).
6. Asgarpanah J, Sarabian S, Ziarati P. Essential oil of Nepeta genus (Lamiaceae) from Iran: a review. J Essent Oil Res. 2014;26:1-12.
7. Formisano C, Rigano D. Senatore F. ChemInform Abstract: Chemical Constituents and Biological Activities of Nepeta Species. Chem Biodiver. 2011;8:1783-818.
8. Nadjafi F, Koocheki A, Honermeier B, Asili J. Autecology, ethnomedicinal and phytochemical studies of Nepeta binaludensis Jamzad a highly endangered medicinal plant of Iran. J Essent Oil Bear Plants. 2009;12:97-110.
9. Rustaiyan A, Nadji K. Composition of the essential oils of Nepeta ispahanica Boiss. and Nepeta binaludensis Jamzad from Iran. Flavour Fragrance J. 1999;14:35-37.
10. Nadjafi F, Koocheki A, Moghaddam PR, Honermeier B. First experiments on cultivation of Nepeta binaludensis Jamzad, an example of domestication of a highly endangered medicinal plant of Iran. J Med Spice Plants. 2012;17:64-71.
11. Ghasemi Pirbalouti A, Oraie M, Pouriamehr M, Babadi ES. Effects of drying methods on qualitative and quantitative of the essential oil of Bakhtiari savory (Satureja bachtiarica Bunge.). Indust Crops Products. 2013;46:324-327.
12. Rahmati M, Azizi M, Ebadi MT, Hasanzadeh Khayyat, M. Study on the effects of different drying methods on weight loss rate, essential oil and chamazolene contents of chamomile (Matricaria recutita CV. Germania (diploid)) flowers. J Hortic Sci. 2010;24:29-37. (In Persian).
13. Omidbeigi R, Sefidkon F, Kazemi, F. Influence of drying methods on the essential oil content and composition of roman chamomile. Flavour Fragrance J. 2004;19:196-198.
14. Ebadi MT, Azizi M, Sefidkon F, Ahmadi N. Influence of different drying methods on drying period, essential oil content and composition of Lippia citriodora Kunth. J Applied Res Med Aroma Plants. 2015;2:182-187.
15. Venskutonis PR. Effect of drying on the volatile constituents of thyme (Thymus vulgaris L.) and sage (Salvia officinalis L.). Food Chem. 1997;59:219-227.
16. Soysal Y, Öztekin S, Eren Ö. Microwave Drying of Parsley: Modeling, Kinetics, and Energy Aspects. Biosystems Engineering. 2006;93:403-413.
17. Azizi M, Rahmati M, Ebadi MT, Hasanzadeh Khayyat M. The effects of different drying methods on weight loss rate, essential oil and chamazolene contents of chamomile (Matricaria recutita) flowers. Iranian J Med Aroma Plants Res. 2009;25:182-192. (In Persian).
18. Díaz-Maroto MC, Pérez-Coello MS, González Viñas MA, Cabezudo MD. Influence of Drying on the Flavor Quality of Spearmint (Mentha spicata L.). J Agric Food Chem. 2003;51:1265-1269.
19. Tarakemeh A, Abutalebi A. Effect of Drying Method on the Essential Oil Quantity of Basil (Ocimum basilicum L.). J Essent Oil Bear Plants. 2012;15:503-505.
20. Blanco MCSG, Ming LC, Marques MOM, Bovi OA. Drying temperature effects in rosemary essential oil content and composition. Acta Hort. 2002;569:99-103
21. Fathi E, Sefidkon F, Khaniki GB, Abravesh Z, Assareh MH. The effects of drying and distillation methods on essential oil content and composition of Eucalyptus largiflorens. Iranian J Med Aroma Plants. 2009;25:64-74. (In Persian).
22. Kayhani A, Sefidkon F, Monfared A. The effect of drying and distillation methods on essential oil content and composition of Satureja sahendica Bornm. Iranian J Med Aroma Plants. 2014;30:239-248. (In Persian).
23. Mohtashami S, Babablar M, Ebrahim Zade Moosavi, S M, Mir Jalili MH, Adib J. The Effect of Growing Conditions and Different Drying Methods on Drying Time, Essential Oil Content, Color Characteristics and Microbial Load of Dracocephalum moldavica L. Iranian J Hortic Sci. 2012;43:243-254. (In Persian).
24. Mohammadizad HA, Mehrafarin A, Naghdi Badi H. Qualitative and quantitative evaluation of essential oil of Catnip (Nepeta cataria L.) under different drying conditions. J Med Plants by products. 2017;16:8-20.
25. Hadjibagher Kandi MN, Sefidkon F. The Influense of Drying Methods on Essential Oil Content and Composition of Laurus nobilis L. J Essent Oil Bear Plants.  2011;14:302-308.
26. Sefidkon F, Abbasi K, Khaniki GB. Influence of drying and extraction methods on yield and chemical composition of the essential oil of Satureja hortensis. Food Chem. 2006;99:19-23.
27. Ebadi MT, Rahmati M, Azizi M, Hassanzadeh Khayyat M, Dadkhah A. The effects of different drying methods on drying time, essential oil content and composition of basil (Ocimum basilicum L.). Iranian J Med Aroma plants. 2013;29:425-437. (In Persian).
28. Ahmed A, Ayoub K, Chaima AJ, Hanaa L, Abdelaziz, C. Effect of drying methods on yield, chemical composition and bioactivities of essential oil obtained from Moroccan Mentha pulegium L. Biocatalysis Agric Biotech. 2018;16:638-643.
29. Rahimmalek M, Goli S. Evaluation of six drying treatments with respect to essential oil yield, composition and color characteristics of Thymys daenensis subsp. daenensis. Celak leaves. Indust Crops Products. 2013;42:613–619.
30. Ghani A, Azizi M. The effect of different drying methods on quantity and quality characteristics of five yarrow species (Achillea). The plant production (Sci J Agric). 2009;32:1-11. (In Persian).
31. Roshanak S, Rahimmalek M, Hossein Goli SA. Evaluation of seven different drying treatments in respect to total flavonoid, phenolic, vitamin C content, chlorophyll, antioxidant activity and color of green tea (Camellia sinensis) leaves. J Food Sci Technol. 2016;53:721-729.
32. Rezvani Moghaddam PR, Ghani A, Rahmat M, Mohtashami S. Effects of different drying methods on drying time and some active substances of two populations of Tarragon (Artemisia dracunculus L.). Iranian J Med Aroma Plants. 2013;29:460-475. (In Persian).
33. Asekun OT, Grierson DS, Afolayan AJ. Effects of drying methods on the quality and quantity of the essential oil of Mentha longifolia L. subsp. Capensis. Food Chem. 2007;101:995-998.
34. Rahimi A, Farrokhi E. Evaluation of the Effect of Different Drying Methods on Antioxidant and Phytochemical Activity of Essential oil of Origanum vulgare L. subsp. gracile. Eco-phytochemical J Med Plants. 2019;7:15-27. (In Persian).
Journal of Medicinal plants and By-Products
Volume 9, Issue 2
September 2020
Pages 207-214

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