Genetic Variation of Shoot Yield, Essential oil and Yield Components in Four Thyme Species

Document Type : Research Paper

Authors

1 Khorasan Razavi Agricultural and Natural Resources Research and Education Center, Agricultural Research, Educa-tion and Extension Organization (AREEO), Mashhad, Iran

2 Research Institute of Forest and Rangeland, Agricultural Research and Education Organization, (AREEO), Tehran, Iran

3 University of Tehran, Tehran, Iran

Abstract

Thymus daenensis Celak, Thymus kotschyanus Boiss. & Hohen., Thymus transcaspicus Klokov and Thymus vulgaris L. are aromatic and medicinal species which due to hybridization within species and between species has high morphological diversity. A study was carried out in order to evaluate yield and yield components in 10 ecotypes of Thymus species collected from Isfahan, Markazi, Qazvin and West Azerbaijan provinces. This experiment was performed in a randomized complete block design with three replications at Research Center for Agriculture and Natural Resources Research and Education of Razavi Khorasan province. Plant height, shoot yield, 1000-seed weight, essential oils (EO) content, EO yield, number of nodes per stem, internode length and days to flowering stage were measured in all ecotypes. T. vulgaris was considered as the control. The results of analysis of variance showed that there were significant differences (p≤0.05) for days to 50 % flowering, number of nodes, internode length, plant height, stem weight, seed weight, EO content and yield among species and ecotypes within Thymus species. The highest EO content and yield among species were observed by 2.45% and 1.28 g/plant in T. vulgaris, and 2.03% and 0.84 g/plant in T.daenensis, respectively. The highest EO content was observed among ecotypes at 1.84% in T.kotschyanus, ecotype K54 and 2.32% in T.daenensis, ecotype D49. The strong and significant (P ≤ 0.01) positive correlation of EO yield was observed with EO content (r=0.88**), seed weight per plant (r=0.78**), plant height (r=0.70*), shoot yield (r=0.80**) and internode length (r=0.89**). In the principal component analysis in thyme species and ecotypes, the eigenvalues obtained from the first three main components, explain 53%, 15%, 11%, and a total of 80% of the total variance of the variables. It was concluded that the ecotypes of D49 and D72, in T.daenensis having higher EO yield were recommended for breeding improved varieties.

