The Biochemical Properties of Zarrin-Giah (Dracocephalum kotschyi Boiss) Medicinal Plant Affected by Seaweed Extract and Amino Acid Spraying

Authors

Department of Horticulture, Faculty of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Abstract

The use of medicinal plants, as favorite sources of different metabolites for human health, has become more popular, worldwide. It is accordingly important to find methods, which may enhance the growth and quality of medicinal plants including the important medical Zarrin-giah (Dracocephalum kotschyi Boiss). The plant is used for the treatment of different diseases including cancer and Alzheimer. In the presented research, the effects of different biostimulants including seaweed extracts (Wuxal Ascofol, 1 (A1), 2 (A2), and 3 (A3) g/L) and algaton amino acid (1 (J1), 2 (J2), and 3 (J3) g/L) on the biochemical properties of Zarrin-giah were investigated in the greenhouse. Different plant biochemical properties including chlorophyll contents (a, b, and total), carotenoid, flavonoid, total phenol, antioxidant activity and leaf greenness were determined. The results indicated the positive effects of the experimental treatments on the quality of Zarrin-giah by improving the biochemical properties of the plant. The algal and the amino acid treatments significantly increased the chlorophyll a content of the plant, compared with the control treatment (0.34 mg/g). This was also the case for chlorophyll b and total chlorophyll. Although there was not much difference among the algal and amino acid treatments, on the content of carotenoid, treatments A1 (0.118 mg/g) and J1 (0.116 mg/g) resulted in the higher carotenoid content, significantly different from the other treatments including control (0.102 mg/g). The highest and significantly different flavonoid contents were resulted by the A2 (0.28%) and the J2 (0.276%) treatments. Interestingly, the least flavonoid content was related to the A3 (0.22%) treatment, even significantly less than the control treatment (0.236%) The J3 treatment (6076.336 mg/kg) resulted in the highest and significantly different content of total phenol. However, the least contents of the total phenol were related to the A3 (3510.556 mg/kg) and the control (3637.984 mg/kg) treatments. Algal treatments were more effective on the increased activity of the antioxidants. Accordingly, treatment A3 (77.167%) resulted in the highest and significantly different activity of antioxidant, followed by A2 (58.974%), and the least one was resulted by control (27.473%). The highest leaf greenness (SPAD) was related to the A2 treatment (39.933), significantly higher than the other treatments including J2 (31.733), J3 (27.033) and A1 (20.067). If the proper concentrations of biostimulants including seaweed extract and amino acid treatments tested in this research are used, it is possible to enhance the quality of Zarrin-giah, because at the higher rates, they may not favorably affect the plant quality. 

