Genetic Diversity of Purslane Populations of Iran Based on Some Morphological and Biochemical Traits

Document Type : Research Paper

Authors

1 Medicinal Plants Research Center, Shahed University, Tehran, Iran

2 Department of Biotechnology, Agriculture Faculty, Shahed University, Tehran, Iran

Abstract

Portulaca oleracea L. is a medicinal plant belonging to Portulacaceae family, which exhibited a wide scope of pharmaceutical properties such as pain killer, antipyretic, anti-viral and anti-fungal. The aim of the present study was evaluation of morphological diversity 18 populations of P. oleracea. The seeds of 18 populations were cultivated based on a completely randomized design with three replicates in the research farm of Medicinal Plants Research Center, Shahed University, Tehran, Iran. The morphological traits, chlorophyll and protein contents were measured in the vegetative stage. The analysis of variance showed that there was a significant difference among populations of P. oleracea in terms of the most studied morphological and biochemical traits. The highest shoot dry weight (84.84±12.70 g per plant) belonged to the Zanjan population, while the lowest shoot dry weight (18.89±1.72 g per plant) belonged to the population of Lourdgan. The correlation between most of the studied traits was positively significant. The cluster analysis of the 18 P. oleracea populations based on morphological and biochemical traits produced three main clusters. Overall, the outcomes of the present study were indicated the presence of high genetic variability among the P. oleracea populations. Our findings suggested that collection of the plants from different regions can be used for hybridization to generate useful recombinants in the segregating generations and improve breeding varieties of P. oleracea and can be utilized for preservation and maintenance the germplasm of this medicinal plant.

Keywords

References

1. Movahedian A, Ghannadi A, Vashirnia M. Hypocholesterolemic effects of purslane extract on serum lipids in rabbits fed with high cholesterol levels. Int J Pharmacol. 2007;3:285-9.
2. Banerjee G, Mukherjee A. Pharmacognostic studies on Portulaca oleracea L. leaf. J Econ Taxon Botany. 2003;19:69-77.
3. Holm LG, Plucknett DL, Pancho JV, Herberger JP. The world's worst weeds. Distribution and biology: University press of Hawaii. 609 pages. 1977.
4. Masoodi MH, Ahmad B, Mir SR, Zargar BA, Tabasum N. Portulaca oleracea L. a review. J Pharma Res. 2011;4:3044-8.
5. Zargari A. Medicinal Plants University of Tehran Press. Tehran (in Persian). 1997;3:80-8.
6. Mohammadi M, Karimizadeh R, Shefazadeh M, Sadeghzadeh B. Statistical analysis of durum wheat yield under semi-warm dry land condition. Australian J Crop Sci. 2011;5:1292-7.
7. Chahal G, Gosal S. Principles and procedures of plant breeding: Biotechnological and conventional approaches: Alpha Science Int'l Ltd. 2002.
8. Talei D, Valdiani A, Abdullah MP. Impact of protein diversification on morphometric behavior of Andrographis paniculata Nees. Plant Systematics and Evolution. 2014;300:1003-10. DOI 10.7/s00606-013-0938-z.
9. Okpul T, Mace E, Godwin I, Singh D, Wagih M. Evaluation of variability among breeding lines and cultivars of taro (Colocasia esculenta) in Papua New Guinea using ISSR fingerprinting and agro-morphological characterization. Plant Genetic Res Newsletter. 2005;143:8.
10. Kaneko Y, Bang SW. Interspecific and intergeneric hybridization and chromosomal engineering of Brassicaceae crops. Breeding science. 2014;64:14-22.
11. Van Tuyl JM, Lim K-B, editors. Interspecific hybridisation and polyploidisation as tools in ornamental plant breeding. XXI International Eucarpia Symposium on Classical versus Molecular Breeding of Ornamentals-Part I 612. 2003.
12. Alam MA, Juraimi AS, Rafii MY, Hamid AA, Arolu IW, Latif MA. Genetic diversity analysis among collected purslane (Portulaca oleracea L.) accessions using ISSR markers. Comptes rendus biologies. 2015;338:1-11.
13. Mohebodini M, Behnamian M, Dezhsetan S. Assessment of genetic diversity of purslane (Portulaca oleracea L.) accessions in Iran: University of Mohaghegh Ardabili. 2016.
14. Mosquera SME. Purslane (Portulaca oleracea L.) an excellent source of: McGill University. 2013.
15. Grubben G. Plant Resources of Tropical Africa (PROTA): Prota. 2008.
16. Matthews JF, Ketron DW, Zane SF. The biology and taxonomy of the Portulaca oleracea L.(Portulacaceae) complex in North America. Rhodora. 1993:166-83.
17. Ren S, Weeda S, Akande O, Guo Y, Rutto L, Mebrahtu T. Drought tolerance and AFLP-based genetic diversity in purslane (Portulaca oleracea L.). J Biotech Res. 2011;3:51.
18. Alam MA, Juraimi AS, Rafii M, Hamid AA, Arolu IW, Latif M. Application of EST-SSR marker in detection of genetic variation among purslane (Portulaca oleracea L.) accessions. Brazilian J Botany. 2015;38:119-29.
19. Talei D, Mihdzar AK, Khanif MY, Saad MS, Valdiani AR. Effects of different surface sterilizers on seed germination and contamination of king of bitters (Andrographis paniculata Nees.). American-Eurasian J Agric Environ Sci. 2011;10:639-43.
20. Ling Q, Huang W, Jarvis P. Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana. Photosynthesis Res. 2011;107:209-14.
21. Talei D, Valdiani A, Puad M. An effective protein extraction method for two-dimensional electrophoresis in the anticancer herb (Andrographis paniculata Nees.). Biotechnology and Applied Biochemistry. 2013;60:521-6. DOI: 10.1155/2013/319047.
22. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976;72:248-54.
23. Xu NW, Xu S, Ehlers J. Estimating the broad-sense heritability of early growth of cowpea. Inter Journal of Plant Genomics. 2009;2009.
24. Wickramasinghe P, Harrison DK, Johnston ME. Reproductive biology and intergeneric breeding compatibility of ornamental Portulaca and Calandrinia (Portulacaceae). Australian J botany. 2010;57:697-707.
25. Alam MA, Juraimi AS, Rafii M, Hamid AA, Uddin MK, Alam M, et al. Genetic improvement of Purslane (Portulaca oleracea L.) and its future prospects. Mol Biol Reports. 2014;41:7395-411.
26. Bhatti MA, Azhar F. Salt tolerance of nine Gossypium hirsutum L. varieties to NaCl salinity at early stage of plant development. Int J Agric Biol. 2002;4:544-6.
Journal of Medicinal plants and By-Products
Volume 9, Issue 2
September 2020
Pages 227-234

Files

Share

How to cite

Statistics