The Effect of Micronutrients supplementation (Fe, Zn, B, and Mn) on Antioxidant Activity of Milk Thistle (Silybum marianum L.) under Rainfed Condition

Document Type : Research Paper

Authors

1 Department of Agronomy, Faculty of Agriculture, Urmia University, West Azerbaijan, Iran

2 Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, Urmia University, Urmia,, Iran

Abstract

Milk thistle extract is used to treat liver diseases. The aim of this experiment was to study the effects of micronutrients (Fe, Zn, B, and Mn) on antioxidant activity of milk thistle seed. The study was conducted at the Experimental Fields of Agronomy Department, Faculty of Agriculture and the lab of Biology Department, Urmia University, West Azerbaijan, Iran, during 2016-2017, used randomized complete block design in three replications. Control, Fe, Zn, B and Mn were used as fertilizer treatments. According to the results the effect of micronutrients on total phenols and flavonoids content, nitric oxide radical scavenging, super oxide radical scavenging activity, DPPH radical scavenging activity, and Chain-breaking activity was significant (P ≤ 0.01). Total phenolic content under Zn spraying had significant difference than other treatments whereas in terms of flavonoids content, Zn and B spraying was significant. DPPH radical scavenging activity was higher in Mn and Fe using than other treatments as the difference was significant. Application of mentioned micronutrients significantly decreased the percentage of super oxide and nitric oxide radical scavenging activity than control. In general, according to the findings of this study, spraying micronutrients such as Fe, Zn, B and Mn can be useful to increase the antioxidant capacity of milk thistle under dryland conditions.