Keywords

References

1. Mohammadi M, Asadi-Gharneh HA. How the morphological properties of Mentha longifolia.(L.) Huds. may be affected by geographical differences. J Photochem Photobiol B. 2018;178:237-242.
2. Mostafavi S, Asadi-Gharneh HA, Miransari M. The phytochemical variability of fatty acids in basil seeds (Ocimum basilicum L.) affected by genotype and geographical differences. Food Chem. 2019;276:700-706.
3. Jalaei Z, Fattahi M, Aramideh S. Allelopathic and insecticidal activities of essential oil of Dracocephalum kotschyi Boiss. from Iran: A new chemotype with highest limonene-10-al and limonene. Industrial Crops and Products. 2015;73:109-117.
4. Fattahi M, Bonfill M, Fattahi B, Torras-Claveria L, Sefidkon F, Cusido RM,  Palazon J. Secondary metabolites profiling of Dracocephalum kotschyi Boiss at three phenological stages using uni-and multivariate methods. J Appl Res Med Aromat Plants. 2016;3:177-185.
5. Kamali H, Khodaverdi E, Hadizadeh F, Ghaziaskar SH. Optimization of phenolic and flavonoid content and antioxidants capacity of pressurized liquid extraction from Dracocephalum kotschyi via circumscribed central composite. J Supercrit Fluid. 2016;107:307-314.
6. Manea AI, Abbas KAU. Influence of seaweed extract, organic and inorganic fertilizer on growth and yield broccoli. Int J Veg Sci. 2018;24:550-556.
7. Hidangmayum A, Sharma R. Effect of different concentrations of commercial seaweed liquid extract of Ascophyllum nodosum as a plant bio stimulant on growth, yield and biochemical constituents of onion (Allium cepa L.). J Pharmacogn Phytochem. 2017;6:658-663.
8. Hernández-Herrera RM, Santacruz-Ruvalcaba F, Briceño-Domínguez DR, Di Filippo-Herrera DA, Hernández-Carmona G. Seaweed as potential plant growth stimulants for agriculture in Mexico. Hidrobiológica. 2018;28:129-140.
9. Kalhor MS, Aliniaeifard S, Seif M, Asayesh EJ, Bernard F, Hassani B Li T. Enhanced salt tolerance and photosynthetic performance: Implication of ɤ-amino butyric acid application in salt-exposed lettuce (Lactuca sativa L.) plants. Plant Physiol Bioch. 2018;130:157-172.
10. Bashir A, Rizwan M, Ali S, Rehman MZ, Ishaque W, Riaz MA, Maqbool A. Effect of foliar-applied iron complexed with lysine on growth and cadmium (Cd) uptake in rice under Cd stress. Environ Sci Pollut Res. 2018;25:20691-20699.
11. Hussain A. Ali S, Rizwan M, Rehman, MZ, Hameed A, Hafeez F, Alamri SA, Alyemeni MN, Wijaya L. Role of zinc–lysine on growth and chromium uptake in rice plants under Cr stress. J Plant Growth Regul. 2018;37:1413-1422.
12. Michalak I, Tuhy Ł, Chojnacka K. Seaweed extract by microwave assisted extraction as plant growth biostimulant. Open Chem. 2015;13.
13. Hernández-Herrera RM, Santacruz-Ruvalcaba F, Zañudo-Hernández J, Hernández-Carmona G. Activity of seaweed extracts and polysaccharide-enriched extracts from Ulva lactuca and Padina gymnospora as growth promoters of tomato and mung bean plants. J Appl Phycol. 2016;28:2549-2560.
14. Arioli T, Mattner SW. Winberg PC. Applications of seaweed extracts in Australian agriculture: past, present and future. J Appl Phycol. 2015;27:2007-2015.
15. Van Oosten MJ, Pepe O, De Pascale S, Silletti S, Maggio A. The role of biostimulants and bioeffectors as alleviators of abiotic stress in crop plants. Chem Biol Technol Agric. 2017;4:1-5.
16. Yildiztekin M, Tuna AL, Kaya C. Physiological effects of the brown seaweed Ascophyllum nodosum) and humic substances on plant growth, enzyme activities of certain pepper plants grown under salt stress. Acta Biol Hung. 2018;69:325-335.
17. Weber N, Schmitzer V, Jakopic J, Stampar F. First fruit in season: seaweed extract and silicon advance organic strawberry (Fragaria × ananassa Duch.) fruit formation and yield. Sci Hortic. 2018;242:103-109.
18. Maziarek A, Parylak D, Wacławowicz R. The effect of biostymulants and stubble crop on weed infestation of short-term spring wheat monoculture. Prog Plant Prot. 2015;55:170-176.
19. Arnon DI. Copper enzymes in isolated chloroplasts: poly phenol oxidase in Beta vulgaris. Plant Physiol. 1949;24:1-15.
20. Lichtenthder HK. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Method Enzymol. 1987;148:350-382.
21. Krizek DT, Britz SJ, Mirecki R M. Inhibitory effects of ambient levels of solar UV-A and UV-B radiation on growth of cv. new red fire lettuce. Physiol Plant. 1998;103:1-7.
22. Singleton V L, Rossi J A. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am J Enol Viticult. 1965;16:144-158.
23. Pokorny J, Yanishlieva N, Gordon M. Antioxidants in Food. Practical Applications. Woodhead Publishing. 2001. 380pp.
24. Sun T, Ho C T. Antioxidant activity of buck wheat extracts. Food Chem. 2005;90:743-749.
25. González A, Castro J, Vera J, Moenne A, Seaweed oligosaccharides stimulate plant growth by enhancing carbon and nitrogen assimilation, basal metabolism, and cell division. J Plant Growth Regul. 2013;32:443–448.
26. Hernández-Herrera RM, Santacruz-Ruvalcaba F, Ruiz-Lopez, MA, Norrie J, Hernández-Carmona G. Effect of liquid seaweed extracts on growth of tomato seedlings (Solanum lycopersicum L.). J Appl Phycol. 2014;26:619–628.
27. Latique S, Chernane H, Mansori M, El Kaoua M. Seaweed liquid fertilizer effect on physiological and biochemical parameters of bean plant (Phaesolus vulgaris variety Paulista) under hydroponic system. Eur Sci J. 2013;9:30-37.
28. Lola-Luz T, Hennequart F,  Gaffney M. Enhancement of phenolic and flavonoid compounds in cabbage (Brassica oleraceae) following application of commercial seaweed extracts of the brown seaweed (Ascophyllum nodosum). Agr Food Sci. 2013;22:288-295.
29. Vasantharaja R, Abraham LS, Inbakandan D, Thirugnanasambandam R, Senthilvelan T, Jabeen SA, Prakash P. Influence of seaweed extracts on growth, phytochemical contents and antioxidant capacity of cowpea (Vigna unguiculata L. Walp). Biocatal Agric Biotechnol. 2019;17:589-594.
30. Chuyen HV, Eun JB. Marine carotenoids: Bioactivities and potential benefits to human health. Crit Rev Food Sci Nutr. 2017;57:2600-2610.
31. El-Aal MA, Eid RS. Effect of foliar spray with lithovit and amino acids on growth, bioconstituents, anatomical and yield features of soybean plant. Plant Biotechnol J. 2018;187-201.
32. Mukta JA, Rahman M, Sabir AA. Gupta D.R, Surovy MZ, Rahman M, Islam MT. Chitosan and plant probiotics application enhance growth and yield of strawberry. Biocatal Agric Biotechnolo. 2017;11:9-18.
Journal of Medicinal plants and By-Products
Volume 9, Issue 2
September 2020
Pages 215-225

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