Keywords

References

  1. Dashti M, Kafi M, Astaraei A, Zabihi H. Investigation of yield and yield components response of Salvia leriifolia Benth. to the biological and organic Fertilizers. Arznei-Gewurzpfla. 2018;22:84-90.
  2. Tohidi B, Rahimmalek M, Trindade H. Review on essential oil, extracts composition, molecular and phytochemical properties of Thymus species in Iran. Industrial Crops Products. 2019;134:89-99.
  3. Damtie D, Braunberger C, Conrad J, Mekonnen Y, Beifuss U. Composition and hepatoprotective activity of essential oils from Ethiopian thyme species (Thymus serrulatus and Thymus schimperi). J Essen Oil Res. 2019;31:120-128.
  4. Gedikoğlu A, Sökmen M, Çivit A. Evaluation of Thymus vulgaris and Thymbra spicata essential oils and plant extracts for chemical composition, antioxidant, and antimicrobial properties. Food Sci Nutrition. 2019;7:1704-1714.
  5. Stahl-Biskup E. The chemical composition of Thymus oils: a review of the literature 1960-1989. J Essen Oil Res. 1991;3:61-82.
  6. Aminkhani A, Sharifi R, Ranjbar E, Chalabian F, Katouzian F. Antimicrobial activities and chemical constituents of essential oil extracted from stem, leaf, and flower of Thymus fedtschenkoi from Khoy. Iranian J Food Processing Preservation. 2019;e14149.
  7. Bistgani ZE, Sefidkon F. Review on ethnobotany, phytochemical, molecular and pharmacological activity of Thymus daenensis Celak. Biocatalysis Agric Biotech. 2019;101400.
  8. Arsenijević J, Drobac M, Šoštarić I, Jevđović R, Živković J, Ražić S, Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All. Industrial Crops and Products. 2019;130:162-169.
  9. Pirbalouti AG, Hashemi M, Ghahfarokhi FT. essential oil and chemical compositions of wild and cultivated Thymus daenensis Celak and Thymus vulgaris L. Industrial Crops and Products. 2013;48:43-48.
  10. Ezzatzadeh E, Pourghasem E, Sofla SFL. Chemical composition and antimicrobial activity of the volatile oils of leaf, flower, stem and root of Thymus transcaucasicus from Iran. J Essen Oil Bearing Plants. 2014;17:577-583.
  11. Bistgani ZE, Hashemi M, DaCosta M, Craker L, Maggi F, Morshedloo MR. Effect of salinity stress on the physiological characteristics, phenolic compounds and antioxidant activity of Thymus vulgaris L. and Thymus daenensis Celak. Industrial Crops Products. 2019;135:311-320.
  12. Havala S. Deciphering those dietary recommendations–How do you define moderation. Vegetarian J. 1992;8-13.
  13. Sefidkon F, Rahimi Bidgoli A. Quantitative and qualitative variation of essential oil of Thymus kotschyanus by different methods of distillation and stage of plant growth. Iranian J Med Aro plants. 2003;15:1-21.
  14. Niazian M, Sadat Noori SA, Tohidfar M, Mortazavian SMM. Essential oil yield and agro-morphological traits in some Iranian ecotypes of Ajowan (Carum copticum L.). J Essen Oil Bearing Plants. 2017;20:1151-1156.
  15. Alavi Samani SM, Ghasemi Pirbalouti A, Ataei Kachouei M, Hamedi B. The influence of reduced irrigation on herbage, essential oil yield and quality of Thymus vulgaris and Thymus daenensis. J Herbal Drugs. 2013;4:109-113.
  16. Kaveh Sh, Zeinali H, Safaei L, Madah Arefy H, Aflakian S. Comparison of morphological and phytochemical traits in different populations of Thymus kotschyanus Boiss. & Hohen. with Thymus vulgaris L. genotypes. Iranian J Med Aro Plants. 2013;29:116-129.
  1. Aflakian S, Zeinali H, Maddah Arefy H, Enteshary Sh, Kaveh, Sh. Study of yield and yield components in 11 ecotype of Thymus daenensis Celak. Iranian J Med Aro Plants. 2012;28:187-197.
  2. Askary M, Behdani M, Parsa S, Mahmoodi S, Jamialahmadi M. Water stress and manure application affect the quantity and quality of essential oil of T. daenensis and T. vulgaris. Industrial Crops Pro. 2018;111:336-344.
  3. Niazian M, Sadat-Noori SA, Abdipour M. Modeling the seed yield of Ajowan (Trachyspermum ammi L.) using artificial neural network and multiple linear regression models. Industrial Crops Pro. 2018;117:224-234.
  4. Zarezadeh, A., S. Mirvakili and M. Arabzadeh. Survey on phenology and acclimatization of medicinal plant species in Yazd province collection. Iranian J Med Aro Plants. 2007;23:204-217.
  5. Jafari AA, Goudarzi A. Genetic variation for yield and its relationships with quality and agronomic traits in 72 ecotypes of alfalfa (Medicago sativa L.). Iranian J Range Forests Plant Breeding and Gen Res. 2007;14:215-229.
  6. Faravani M, Jafari AA, Azizi N, Negari AK. Cluster analysis and heritability of yields and yield components of some Ajwain (Trachyspermum ammi) ecotypes. Zeitschrift Fur Arznei-& Gewurzpflanzen. 2019;24:90-93.
  7. Ibrahim M, Aboud K, Hussein R. Genetic variability and path coefficient analysis in sweet basil for oil yield and its components under organic agriculture conditions. J American Sc. 2011;7:150-157.
  8. Khan F, Ali S, Shakeel A, Saeed A, Abbas G. Genetic variability and genetic advance analysis for some morphological traits in (Brassica napus L.). J Agric Reso. 2006;44:83-88.
  1. Mazid SMS, Mohrir MN, Jadhav RS. Genetic variability, heritability and genetic advance in Okra (Abelmoschus esculentus L. Moench). J of Plant Breeding. 2013;4:1255-1257.
  2. Golestani M, Sahhafi, SR. Evaluation of drought tolerance in Thymus daenensis Subsp. daenensis ecotypes. J Crop Breeding. 2020;12:127-139.
  3. Jackson SAL, Hay RKM. Characteristics of varieties of thyme (Thymus vulgaris L.) for use in the UK: Oil content, composition and related characters. J Hortic Sci. 1994;69:275-281.
Journal of Medicinal plants and By-product
Volume 10, Issue 2
September 2021
Pages 183-192

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