Keywords

References

1. Schandalik R, Gatti G, Perucca E. Pharmacokinetics of silybin in bile following administration of silipide and silymarin in cholecystectomy patients. Arzneimittelforschung. 1992;42:964-968.
2. Kelly FJ. Dietary antioxidants and environmental stress. Proc Nutr Soc. 2004;63:579-585.
3. Ly TN, Hazama C, Shimoyamada M, Ando H, Kato K, Yamauchi R. Antioxidative compounds from the outer scales of onion. J Agric Food Chem. 2005;52:8183-8189.
4. Salvatore S, Pellegrini N, Brenna OV, del Río D, Frasca G, Brighenti F, Tumino R. Antioxidant characterization of some Sicilian edible wild greens. J Agric Food Chem. 2005;53:9465-9471.
5. Young JA, Evans RA, Hawkes RB. Milk Thistle (Silybum marianum L.) Germination. Weed Sci. 1978;26:395-398.
6. Presser A. Pharmacist's Guide to Medicinal Herbs. Smart Publications, Petaluma, CA. 2000;259-260.
7. Ramasamy K, Agarwal R. Multitargeted therapy of cancer by silymarin. Cancer Lett. 2008;269:352-362.
8. Tsai JH, Liu JY, Wu TT, Ho PC, Huang CY, Shyu JC. Effects of silymarin on the resolution of liver fibrosis induced by carbon tetrachloride in rats. J Viral Hepatitis. 2008;15:508-514.
9. Tuchweber B, Sieck R, Trost W. Prevention by silibinin of phalloidin induced hepatotoxicity. Toxicol Appl Pharmacol. 1979;51:265-275.
1.Magliulo E, Carosi PG, Minoli L, Gorini S. Studies on the regenerative capacity of the liver in rats subjected to partial hepatectomy and treated with silymarin. Arzneimittelforschung. 1973;23:161-167.
2.Mohamed M, El-Fouly Zeinab M, Mobarak and Zeinab A, Salama M. Micronutrients (Fe, Mn, Zn) foliar spray for increasing salinity tolerance in wheat Triticum aestivum L. Afr J of Plant Sci. 2011;5:314-322.
3.Welch RM, Allaway WH, House WA, Kubota J. Geographic distribution of trace element problems. In: Mortvedt JJ, ed. Micronutrients in agriculture, 2nd Ed. Madison, Wisconsin: 1991; SSSA Book Ser. 4. SSSA:31-57.
4.Havlin JL, Beaton JD, Tisdale SL, Nelson WL. Soil Fertility and Fertilizers – An introduction to nutrient management 6th Ed. Prentice Hall, New Jersey. 1999;1034-1056.
5.Widom M, Mihalkovic M. Symmetry-broken crystal structure of elemental boron at low temperature. Phys Rev B. 2008;77:24-34.
6.Farnad N, Heidari R, Aslanipour B. Phenolic composition and comparison of antioxidant activity of alcoholic extracts of Peppermint (Mentha piperita). J. Food Meas Charact. 2014;8:113-121.
7.Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS. Agric J. Food Chem. 1999;57:792-797.
8. Youngjae LRS, Hai F, Jacqueline DH, Nockc BW. Christopher, Postharvest. Biol Technol. 2007;45:349-357.
9. Hatano T, Kagaw H, Yasuhar T, Okuda Chem T. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Pharm Bull. 1988;36:1090-2097.
10. Magliulo E, Carosi PG, Minoli L, Gorini S. Studies on the regenerative capacity of the liver in rats subjected to partial hepatectomy and treated with silymarin. Arzneimittelforschung. 1973;23:161-167.
11. Mohamed M, El-Fouly Zeinab M, Mobarak and Zeinab A, Salama M. Micronutrients (Fe, Mn, Zn) foliar spray for increasing salinity tolerance in wheat Triticum aestivum L. Afr J of Plant Sci. 2011;5:314-322.
12. Welch RM, Allaway WH, House WA, Kubota J. Geographic distribution of trace element problems. In: Mortvedt JJ, ed. Micronutrients in agriculture, 2nd Ed. Madison, Wisconsin: 1991; SSSA Book Ser. 4. SSSA:31-57.
13. Havlin JL, Beaton JD, Tisdale SL, Nelson WL. Soil Fertility and Fertilizers – An introduction to nutrient management 6th Ed. Prentice Hall, New Jersey. 1999;1034-1056.
14. Widom M, Mihalkovic M. Symmetry-broken crystal structure of elemental boron at low temperature. Phys Rev B. 2008;77:24-34.
15. Farnad N, Heidari R, Aslanipour B. Phenolic composition and comparison of antioxidant activity of alcoholic extracts of Peppermint (Mentha piperita). J. Food Meas Charact. 2014;8:113-121.
16. Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS. Agric J. Food Chem. 1999;57:792-797.
17. Youngjae LRS, Hai F, Jacqueline DH, Nockc BW. Christopher, Postharvest. Biol Technol. 2007;45:349-357.
18. Hatano T, Kagaw H, Yasuhar T, Okuda Chem T. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Pharm Bull. 1988;36:1090-2097.
19. Garrat DC. The quantitative analysis of drugs (Chapman and Hall Ltd, Japan,). 1964;211-214.
20. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol. 1995;28:5-30.
