Effect of Trigonella foenum-graecum L. on Immune Function and Liver Activity of Ross 308 Broiler Chickens

Document Type : Research Paper


1 Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran

3 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran

4 Department of Health and Quality Control Faculty of Shahmirzad College of Veterinary Medicine, University of Semnan, Semnan, Iran


In this study, the effect of fenugreek (Trigonella foenum-graecum L.) seed powder and aqueous extract on growth performance, immunity, and liver activity in the ROSS 308 broiler were investigated. A total of 270 day-old broiler chicks were randomly divided into 9 groups (n=30), with three replicate, T1 (control group) fed with a basal diet, T2 fed with basal diet added to Immunofin®, and T3 fed with basal diet added to HEPARENOL® in drinking water based on a producer recommended methods. Other 6 groups, were fed with basal diet added to 3 different level of fenugreek seed powder: T4 (0.1%), T5 (0.2%), T6 (0.3%) and 3 different level of extract in drinking water: T7 (0.1%), T8 (0.2%) and T9 (0.3%) and All groups were used during 42 days of rearing. To evaluate antibody titers against Newcastle disease, Avian Influenza, and Gambro vaccines, Hemagglutination Inhibition and Enzyme-Linked Immunosorbent Assay tests were performed. Administration of fenugreek seed extract and powder according to recommended doses had no significant effect on growth performance; however, it had a significant effect on the antibody levels and the levels of Alanine Aminotransferase and Aspartate Aminotransferase enzymes that highly increase in liver disorder, were significantly decreased by aqueous extract of fenugreek seed in 0.2% and 0.3% (P ≤0.05). Overall, the present study showed that aqueous extract and seed powder of fenugreek could be used as a new approach to increase the level of immunity and to improve liver activity in broilers.


  1. Prajapat UK, Jain D, Kumar S, Sharma N. Proximate composition of some Indian herbs used as feed additive in livestock and poultry. 2018;6:2105-6.
  2. Gaikwad BS, Patil RA, Padghan P V, Shinde SS. Effect of Fenugreek (Trigonella foenum-gracum L.) Seed Powder as Natural Feed Additive on Growth Performance of Broilers. 2019;8:1137-46.
  3. Kalia S, Bharti VK, Gogoi D, Giri A, Kumar B. Studies on the growth performance of different broiler strains at high altitude and evaluation of probiotic effect on their survivability. Sci Rep. 2017;7:1-8.
  4. Zadeh JB, kor NM, Olfati A. The Effects of Different Levels Aqueous Extract of Fenugreek (Trigonella foenum-graecum L.) on Performance and Immune Response of Laying Hens. J Essent Oil-Bearing Plants. 2015;18:1476-81.
  1. Yadav UC, Baquer NZ. Pharmacological effects of Trigonella foenum-graecum L. in health and disease. Pharmaceutical biology. 2014;52:243-54.
  2. Campbell T, Ellis, Christian K. No TitleAvian and Exotic Animal Hematology and Cytology. John Wiley & Sons. 2013.
  3. Beghoul S, Mc A, Benazzouz H, Messai A, Chouah B, Mansour A. Fenugreek ( Trigonella foenum-graecum ): an alternative antistress in broiler chickens in Algeria. 2017;9:64-9.
  4. Duru M, Erdoǧan Z, Duru A, Küçükgül A, Düzgüner V, Kaya DA, et al. Effect of seed powder of an herbal legume fenugreek (Trigonella foenum-graecum L.) on growth performance, body components, digestive parts, and blood parameters of broiler chicks. Pak J Zool. 2013;45:1007-14.
  5. Abed AR, Kadhim FO. Effect of mint and fenugreek and mixture on production and immunity of broilers. J Kerbala Univ. 2014;12:139-47.
  6. Eterradossi N, Saif YM. Infectious Bursal Disease. Dis Poult. 2020:257-83.
  7. Cruz CEB, Freitas ER, Braz N de M, Salles RPR, da Silva ING. Blood parameters and enzymatic and oxidative activity in the liver of chickens fed with calcium anacardate. Rev Cienc Agron. 2018;49:343-52.
  8. Rocha TM, Andrade MA, Gonzales E, Stringhini JH, Santana ES, Gagno Pôrto RN, et al. Liver function and bacteriology of organs in broiler inoculated with nalidixic acid-resistant Salmonella Typhimurium and treated with organic acids. Ital J Anim Sci. 2013;12:353-8.
  9. Zaefarian F, Abdollahi MR, Cowieson A, Ravindran V. Avian liver: The forgotten organ. Animals 2019;9:1-23.
  10. Navarro-Villa A, Mica JH, de los Mozos J, den Hartog LA, García-Ruiz AI. Nutritional Dietary Supplements to Reduce the Incidence of Fatty Liver Syndrome in Laying Hens and the Use of Spectrophotometry to Predict Liver Fat Content. J Appl Poult Res. 2019;28:435-46.
  11. Moradi N, Didarshetaban MB, Reza H, Pour S. Fenugreek (Trigonella foenum-graecum L.) As a Valuable Medicinal Plant. Int J Adv Biol Biomed Res. 2013;1:922-31.
  12. Taebipour MJ, Dadras H, Nazifi S, Afsar M, Ansari-Lari M. Evaluation of blood monocyte and lymphocyte population in broiler chicken after vaccination and experimental challenge with Newcastle disease virus. Vet Immunol Immunopathol. 2017;190:31-8.
  13. Krams I, Vrublevska J, Cirule D, Kivleniece I, Krama T, Rantala MJ, et al. Heterophil/lymphocyte ratios predict the magnitude of humoral immune response to a novel antigen in great tits (Pirus major). Comp Biochem Physiol - A Mol Integr Physiol. 2012;161:422-8.