21. Jing TY, Zhao XY. The improved pyrogallol method by using terminating agent for superoxide dismutase measurement. Prog Biochem Biophys. 1995;22:84-86.
22. Altaş S, Kızıl G, Kızıl M, Ketani A, Haris PH. Protective effect of Diyarbakır watermelon juice on carbon tetrachloride–induced toxicity in rats. Food Chem Toxicol. 2011;49:2433-2438.
23. Dapkevicius A, Van Beek TA, Lelyveld GP, Van Veldhuizen A, De Groot AE, Linssen JPH, Venskutonis R. Isolation and structure elucidation of radical scavengers from Thymus vulgaris leaves. J Nat Prod. 2002;65:892-896.
24. Shahidi F, Wanasundara PKJPD. Phenolic antioxidants. Crit Rev Food Sci Nut. 1992;32:67-103.
25. Havsteen B. Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharmacol. 1982;32:1141-1148.
26. Wojdyło A, Oszmian´ski J, Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. 2007;105:940-949.
27. Sun J, Li X, Yu X. Antioxidant Activities, Total Flavonoids and Phenolics Content in Different Parts of Silybum Marianum L. Plants. Chem Eng Trans. 2016;55:37-42.
28. Salem N, Dhifi W, Graya A, Mnafeg F, Gharbi S, Khammassi S, Marzouk B, Limam F, Chekir Ben Chaouacha R. Antioxydant activity of Silybum marianum and Ajuga iva natural dyes. Int J Control Autom Syst. 2016;3:6-12.
29. Tupe RS, Kemse NG, Khaire AA. Evaluation of antioxidant potentials and total phenolic contents of selected Indian herbs powder extracts. Int Food Res J. 2013;20:1053-1063.
30. Serçe A,  Çeken Toptancı B, Emen Tanrıkut S, Altaş S, Kızıl G, Kızıl S, M. Assessment of the Antioxidant Activity of Silybum marianum Seed Extract and Its Protective Effect against DNA Oxidation, Protein Damage and Lipid Peroxidation, Food Technol Biotechnol. 2016;54:455-461.
31. Pereira C, Calhelha RC, Barros L, Queiroz MJRP, Ferreira ICFR. Synergism in antioxidant and anti–hepatocellular carcinoma activities of artichoke, milk thistle and borututu syrups. Ind Crops Prod. 2014;52:709-13.
32. Yadegari M. Foliar application of micronutrients on essential oils of borago, thyme and marigold. J Soil Sci Plant Nutr. 2015;15:949-964.
33. Vaya J, Belinky PA, Aviram M. Antioxidant constituents from licorice roots: Isolation, structure elucidation and antioxidative capacity toward LDL oxidation. Free Radic Biol Med. 1997;23:302-313.
34. Sun J, Li X, Yu X. Polysaccharides, total flavonoids content and antioxidant activities in different parts of Silybum marianum L. plants, International Conference on Materials Science, Resource and Environmental Engineering, AIP Conf Proc. 2016;1794:050004-1-050004-4.
35. Jamshidi M, Ahmadi HR, Rezazadeh S, Fathi F, Mazanderani M. Study on phenolic and antioxidant activity of some selected plant of Mazandaran province. Medic Plan. 2010;9:177-183.
36. Beligni MV, Fath A, Bethke PC, Lamattina L, Jones RL. Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone layers. Plant Physiol. 2002;129:1642-1650.
37. Wink DA, Miranda KM, Espey MG, Pluta RM, Hewett SJ, Colton C, Vitek M, Feelisch M,Grisham MB. Mechanisms of the antioxidant effects of nitric oxide. Antioxid. Redox Signal.2001;3:203-213.
38. Moncada S, Palmer RMJ, Higgs EA. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev. 1991; 43:109-142.
39. Ebrahimzadeh MA, Pourmmorad F, Hafezi S, 2008. Antioxidant activities of Iranian corn silk. Turk J Biol. 2008;32:43-49.
40. Saharan BS, Nehra V. Plant growth promoting rhizobacteria: a critical review. Life. Sci. Med. Res. 2011;21:30.‏
41. Saikia LR, Upadhyaya S. Antioxidant activity, phenol and flavonoid content of A. racemosus Willd. a medicinal plant grown using different organic manures. Res. J. Pharmaceutical. Biolog. Chem. Sci. 2011;2:457-463.‏
42. Ibrahim MH, Jaafar HZ. Photosynthetic capacity, photochemical efficiency and chlorophyll content of three varieties of Labisia pumila Benth. exposed to open field and greenhouse growing conditions. Acta. Physio. Plantarum. 2011;33:2179-2185.‏
43. Nejatzadeh-Barandozi F, Gholami-Borujeni F. Application of different fertilizers on morphological traits of dill (Anethum graveolens L.). Org. Medic. Chem. Letters. 2014;4:4.
44. Javanmardi J, Khalighi A, Kashi A, Bais HP, Vivanco JM. Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran. J. Agric. Food. Chem. 2002;50:5878-5883.
Journal of Medicinal plants and By-product
Volume 10, Special
April 2021
Pages 43-50

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